关键词: 微波免疫荧光方法, 肾综合征出血热抗体, 狂犬抗体

Abstract: Objective Developing microwave immunofluorescence method for quick detection of hemorrhagic fever with renal syndrome(HFRS) and rabies virus antibody.Methods Screening out the best reaction conditions of the microwave immunofluorescence method by direct immunofluorescence technology;Examining HFRS antibody in 50 serum samples from suspicious HFRS patients and rabies antibody in 50 serum samples from human 10 days after 5 injections of rabies vaccine with the microwave immunofluorescence method and comparing the results with those of the routine indirect immunofluorescence test.Results The best reaction condition was treatment with 40% microwave intensity in 1 min and the secondary was treatment with 20% microwave intensity in 2 min.The others were imprecticable.16 samples were judged positive of HFRS antibody(GMT=160.0) by the microwave immunofluorescence method and the positive rate was 32%.The result was just the same as that of the routine method(GMT=167.1) and they had no considerable difference statistically.43 samples were judged positive of rabies antibody(GMT= 83.93) by microwave immunofluorescence method and the positive rate was 86%.Comparing with the positive number of 45(GMT=87.70)and the positive rate of 90% resulted by the routine method,the result showed no considerable statistic difference.Conclusion The microwave immunofluorescence method is quick and easy with high sensitivity and specificity.It can be used for quick diagnosis and is applicable in base units.