关键词: 汉坦病毒, 啮齿动物, 流行病学

Abstract: Objective In order to make tracks for infectious factors of hemorrhagic fever with renal syndrome(HFRS),and to investigate hantavirus(HV) infection of rodents from urban district of Beijing. Methods We trapped the rodents in different niches in urban district monthly during 2003-2004,detected the IgG reacting sera to HV antigen using ELISA,and amplified the partial M segment of HV from captured rodent lung samples with RT-PCR and then sequenced it directly. The alignment of the nucleotide and the deduced amino acid sequences,together with phylogenetic analysis were made with DNASTAR software. Results 2 species (Rattus norvegicus and Mus musculus) of 229 rodents were captured. The overall seroprevalence of HV infection was 4.37% (10/229) with R.norvegicus 3.51%( 6/171) and M.musculus 6.90%( 4/58) respectively. RT-PCR positive rates were 0.87%( 2/229) with R.norvegicus 0.58%( 1/171) and M.musculus 1.72%( 1/58) respectively. The nucleotide sequences of 356 bp region (1958-2313) of M segment obtained from these 2 samples were all identified to SEOV,with 5.40% divergence. Phylogenetic analysis revealed that they all belonged to Z37 subgenotypes from Zhejiang province,and could be branched into 2 different subclades. Conclusion It showed SEOV were the dominant genotypes in Murinae rodents from urban district of Beijing and had the variational nature among different hosts. The HV prevalence of hosts in urban district was not high. Besides R.norvegicus,the increasing prevalence effect of M.musculus should be noticed.