关键词: 家蝇幼虫, 抗菌蛋白, 黑色素瘤细胞, 细胞凋亡

Abstract: Objective To investigate the inducement of the antibactarial materal from the Musca domestica larvae to the A₃₇₅ apoptosis to explore it is antitumor mechanism.Methods The effects of the antibactarial materal from the(M.domestica) larvae on the cell cycle and apoptosis of A₃₇₅ were measured by flow cytometry.The morphology of apoptosis cells was observed with the hematoxylin-eosin stainings,and the apoptosis index(AI) was measured.Results Of the A₃₇₅ cell cycle,percentage of G₂/M was increased,and the percentage of S was decreased in the group treated by 0.10% the antibactarial material.The percentage of G₀/G₁,G₂/M and AI/PI were much increased,both the percentage of S and PI were much decreased in the(0.50%) group.In the 2.50% groups,the percentage of S,PI,AI/PI and apoptosis were significantly increased,the percentage of G₀/G₁ was much decreased.At the 12.50% group,the percentage of G₂/M,PI,AI/PI and apoptosis were significantly increased,the percentage of G₀/G₁ and S were much decreased.Conclusion The antibactarial materal of the M.domestica larvae could inhibited and killed the A₃₇₅ cell.The growth of A₃₇₅ may be inhibited by the antibactarial materal blocking cell cycle and inducing the apoptosis of cell.