关键词: 昆虫, 饲养

Abstract: Objective:In order to study experimental bacteria carriage in insects,we developed an experimental breeding technique for an insect in a single tube.Methods:Bacterial suspension were compounded by mixture of broth、Tween-80 and food oil,dripping on the external of flies and cockroaches,then,moving each of them separately into a glass tube with a cap having 3 or 5 holes.Results:The mixture of bacterial suspension was a good adhesive agent for insects.The flies recovery rate was 74.0%,and the cockroaches recovery rate 58.0%(P=0.1395);the flies survival rate was 43.24%,and cockroaches the survival rate were 48.28%(P=0.7841) in 14th day.Conclusions:This technique can drip fixed quantity of the bacteria onto insects,having no cross infection happened among insects during experimental period.It is a simple method for breeding insects,and is fit for the research of vector transmission diseases by insects.