Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides

doi:10.11853/j.issn.1003.8280.2023.06.002

Abstract

Abstract: Objective To establish the sensitive baseline of houseflies to six commonly used insecticides, so as to provide a basis for the monitoring and control of the insecticide resistance of houseflies.Methods The topical application method was used to test the sensitivity of laboratory susceptible housefly strains to six insecticides (deltamethrin, beta-cypermethrin, permethrin, dichlorvos, propoxur, and dinotefuran) of four categories. Data from previous literature were collected for comparison. The statistical significance of sensitivity differences was determined according to whether the 95% confidence interval of the median lethal dose (LD₅₀) overlapped. Based on the above steps, the baseline sensitivity of houseflies to the six insecticides was established.Results The baseline sensitivity of the six test insecticides against houseflies were as follows: deltamethrin (LD₅₀=0.002 236 μg/♀, y=13.76+5.19x), beta-cypermethrin (LD₅₀=0.003 692 μg/♀, y=9.34+3.84x), permethrin (LD₅₀=0.007 152 μg/♀, y=10.03+4.67x), dichlorvos (LD₅₀=0.074 20 μg/♀, y=5.02+4.44x), propoxur (LD₅₀=0.415 9 μg/♀, y=0.93+2.43x), and dinotefuran (LD₅₀=0.103 6 μg/♀, y=6.31+6.41x).Conclusion This study established the baseline sensitivity of houseflies through testing the sensitivity of laboratory susceptible strains to six commonly used insecticides, providing a reference for monitoring and assessing the resistance level of houseflies in China.

Key words: Housefly, Topical application, Insecticide, Baseline sensitivity

摘要： 目的该研究旨在建立家蝇对6种常用杀虫剂的敏感基线,为我国家蝇抗药性系统监测和防控提供依据。方法利用点滴法测定本实验室常年饲养的家蝇敏感品系对4大类共6种杀虫剂（溴氰菊酯、高效氯氰菊酯、氯菊酯、敌敌畏、残杀威、呋虫胺）的敏感性,同时收集历史文献中的数据,以半数致死剂量（LD₅₀）的95%置信区间（95%CI）是否重叠来判断敏感性差异有无统计学意义,基于以上方法建立家蝇对这6种杀虫剂的敏感基线。结果供试的6种杀虫剂对家蝇的敏感基线依次为：溴氰菊酯（LD₅₀=0.002 236 μg/♀,y=13.76+5.19x）、高效氯氰菊酯（LD₅₀=0.003 692 μg/♀,y=9.34+3.84x）、氯菊酯（LD₅₀=0.007 152 μg/♀,y=10.03+4.67x）、敌敌畏（LD₅₀=0.074 20 μg/♀,y=5.02+4.44x）、残杀威（LD₅₀=0.415 9 μg/♀,y=0.93+2.43x）、呋虫胺（LD₅₀=0.103 6 μg/♀,y=6.31+6.41x）。结论测定了实验室敏感品系家蝇对6种常用杀虫剂的敏感水平,建立了敏感基线,为我国家蝇抗药性监测和抗性水平评估提供了参考依据。

关键词: 家蝇, 点滴法, 杀虫剂, 敏感基线

CLC Number:

ZHANG Ya-jun, ZHAO Chun-chun, FENG Xing-ming, WANG Jia-lin, LUN Xin-chang, LIU Qi-yong, MENG Feng-xia. Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides[J]. Chinese Journal of Vector Biology and Control, 2023, 34(6): 719-722.

张亚军, 赵春春, 冯星明, 王佳琳, 伦辛畅, 刘起勇, 孟凤霞. 实验室品系家蝇对常用杀虫剂敏感基线的建立[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 719-722.

