A review of detection method of West Nile virus

Abstract

摘要：

　　【摘要】　西尼罗病毒（WNV）是一种虫媒病毒，蚊虫尤其是库蚊是其主要传播媒介，它可以导致人和动物严重的神经系统症状。WNV分布广泛，已在非洲、欧洲、澳洲、亚洲、中东、北美等地区引起流行，给发病国家造成了重大危害，引起世界各国研究者的广泛关注。阻断WNV的传入，为防止该病毒在我国的暴发流行，已成为我国卫生工作者的当务之急。加强病毒的监测，熟悉病毒的实验室诊断方法并将其应用在监测工作中就显得尤为重要。现就WNV感染的病原学检测方法加以综述以供参考。

关键词: 西尼罗病毒, 病原学, 诊断

CLC Number:

R373.3

CHEN Xiao, XIE Peng. A review of detection method of West Nile virus[J]. Chines Journal of Vector Biology and Control, 2009, 20(3): 265-267.

陈晓，谢鹏. 西尼罗病毒感染的病原学检测方法[J]. 中国媒介生物学及控制杂志, 2009, 20(3): 265-267.

References

［1］ Lanciotti RS， Ebel GD， Deubel V， et al. Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States， Europe， and the Middle East ［J］. Virology， 2002，298（1）：96-105.
［2］魏荣，王志亮，李其平. 在中国可以传播西尼罗河病毒的蚊虫种类［J］. 中国媒介生物学及控制杂志， 2004，15（1）：65-66.
［3］ Huang C， Slater B， Rudd R， et al. First isolation of West Nile virus from a patient with encephalitis in the United States［J］. Emerg Infect Dis， 2002，8（12）：1367-1371.
［4］ Lvov DK， Butenko AM， Gromashevsky VL， et al. Isolation of two strains of West Nile virus during an outbreak in southern Russia， 1999 ［J］. Emerg Infect Dis， 2000，6（4）：373-376.
［5］ Turell MJ， Morrill JC， Rossi CA， et al. Isolation of West Nile and sindbis viruses from mosquitoes collected in the Nile Valley of Egypt during an outbreak of Rift Valley fever ［J］. J Med Entomol， 2002，39（1）：248-250.
［6］任军. 西尼罗病毒研究进展［J］. 生命科学， 2005，17（5）：445-448.
［7］ Kauffman EB， Jones SA， Dupuis AP 2nd， et al. Virus detection protocols for west nile virus in vertebrate and mosquito specimens ［J］. J Clin Microbiol， 2003，41（8）：3661-3667.
［8］ Pisarev VB，   Butenko AM， Petrov VA，   et al.   Morphological   and immunohistochemical changes in tissues of the lung， myocardium and kidney in experimental West Nile fever［J］. Vopr Virusol， 2005，50 （2）：37-38.
［9］ Gibbs SE， Ellis AE， Mead DG， et al.   West Nile virus detection in the organs of naturally infected blue jays （Cyanocitta cristata）［J］. J Wildl Dis， 2005，41（2）：354-362.
［10］ Sampson BA， Ambrosi C， Charlot A， et al. The pathology of human West Nile virus infection ［J］. Hum Pathol， 2000，31（5）：527-531.
［11］佟颖，曾晓芃，刘婷，等. 两种快速检测西尼罗病毒方法的研究［J］. 中国媒介生物学及控制杂志， 2006，17（4）：307-310.
［12］ Stone WB， Therrien JE， Benson R， et al. Assays to detect West Nile virus in dead birds ［J］. Emerg Infect Dis， 2005，11（9）：1770-1773.
［13］ Burkhalter KL， Lindsay R， Anderson R， et al.   Evaluation   of commercial assays for detecting West Nile virus antigen［J］. J Am Mosq Control Assoc， 2006，22（1）：64-69.
［14］ Lanciotti RS， Kerst AJ， Nasci RS， et al. Rapid detection of West Nile virus from human clinical specimens， field?collected mosquitoes， and avian samples by a TaqMan reverse transcriptase-PCR assay［J］. J Clin Microbiol， 2000，38（11）：4066-4071.
［15］ Johnson DJ， Ostlund EN， Pedersen DD， et al. Detection of North American West Nile virus in animal tissue by a reverse transcription nested polymerase chain reaction assay［J］. Emerg Infect Dis， 2001， 7（4）：739-741.
［16］张久松，张泮河，左曙青，等. 西尼罗病毒RT-PCR检测方法的建立及其初步应用［J］. 中国人兽共患病杂志， 2004，20（9）：737-740.
［17］邓永强，姜涛，范宝昌，等. 西尼罗病毒的RT-PCR检测与鉴定［J］. 微生物学免疫学进展， 2004，32（4）：31-35.
［18］姜焱，张常印，陈国强. 西尼罗病毒RT-PCR检测方法的建立［J］. 中国动物检疫， 2005，22（5）：25-27.
［19］ Wong ML， Medrano JF. Real-time PCR for mRNA quantitation ［J］. Biotechniques， 2005，39（1）：75-85.
［20］郑夔，周惠琼，柯昌文. TaqMan MGB Real-time RT-PCR检测西尼罗病毒方法的建立［J］. 中国病原生物学杂志， 2008，3（3）：170-172.
［21］徐昌平，卢亦愚，严菊英，等. TaqMan荧光定量RT-PCR快速检测西尼罗病毒的研究［J］. 中国媒介生物学及控制杂志， 2008，19（2）：135-137.
［22］秦成峰，秦鄂德. 多重PCR及其在病毒性疾病诊断中的应用［J］. 生命的化学， 2005，25（5）：402-404.
［23］姜涛，邓永强，于曼，等. 3种重要脑炎病毒的多重RT?PCR检测方法的建立［J］. 军事医学科学院院刊， 2007，31（2）：218-220.
［24］ D’Souza DH， Jaykus LA. Nucleic acid sequence based amplification for the rapid and sensitive detection of Salmonella enterica from foods ［J］. J Appl Microbiol， 2003，95（6）：1343-1350.
［25］ Deiman B， van Aarle P， Sillekens P. Characteristics and applications of nucleic acid sequence-based amplification（NASBA）［J］. Mol Biotechnol， 2002，20（2）：163-179.
［26］ Lanciotti RS， Kerst AJ. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses ［J］. J Clin Microbiol， 2001，39（12）：4506-4513.

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