关键词: 凝血反应, 黄胸鼠, 抗药性, 溴敌隆, 区分剂量

Abstract: Objective To develop techniques to monitor the resistance of commensal rodents with blood clotting response(BCR) test. Methods Buff-breasted rats Rattus flavipectus were sampled from suburbs of Zhanjiang,Guangdong province,where higher resistance to bromadiolone had been documented previously. Results With non-alternative feeding tests,the upper limit of 95% effective dose of the wild population was proposed as discrimination dose(4.0 mg/kg) to discriminate the resistant buff-breasted rat form susceptible ones according to the method appraised by European and Mediterranean Plant Protection Organization(OEPP/EPPO,1995). And then,effects of bromadiolone at 4.0 mg/kg body weight on the blood clotting response of buff-breasted rat were studied. Results showed that the discrimination dose droved the percentage clotting activity(PCA) distribution pattern changed form one peak type of pre-treatment whole population into double peaks type,which one peak stand for resistant individuals which would survive within 20 d for their PCA range from 10-100,and the other peak should be susceptible individuals which would die in the following days for their PCA less than 10,which indicated the consistence of BCR method and non-alternative feeding test in monitoring the resistance of buff-breasted rat. Furthermore,both resistant and susceptible individuals decreased their PCA in the following days,while PCA of resistant individuals only decreased to 17% of normal level and soon increased to normal level within 2-3 d,compared to that of susceptible individuals whose PCA decrease dramatically and no increase observed. Therefore,PCA=10(INR=5.0) might be a threshold in determining "responders" to discriminate the resistant and susceptible rodent administrated by 4.0 mg/kg bromadiolone. Conclusion BCR might be applicable method to monitor the resistance against anticoagulant rodenticides of commensal rodents.