关键词: 莱姆病, 全沟硬蜱, 经期传播莱姆病螺旋体

Abstract: Objective:To determine whether I.persulcatus can transmit Lyme spirochetes Borrelia garinii trans-stadially.Methods:Larva of I.persulcatus were allowed to feed on the KM mouse infected with tick-derived Lyme spirochete Borrelia garinii.And the larva replete and resultant nymphs were detected for the spirochetes by cultivation and PCR screening.Results:Almost all the larva of I.persulcatus replete from KM mice can acquire the spirochetes.During the blood ingestion period followed,the larva can maintain the infection,though its positive infection ratio fluctuated.After molted,about 45.0% of quest nymph I.persulcatus detected was infected with the spirochetes,which were infectious to the sensitive KM mouse.When fed by the nymph,the sensitive KM mouse should be infected by the spirochetes.The ear lope kidney spleen and bladder of the KM mouse were also infected.Conclusion: I.persulcatus is able to acquire and trans-phase transmit the spirochete B.garinii from larva to nymph stage.