关键词: 鼠形动物, SARS冠状病毒, 特异性抗体

Abstract: Objective To grasp the level of SARS coronavirus specific IgG in the sera of rat-shaped animals in Guangdong province and provide reference for exploring the relativity between rat-shaped animals and SARS prevalence.Methods Rat-shaped animals were captured in the places related or probably related to SARS prevalence in Guangdong.The sera were taken and the level of SARS specific IgG were detected by SPA-ELISA and conventional ELISA.Results Totally 193 cases of sera were acquired from the captured rat-shaped animals including Rattus flavipectus,R.norvegicus,Mus musculus and Suncus murinus.The detection results by SPA-ELISA showed that 7 cases were SARS specific IgG positive with a detection rate of 3.6% and all were Rattus flavipectus or R.norvegicus.139 cases of Rattus flavipectus.and R.norvegicus sera were retested by conventional ELISA,showing 18 positive with a detection rate 12.9%.Of the positive cases by SPA-ELISA,5 were retested by conventional ELISA and 4 still showed positive.All positive cases were from the three cities,Guangzhou,Foshan and Shenzhen.Conclusion Of the captured rat-shaped animals in Guangdong province,the SARS coronavirus specific IgG posive cases were all Rattus flavipectus or R.norvegicus,and it seemed that the area distribution of the positive cases had certain relativity with some of SARS endemic features.The sensitivity of SPA-ELISA for SARS coronavirus specific IgG detection in rats is probably lower than conventional ELISA.However,the relative intensity of the IgG positive cases with SARS coronavirus infection and bearing still needs more research to confirm.