References

[1] Scott JG. Evolution of resistance to pyrethroid insecticides in Musca domestica[J]. Pest Manag Sci,2017,73（4）:716-722. DOI:10.1002/ps.4328.
[2] Hou YX,Liu ZW,Zhang YJ,et al. Resistance of Musca domestica to six insecticides commonly used in Hefei,China,2021[J]. Chin J Vector Biol Control,2023,34（1）:105-108. DOI:10.11853/j.issn.1003.8280.2023.01.019.（in Chinese）侯银续,刘志伟,张燕婕,等. 2021年合肥市家蝇对6种常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2023,34（1）:105-108. DOI:10.11853/j.issn.1003.8280.2023. 01.019.
[3] Li HL,Li YZ,Wu HY,et al. Introduction on roles of houseflies in transmission of animal infectious diseases[J]. Prog Vet Med,2017,38（2）:107-110. （in Chinese）李好磊,李叶珍,吴浩阳,等. 家蝇在动物疫病传播中的作用研究概况[J]. 动物医学进展,2017,38（2）:107-110.
[4] Scott JG,Leichter CA,Rinkevihc FD,et al. Insecticide resistance in house flies from the United States:Resistance levels and frequency of pyrethroid resistance alleles[J]. Pestic Biochem Physiol,2013,107（3）:377-384. DOI:10.1016/j.pestbp.2013. 10.006.
[5] Wu YY,Liu QM,Wang JN,et al. Resistance of Musca domestica to commonly used insecticides in Zhejiang province,China,2021[J]. Chin J Vector Biol Control,2023,34（1）:26-30. DOI:10.11853/j.issn.1003.8280.2023.01.005.（in Chinese）吴瑜燕,刘钦梅,王金娜,等. 2021年浙江省家蝇对常见卫生杀虫剂的抗药性研究[J]. 中国媒介生物学及控制杂志,2023,34（1）:26-30. DOI:10.11853/j.issn.1003.8280.2023.01.005.
[6] Liu QY, Liu XB, Chang N, et al. Advances and achievements in the surveillance and control of vectors and vector-borne diseases in China,2012-2021[J]. Chin J Vector Biol Control,2022,33（5）:613-621,654. DOI:10.11853/j.issn.1003.8280.2022.05. 001. （in Chinese）刘起勇,刘小波,常楠,等. 2012－2021年我国媒介生物及相关传染病监测控制进展及成效[J]. 中国媒介生物学及控制杂志,2022,33（5）:613-621,654. DOI:10.11853/j.issn.1003.8280. 2022.05.001.
[7] Wang HH,Lyu SL,Zhao R,et al. Establishment of the relative susceptible baselines of Spodoptera frugiperda （Lepidoptera:Noctuidae） larvae to commonly used insecticides[J]. Acta Entomol Sin,2021,64（12）:1427-1432. DOI:10.16380/j.kcxb. 2021.12.008.（in Chinese）王欢欢,吕圣兰,赵瑞,等. 草地贪夜蛾幼虫对常用杀虫剂相对敏感基线的建立[J]. 昆虫学报,2021,64（12）:1427-1432. DOI:10.16380/j.kcxb.2021.12.008.
[8] Zhai GR. Determination of resistance in houseflies and breeding of resistance[J]. Insect Knowl,1982（5）:47-48,42. （in Chinese）翟桂荣. 家蝇抗药性的测定及抗性的培育[J]. 昆虫知识,1982（5）:47-48,42.
[9] Zhang Y,Meng FX,Liu QY,et al. The susceptibility of female adult house fly （Musca domestica） at different eclosion days to deltamethrin[J]. Chin J Vector Biol Control,2005,16（2）:95-97. DOI:10.3969/j.issn.1003-4692.2005.02.005.（in Chinese）张莹,孟凤霞,刘起勇,等. 家蝇雌成虫对溴氰菊酯的敏感性研究[J]. 中国媒介生物学及控制杂志,2005,16（2）:95-97. DOI:10.3969/j.issn.1003-4692.2005.02.005.
[10] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,Standardization Administration of the People’s Republic of China. GB/T 26350-2010 Test methods of fly resistance to insecticides-The bioassay methods forMusca domestica[S]. Beijing:China Standard Press,2011. （in Chinese）中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB/T 26350－2010 蝇类抗药性检测方法家蝇生物测定法[S]. 北京:中国标准出版社,2011.
