Rickettsia carried by gamasid mites on the body surface of rodents in Inner Mongolia,  China

doi:10.11853/j.issn.1003.8280.2023.02.017

Abstract

Abstract: Objective To determine the species and positive rate of Rickettsia carried by gamasid mites on the body surface of rodents in Inner Mongolia Autonomous Region (Inner Mongolia), China, and to provide a basis for the prevention and control of local infectious diseases. Methods DNAs of gamasid mites on the body surface of rodents in different areas of Inner Mongolia were extracted, and the 17 kDa gene sequence was amplified by nested polymerase chain reaction (PCR). The outer membrane protein A (ompA) sequence was amplified for samples positive for the 17 kDa gene. The PCR products obtained by amplification were sequenced, and the phylogenetic tree was constructed after homology comparison between the sequencing results and the sequences from the National Center for Biotechnology Information, USA. Results Rickettsia was detected in 11 of 536 gamasid mites, and the total positive rate was 2.05%. The species of Rickettsia included R. heilongjiangensis, a sibling species of R. felis, and an unknown species of Rickettsia. Their host mites included Haemogamasus mandschuricus, Eulaelaps cricetuli, Hg. kitanoi, Haemolaelaps glasgowi, and deutonymphs of Parasitus sp., with the positive rates of 8.89%, 3.39%, 2.22%, 0.68%, and 12.50%, respectively. The hosts that carried gamasid mites positive for Rickettsia included Spermophilus dauricus, Allactaga sibirica, and Lasiopodomys brandtii. Conclusion Gamasid mites on the body surface of rodents in Inner Mongolia carry a variety of Rickettsia, including the species that are human pathogen.

Key words: Gamasid mites, Rickettsia, 17 kDa protein, Outer membrane protein A(ompA), Inner Mongolia

摘要： 目的通过检测内蒙古自治区（内蒙古）鼠体革螨携带立克次体种类和阳性率，为当地相关传染病防控提供依据。方法对内蒙古不同地区鼠类体表革螨提取DNA，用巢式PCR扩增17 kDa基因序列，对17 kDa阳性样本再进行外膜蛋白A（ompA）序列扩增。对扩增所得PCR产物进行测序，测序结果与美国国立生物技术信息中心（NCBI）中序列进行同源性比对后构建系统发育树。结果在536只革螨中，有11只革螨检出立克次体，总阳性率为2.05%，包括黑龙江立克次体、猫立克次体近缘种和1种未知立克次体。其宿主包括东北血革螨、仓鼠真厉螨、北野血革螨、格氏血厉螨以及1种寄螨属种类的第二若螨，阳性率分别为8.89%、3.39%、2.22%、0.68%和12.50%。阳性革螨宿主包括达乌尔黄鼠、五趾跳鼠和布氏田鼠。结论内蒙古地区鼠体革螨携带多种立克次体，其中包括对人类致病的种类。

关键词: 革螨, 立克次体, 17 kDa蛋白, 外膜蛋白A, 内蒙古

CLC Number:

R376
R384.4

WANG Bin, LI Gui-chang, DONG Li, MU Qun-zheng, ZHAO Ning, SONG Xiu-ping, LU Liang, LI Dong-mei, LI Xing-zhou, LIU Qi-yong. Rickettsia carried by gamasid mites on the body surface of rodents in Inner Mongolia, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(2): 244-249.

王彬, 李贵昌, 董利, 母群征, 赵宁, 宋秀平, 鲁亮, 栗冬梅, 李兴洲, 刘起勇. 内蒙古地区鼠类体表革螨携带立克次体研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 244-249.

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Rickettsia carried by gamasid mites on the body surface of rodents in Inner Mongolia, China

内蒙古地区鼠类体表革螨携带立克次体研究

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