关键词: 滤纸法, 淡色库蚊, 非特异性酯酶, 乙酰胆碱酯酶

Abstract: Objective:To establish the filter paper test for the determination on non-specific esterase and acetylchotin esterase as a resistant determination method.Method:Filter paper test.Result:Filter paper test was used in this study to test the non-specific esterase(NSE)and acetylchotin(AchE)activities of susceptible and resistant strain of Cx.pipiens pallens,so as to determine the resistant level of individual mosquito according to colour changes.The results indicated that susceptible mosquitoes showed no or slight colour reaction while resistant mosquitoes revealed deep colour,and the NSE and AchE activity was inhabited in susceptible individuals by insecticides,while it cannot be inhabited in resistant individuals.It was demonstrated that there existed the difference in the AchE activity between susceptible and resistant mosquitoes.Conclusion:This method can rapidly determine the NSE and AchE activity in mosquitoes,and easy to perform,so it can be used for qualitatively determining the resistant level of mosquitoes and be convenient to be popularized in the field.