关键词: 嗜人按蚊, 蚊, 细胞系, 细胞培养

Abstract: A cell line from Anopheles anthrophagus-Ana 104 is established from 1st instar larvae. The medium is TC199, supplemented with 0.4% Lactabumin Hydrolysate (LH), 0.03% glutamine, 10-20% FBS and adequate amount of penicillin and streptomycin as well as non essential amigo acids. The fragment of the larvae remained active for 2.5 months and continuously produced cells. After 7-10 days of subculture, the cells formed a moaolayer which could be maintained for more than 22 days. The cell line is on its 27 passage leveal now. The cell is adapt to the medium L-15, DMEM etc. From Oth passage on some cells were stored in liquid nitrogen and successfully resuscitated. Ana 104 mainly contains epitherial type cells, spindal like cells also exist. The chromosome number is 2n=6, The observation with electron microscope showed no contamination of virus or micoplasma. The dynamic study of amino acids requirement indicated that the cells consumed relatively large amount of Glu. , Tyr. , Orn. , Arg. , Cys. , etc, but increased the concentration of Asp. in the medium. The activity of carboxylesterase and cholinesterase were detected. Esterase Zymograms form polyacrylamide gel electrophoresis revealed 3 bands.The Anopheles anthropophagus is affidicted to human blood, it is important vecor in China. Ana 104 was the 4th cell line established from Anopholine mosquito, The other three was established from An. gambia, An. stephensi, An. sinensis. The cell line Ana 104 would be helpful in the study of virology, parasitology, cell and molecular biology and its management.