中国媒介生物学及控制杂志

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革螨、恙螨体内肾综合征出血热病毒结构蛋白及基因检测

张云1; 朱进1; 吴光华1; 章莉莉2; 孙志坚2; 张家驹3; 周燕萍3   

  1. 1南京军区军事医学研究所210002; 2南京医科大学传染病学教研室; 3陕西省卫生防疫站
  • 出版日期:1999-08-20 发布日期:1999-08-20

Detection of Hemorrhagic Fever with Renal Syndrome Virus Structu ral Protein and Gene in Gamasid mite and Chigger Mite

Zhang Yun; Zhu Jin; Wu Guanghua; et al   

  1. Institute of Military Medicine, Nanjing Command,Nanjing 210002
  • Online:1999-08-20 Published:1999-08-20

摘要: 目的:对革螨、恙螨体内HFRS病毒结构蛋白及基因进行检测。方法:采用免疫组化法和套式PCR检测。结果:革螨、恙螨体内均可检测到HFRSV结构蛋白MP、NP片段,而恙螨体内HFRSV随滴度的增高其检出强度亦增加。结论:用分子生物学方法进一步证明革螨、恙螨体内存在HFRSV。

关键词: 肾综合征出血热病毒, 结构蛋白, 革螨, 恙螨

Abstract: In order to verify the existence of hemorrhagic fever with renal syndrome virus (HFRSV) in gamasid mite and chigger mite,structu ral protein (MP and NP) of HFRSV were detected by immunological histochemistry a nd HFRSV-RNA were amplified by RT-PCR. The results show that MP and NP of HFRSV were existed in larva and adult of gamasid mite and chigger mite, and positive p ercentage of detection were higher as the titre of HFRSV in gamasid mite and chi gger mite were bigger. It demonstrated the exitence of HFRSV in gamasid mite and chigger mite by molecular biology furtherly.