家蝇成虫抗菌肽对4种肿瘤细胞的作用观察

中国媒介生物学及控制杂志

家蝇成虫抗菌肽对4种肿瘤细胞的作用观察

赵瑞君1;张庆华1;李飞栋2

1西医科大学寄生虫教研室太原030001;2山西省肿瘤医院

出版日期:2007-02-20 发布日期:2007-02-20

The effection of Antimicrobial peptides extracted from adult housefly on tumour cell

ZHAO Rui-jun; ZHANG Qing-hua; LI Fei-dong

Department of Parasitology, Shanxi Medical University, Taiyuan 030001, China

Online:2007-02-20 Published:2007-02-20

摘要/Abstract

摘要： 目的观察家蝇成虫抗菌肽对肿瘤细胞的作用效果。方法针刺感染大肠埃希菌诱导家蝇成虫产生抗菌肽,经研磨、离心、层析等步骤提取其抗菌肽,将其作用于肿瘤细胞109、K562、Daudi及T24,以流式细胞仪检测其作用效果。结果该抗菌肽对4种肿瘤细胞的有效杀伤力M1均在85%以上。结论家蝇成虫抗菌肽可作为潜在的抗肿瘤药物开发。

关键词: 家蝇成虫, 抗菌肽, 抗肿瘤

Abstract: Objective In this paper,the action of the antimicrobial peptide of adult housefly (Musca domestica) against some tumor cell was investigated by Flow Cytometer(FCM). Methods The antimicrobial peptides from adult housefly was induced by Escherichia coli with a needle and was isolated and purified by grinding,centrifugalizing and chromatographing,the action of the peptide against tumor cells 109,K562,Daudi and T24 was observed by FCM. Results The present study demonstrated that the peptides could kill above four kinds of tumor cells to some extent,and M1 were all above 85%. Conclusion The antitumor action of antimicrobial peptide of adult housefly could make it possible to develop a new kinds of anti-tumor drug.

赵瑞君1;张庆华1;李飞栋2. 家蝇成虫抗菌肽对4种肿瘤细胞的作用观察[J]. 中国媒介生物学及控制杂志.

ZHAO Rui-jun; ZHANG Qing-hua; LI Fei-dong. The effection of Antimicrobial peptides extracted from adult housefly on tumour cell[J]. Chines Journal of Vector Biology and Control.

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