关键词: 甲氧杀虫酯, 药效, 降解, 对酯酶的影响

Abstract: Methoxy phenyl Acetate which was screened from study on the relationship between the chemical structure of 1-(3, 4-dichlorophenyl)-2, 2, 2-triehloroethyl acetate analogues and insecticidal activities is a new type of insecticide to mosquito and housefly. The LD₅₀ to houseflies (Musea domestica vicina L,) is 0.3 lug/hody. The KT₅₀ to mosquitoes (Aedes aegypti L.) is 1.77 min. and 3.22 min. (Culex pipiens var. pallens) respectively. No effect of this compound on the activities of acetylcholinesterase and carboxylesterase releasing from serum has been determinted, the serum used come from mice which was treated with methoxyphenyl acetate ench day stccessively for 20 days. The activity of esterase from serum(enzyme source is same as AchE) and liver tissue were analyzed by SDS-gel electrophoresis, the result showed that there is no inhibition of this compound to esterase activity.Rate of Biodegradation of Methoxyphenyl Acetate was carried out in special apparatus, 5ml of Waymouth's medium or the medium containing the desired chemical (1×10^-4 mol/L) was introduced into each incubation flask. The same volume of Waymouth's medium containing hepatocytes was then transferred from the maintance flask to the incubation at 37℃, 0.4ml aliquot were removed at intervals and centrifuged (2000 rpm/min.) for 5 sec. A 0.2ml aliquot of supernatant fluid was carefully removed into a tube containing 0.1ml of TCA(10%) and added 2ml of hexane for extraction. The extract was stored at-20℃ until GC analysis. It has been shown that the rate of biodegradation of Methoxyphenyl Acetate was 72.5% and 93% within 45 min. and 90 min. respectively. It seems to be biogradated with ease.