关键词: 嗜人按蚊, 基因组, RAPD分析

Abstract: Objective:In order to provide the scientific basis for determinating furtherly whether there are different sub-species in Anopheles anthropophagus in China.Method:DNA fingerprint maps of Anopheles anthropophagus from Sichuan,Yunnan and Jiangsu provinces were drawn by using random amplified polymorphic DNA(RAPD) technique.Diversities among the different strains of Anopheles anthropophagus were analyzed at genomic DNA level by identifying their DNA polymorphism.Results:In spite of the overwhelming likeness among DNA fragments of the three strains,there still existed some discrepancies.The common fragments indicated some degree homogeneity of the three strains'genomes,their distinctive fragments and intensity suggested the diversity among the strains.Conclusion:At DNA level,although they were mostly alike,diversities among the different strains of Anopheles anthropophagus still existed,which can provide the scientific basis of molecule classification for identification of Anopheles anthropophagus geographical sub-species that can not be discriminated by morphology.