关键词: 家蝇, 抗菌物质, 诱导, 针刺

Abstract: Objective To investigate the kinetics of the inducible antibacterial activity of the hemolymph in larvae of the housefly.Methods The third instar larvae were induced by pricking,living rates of the pricked larvae were recorded at different times after treatment,and hemolymphs collected at 0,24,48,72 hours were tested against Staphylococcus aureus and Micrococcus lysodeikticus respectively.The diameters of clear inhibitory ring were observed and recorded to express antibacterial activity.Results The living rates of the larvae declined gradually.The antibacterial activity peak appeared across 48 hours after treatment.Conclusion It implies that the antibacterial substance was induced in the housefly after being pricked.The immunized hemolymph should be collected at 48 hour after treatment.