关键词: 鼠疫耶尔森菌, 土壤, 动物脏器, 荧光定量PCR

Abstract: Objective To establish and assess different methods for detection of Yersinia pestis from artificially contaminated soil and infected mice tissues by realtime fluorescence polymerase chain reaction. Methods Different methods for isolation and purification of nucleic acids from soil and mice tissues contaminated by Y.pestis vaccine strain were established. Three combinations of primers and probes designed according to caf1 and pla and hms genes of Y.pestis were used to assessed these methods. The optimal systems were confirmed by comparing the amplification results. Results The method of NaIGlassmilk is the most effective,and ten thousand of bacteria in one gram soil could be directly detected by this system. Conclusion The NaIGlassmilk method and the Realtime fluorescence polymerase chain reaction could directly and rapidly detect Y.pestis in contaminated issues or soil.