关键词: 鼠疫菌, 生化特性, 河北

Abstract: Objective To research the biochemical characters of 27 Yersinia pestis,so as to get their basic data of biochemical information. Methods To determine the capability 27 plague strains to ferment carbohydratealcohols and biochemical reaction of with testtube method of liquid nutrient medium and solid culture medium (slant or highlayer). Results (1)The tested Y.pestis fermented Glycerol,Larabinose,etc. which was acidgenic and aerogenic and didn't ferment Maltose,Rhamnose. Fermenting Melibiose was negative in 72 h and part fermentstube of 8 strains appeared positive after 5 d. (2)Biochemical reaction:The results of 27 strains showed negative reaction in the reducing KNO₃,nitrate reaction,V-P experiment,urea decomposition,indole formation,liquefies gelatin,utilizes citrate and growth test on nonpeptone acid agar medium. Methyl red experiment was positive and they were able to slightly produce H₂S. Conclusion According to Chinese Y.pestis ecotype typing standard,the strains belong to B group,Erduosi Plateau,and their principal biochemical characters correspond with Y.pestis isolated from the focus in the past.