Abstract:

　   【Abstract】　Objective　To study the gene of 21 Qinghai-Tibetau plateau strains of Yersinia pestis isolated from the different reservoirs in Qinghai, Tibet and Gansu, and to know the relationship of these strains from the different reservoir. Methods　Random amplified polymorphism DNA （RAPD） was used in this study. Results　The electro-phoretic band of amplified products from 16007, 28001 and 01080 Y.pestis strains were similar, but there were difference between three strains and other sample strains. Conclusion　21 strains isolated from the different reservoir were homologous in genetics.

Key words: Random amplified polymorphism DNA, Reservoir, Qinghai-Tibetau plateau strain of Yersinia pestis

摘要：

　　 【摘要】　目的　对青海、西藏和甘肃省（自治区）境内不同宿主体内分离的21株青藏高原型鼠疫耶尔森菌（鼠疫菌）的基因进行分析，旨在比较不同宿主鼠疫菌之间的关系。方法　采用随机扩增多态性DNA（RAPD）技术。结果　扩增产物在凝胶电泳上显示的条带，其中16007、28001、01080号菌株相同，且与其余18株菌株存在差别。结论　该实验中不同宿主体内检出的21株青藏高原型鼠疫菌在遗传学上属于同源。

关键词: 随机扩增多态性DNA, 宿主, 青藏高原型鼠疫菌 ,