关键词: 肾综合征出血热病毒（HFRSV）, 反转录聚合酶链反应, 点印迹杂交

Abstract: Four oligonucleotides flanking the M segment were chosen as primers.RNA,extracted by singlestep method of acid guanidinium thiocyanate phenolchloroform.A reverse transcriptionpolymerase chain reaction (RTPCR) and Nested RTPCR were adopted for detection of HFRSV in chigger mite.The PCR products was then detected by agarose gel electrophoresis and dotblot hybridization.The results showed that 50、10 mites from Ag positive mice,50 free mites were positive by RTPCR,5 mites from Ag positive mice,50、10 mites from Ag negtive mice,10,5 free mites were positive by Nested RTPCR while the normal mouse lung showed negtive result.The results by dotblot hybridization showed positive results with all above.It was suggested that the detection method of Nested RTPCR be simple,sensitive,specific and give further evidence for chigger mite as a vector of HFRSV.