中国媒介生物学及控制杂志

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用鸡胚卵黄囊分离斑点热群立克次体的研究

毕德增; 张健之; 范明远   

  1. 中国预防医学科学院流行病学微生物学研究所
  • 出版日期:1997-02-20 发布日期:1997-02-20

Studies on Isolation SFG Rickettsiae Using Embryonated Hen's Eggs.

Bi De-zeng; Zhang Jian-zhi; Fan Ming-yuan   

  1. Department of Rickettsiology,Institute of Microbiology and Epidemiology,Chinese Academy of preventive Medicine Beijing,102206
  • Online:1997-02-20 Published:1997-02-20

摘要: 本文在用传统的实验动物感染法分离立克次体同时,并尝试用鸡胚卵黄囊培养法直接分离立克次体。结果:用前者未分离出立克次体,而用后者分别从来自北京昌平县的中华革蜱和来自黑龙江省虎林县的嗜群血蜱中各分离到1株立克次体。经鉴定,这两株立克次体属于斑点热群立克次体。结论:鸡胚卵黄囊培养法可以直接从现场节肢动物标本中分离病原体。

关键词: 立体次体, 分离 立克次体, 分离

Abstract: Embryonated hen's eggs was tried to use to isolate sPOtted fever group (SFG) rickettsiaedirectly from the field samples while experimental animal was using.Two strains of SFG rickettsiae wereisolated directly from the field samples by using embryonated hen's eggs, no rickettsia was isolated by using experimental animal.The result suggested that embryonated hen's eggs couldbe used to isolate SFGrickettsiae directly from the field samples.