Investigation of Babesia bigemina carried by ticks in Shibing county,  Guizhou province,  China

doi:10.11853/j.issn.1003.8280.2023.02.019

Chinese Journal of Vector Biology and Control ›› 2023, Vol. 34 ›› Issue (2): 254-261.DOI: 10.11853/j.issn.1003.8280.2023.02.019

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Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China

WU Sheng-chun¹, MENG Jiao¹, HUANG Jian-sheng², YU Fu-xun³, WU Jia-hong¹, YANG Guang-hong⁴, JIANG Jia-fu⁵, SUN Yi⁵, CAO Wu-chun⁵, ZHAN Lin^1,3

1. School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
2. Medical School of Guizhou University, Guiyang, Guizhou 550025, China;
3. Central Laboratory, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China;
4. Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550001, China;
5. Academy of Military Medicine, Academy of Military Sciences, Beijing 100001, China

Received:2022-10-26 Online:2023-04-20 Published:2023-04-26
Supported by:
National Natural Science Foundation of China (No. 82160633,81760605);High-level and Innovative Talents of Guizhou Province (No. QKH-GCC[2022]033-1)

贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究

吴胜春¹, 孟娇¹, 黄健胜², 余福勋³, 吴家红¹, 杨光红⁴, 江佳富⁵, 孙毅⁵, 曹务春⁵, 詹琳^1,3

1. 贵州医科大学公共卫生与健康学院, 环境污染与疾病监控教育部重点实验室, 贵州贵阳 550025;
2. 贵州大学医学院, 贵州贵阳 550025;
3. 贵州省人民医院中心实验室, 贵州贵阳 550002;
4. 贵州省疾病预防控制中心, 贵州贵阳 550001;
5. 军事科学院军事医学研究院, 北京 100001

通讯作者: 詹琳,E-mail：zhanlin300@hotmail.com
作者简介:吴胜春,女,苗族,在读硕士,主要从事蜱媒病分子流行病学研究,E-mail：2935769738@qq.com
基金资助:
国家自然科学基金（82160633，81760605）；贵州省高层次创新型人才（黔科合平台人才-GCC〔2022〕033-1）

Abstract

Abstract: Objective To investigate the infection of Babesia bigemina in ticks at Shibing county in Guizhou province of China. Methods Ticks were collected from the body surface of free-range cattle in Shibing from 2021 to 2022. The 16S rDNA and cytochrome c oxidase subunit Ⅰ(COX1) gene detection techniques were used to identify the tick species after preliminary morphological classification. The samples were initially screened with Piroplasma 18S rDNA gene, followed by PCR verification and sequencing with B. bigemina apical membrane antigen-1 (AMA-1) gene. The resulting sequences were aligned using BLAST at the National Center for Biotechnology Information (NCBI) website. Phylogenetic trees were constructed by using the neighbor-joining method to understand the genetic evolution characteristics of tick-borne B. bigemina in Shibing. Results In this study, 615 ticks were collected and assigned to 2 genera and 4 species, including Rhipicephalus microplus, R. haemaphysaloides, Haemaphysalis longicornis, and H. kitaokai. Nucleic acid fragments of B. bigemina were detected in R. microplus. The sequencing result showed that the AMA-1 gene sequence was closely related to the B. bigemina detected from bovine blood in the Philippines and the tick of Hyalomma anatolicum in Sudan, and the 18S rDNA sequence was most closely related to B. bigemina in India. Conclusions The parasitic ticks on the body surface of free-range cattle in Shibing carry the nucleic acid fragments of B. bigemina, suggesting infection of the vector ticks in Shibing with B. bigemina. Our results provide reference data for the prevention and control of tick-borne protozoonosis in this area.

Key words: Parasitic tick, Piroplasma, Babesia bigemina, Guizhou, Shibing

摘要： 目的了解贵州省施秉县蜱的双芽巴贝斯虫感染情况。方法于2021－2022年在贵州省施秉县采集散养黄牛体表寄生蜱，经形态学分类后辅以16S rDNA及细胞色素C氧化酶亚基Ⅰ（COX1）基因检测鉴定蜱种类；用梨形虫18S rDNA对样本进行初筛，后用双芽巴贝斯虫顶端膜抗原1（AMA-1）基因进行PCR验证及序列测定，所测序列通过美国国立生物技术信息中心（NCBI）的BLAST进行比对，采用邻接法构建进化树，掌握施秉县蜱携带双芽巴贝斯虫的遗传进化特征。结果共采集蜱样本615只，分子生物学鉴定有2属4种，分别为微小扇头蜱、镰形扇头蜱、长角血蜱和北岗血蜱；从微小扇头蜱体内检测到双芽巴贝斯虫核酸片段，结果表明，AMA-1基因序列与从菲律宾的牛血及苏丹的小亚璃眼蜱中检出的双芽巴贝斯虫有较近的亲缘关系，18S rDNA基因序列与印度的双芽巴贝斯虫亲缘关系最近。结论施秉县散养家畜体表寄生蜱体内携带双芽巴贝斯虫的核酸片段，提示施秉县媒介蜱可能存在巴贝斯虫感染。研究结果可为该地区开展蜱传原虫病防治工作提供参考数据。

关键词: 寄生蜱, 梨形虫, 双芽巴贝斯虫, 贵州, 施秉

CLC Number:

WU Sheng-chun, MENG Jiao, HUANG Jian-sheng, YU Fu-xun, WU Jia-hong, YANG Guang-hong, JIANG Jia-fu, SUN Yi, CAO Wu-chun, ZHAN Lin. Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(2): 254-261.

吴胜春, 孟娇, 黄健胜, 余福勋, 吴家红, 杨光红, 江佳富, 孙毅, 曹务春, 詹琳. 贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 254-261.

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Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China

贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究

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