关键词: 褐家鼠, 汉坦病毒, 基因亚型, 地理分布

Abstract: Objective To investigate the subtypes and distribution of Seoul virus in Jilin province. Methods Rats were collected from the main endemic areas in Jilin and Hantavirus in the lung were identified by indirect immunofluorescence assay. M and S segments of positive samples were partially amplified with nested RT-PCR and sequenced. Phylogenetic analysis was used for genotyping. Results All hantaviruses from Rattus norvegicus were Seoul virus in the main endemic areas of Jilin. Phylogenetic analysis of partial M and S segments showed that Seoul hantaviruses carried by R.norvegicus in Jilin were the S3 sub-genotype,and were closely related with the strains from Liaoning and Heilongjiang provinces. Conclusion The subtype of Seoul hantaviruses in Jilin province was S3.