[11] Liu Y,Zhang SH,Liang ZN,et al. Study on insecticide resistance difference of Aedes albopictus between adjacent sites[J]. Chin J Vector Biol Control,2020,31（4）:490-493. DOI:10.11853/j.issn.1003.8280.2020.04.024.（in Chinese）刘阳,张韶华,梁焯南,等. 相邻场所白纹伊蚊抗药性差异研究[J]. 中国媒介生物学及控制杂志,2020,31（4）:490-493. DOI:10.11853/j.issn.1003.8280.2020.04.024.
[12] Chen DP, Zhou YZ, Qian WP,et al. Study on the insecticide resistance of Musca domestica vicina and control strategies in Sichuan province[J]. Chin J Hyg Insect Equip,2011,17（3）:184-187. （in Chinese）陈东平,周忆昭,钱薇萍,等. 四川省5.12地震灾区家蝇抗药性水平研究[J]. 中华卫生杀虫药械,2011,17（3）:184-187.
[13] Qi YP,Fu XF,Zha YW,et al. Study on resistance of main vectors to commonly used insecticides in Jiaxing,China[J]. Chin J Vector Biol Control,2019,30（3）:337-340. DOI:10.11853/j.issn.1003.8280.2019.03.026.（in Chinese）亓云鹏,富小飞,查亦薇,等. 浙江省嘉兴市主要病媒生物对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2019,30（3）:337-340. DOI:10.11853/j.issn.1003.8280. 2019.03.026.
[14] Liu KH,Lyu GB,Jin ZJ,et al. Resistance of Musca domestica to 5 pesticides in Handan city[J]. J Med Pest Control,2015,31（12）:1381-1382,1385. DOI:10.7629/yxdwfz201512028.（in Chinese）刘凯华,吕广波,靳增军,等. 邯郸市家蝇对5种杀虫剂的抗药性研究[J]. 医学动物防制,2015,31（12）:1381-1382,1385. DOI:10.7629/yxdwfz201512028.
[15] Lyu W,Huo LX,Sun YX. Investigation of resistance of Musca domestica to insecticides in the four areas of Shaanxi province [J]. Chin J Vector Biol Control,2012,23（4）:314-316. （in Chinese）吕文,霍丽霞,孙养信. 陕西省不同地区家蝇抗药性调查[J]. 中国媒介生物学及控制杂志,2012,23（4）:314-316.
[16] Lu YH,Yang T,Gao XW. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi （Linnaeus） and Sitobion avenae （Fabricius）（Homoptera:Aphididae） by the method of residual film in glass tube [J]. Acta Entomol Sin,2009,52（1）:52-58. DOI:10.3321/j.issn:0454-6296.2009.01.008.（in Chinese）鲁艳辉,杨婷,高希武. 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立[J]. 昆虫学报,2009,52（1）:52-58. DOI:10.3321/j.issn:0454-6296.2009.01.008.
[17] Khan HAA. Characterization of permethrin resistance in a Musca domestica strain:Resistance development,cross-resistance potential and realized heritability [J]. Pest Manag Sci,2019,75（11）:2969-2974. DOI:10.1002/ps.5409.
[18] Shan C,Zhang Y,Ma Z,et al. Inheritance of propoxur resistance in a near-isogenic line of Musca domestica （Diptera:Muscidae） [J]. J Econ Entomol, 2016,109（2）:873-878. DOI:10.1093/jee/tow001.
[19] Liu ZQ,Yuan LF,Liao XJ,et al. Research progress of neonicotinoid insecticide dinotefuran[J]. Mod Agrochem,2021,20（1）:7-12. DOI:10.3969/j.issn.1671-5284.2021.01.002.（in Chinese）刘子琪,袁龙飞,廖先骏,等. 新烟碱类杀虫剂呋虫胺的研究进展[J]. 现代农药,2021,20（1）:7-12. DOI:10.3969/j.issn.1671-5284.2021.01.002.