关键词: 微板法, 蚊虫, 乙酰胆碱酯酶

Abstract: Microplate test was employed for the detection of acetylcholinesterase(AchE) level in susceptible mosquitoes and resistant strains of Culex pipiens pallens.Among 4 species of susceptible mosquitoes,Anopheles sinensis produced the highest level of AchE. The AchE level varied in different resistant strains of Cx. pipiens pallens, with anti propoxur strain the highest level,foollwed by anti-cypermethrin strain and with anti-DDVP strain the lowest. The AchE activity increased with the development of mosquito. Using propoxur(at 0.01mmol/L) as the inhibitor , the inhibition rate of AchE was highest in susceptible strain,followed by anti-cypermethrin strain ,anti-DDVP strain and anti-propoxur strain ,in descending order.The sensitivity of AchE was higher in susceptible strain than in resistant strains.