中国媒介生物学及控制杂志

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微小按蚊rDNA内转录间隔2区序列差异

王学忠1;HaroldTownson2;王丕玉1;李菊升1   

  1. 1云南省疟疾防治研究所 思茅665000;2英国利物浦大学热带病学院
  • 出版日期:2001-02-20 发布日期:2001-02-20

Sequence Differences of Second Internal Transcribed Spacer of Ribosomal DNAin Anopheles minimus

WANG Xue-zhong*; Harold Townson; WANG Pei-yu; et al   

  1. Yunnan Institute of Malaria Control,Simao 665000
  • Online:2001-02-20 Published:2001-02-20

摘要: 目的:比较不同地株微小按蚊核糖体DNA(rDNA)内转录间隔2区(ITS2)序列差异。方法:通过对PCR扩增rDNAITS2片段测序,比较其不同地株差异。结果:对6株微小按蚊测序比较,存在有微小按蚊A和C型。结论:rDNAITS2序列差异可用于建立A、C型的分子鉴别方法。

关键词: 微小按蚊, DNA, 核糖体, 第2内转录间隔区

Abstract: Objective:To compare the sequence difference of the ribosomal DNA second internal transcribed spacer(ITS2) in the different strains of Anopheles minimusin Yunnan.Methods:The PCR amplified rDNA ITS2 sequences,to compare the variation of An.minimus.Results:The different strains of 6 populations were sequenced,which could be separate into Anopheles minimusA and C.Conclusion:The sequencedifference of the rDNA ITS2 would be very useful for the developing a molecular approach to distinguish An.minimusA and C.