关键词: 青海血蜱, 日本血蜱, 随机扩增多态DNA

Abstract: Objective To differentiate H.qinghaiensis and H.japonica by random amplified polymorphic DNA. Methods Genome DNA was extracted from the two species of ticks,the total volume of reaction and conditions of reaction were defined. Eight primers with different polynucleotide sequences were selected,the genome DNA of the two species of ticks was amplified DNA fingerprint maps were made with the amplified products. The polymorphism of DNA of the two species of ticks was compared,the two species of ticks were identified on DNA level. Results Only minor DNA fragments of the two species of ticks are the same,the major DNA fragments of them are different. Conclusion RAPD can be used to identify H.qinghaiensis and H.japonica.