A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping,  Beijing,  China

doi:10.11853/j.issn.1003.8280.2023.02.009

Abstract

Abstract: Objective To perform bioinformatics analysis of differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Aedes albopictus population in Changping, Beijing, China, to study the metabolic mechanism of beta-cypermethrin resistance in Ae. albopictus, and to provide theoretical a basis for the rational use of insecticides according to the drug resistance of Ae. albopictus. Methods Beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus population in Changping were selected by biological assay. Total proteins were extracted for LC-MS/MS analysis. Differentially expressed proteins were identified by bioinformatics analysis and confirmed at the mRNA level. Results A total of 1 861 proteins were identified by bioinformatics analysis, of which 383 were differentially expressed, including 213 up-regulated and 170 down-regulated proteins in resistant individuals. Eight differentially expressed proteins related to drug metabolism according to KEGG pathway enrichment analysis (P<0.05) were selected for validation at the mRNA level. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase were up-regulated in resistant individuals. Conclusions There are differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase are up-regulated in resistant individuals, which may be associated with beta-cypermethrin resistance.

Key words: Aedes albopictus, Insecticide resistance, Comparative proteomics

摘要： 目的对白纹伊蚊昌平种群高效氯氰菊酯抗性个体与敏感个体之间的差异表达蛋白进行生物信息学分析，研究白纹伊蚊高效氯氰菊酯代谢抗性机制，为白纹伊蚊抗药性以及杀虫剂的合理使用提供理论依据。方法通过生物学测定筛选出白纹伊蚊昌平种群高效氯氰菊酯抗性个体与敏感个体，提取总蛋白进行液相色谱串联质谱（LC-MS/MS）分析，通过生物信息学分析找到差异表达蛋白，并在mRNA水平上进行验证。结果生物信息学分析共鉴定到1 861个蛋白，其中差异表达蛋白有383个，在抗性个体体内上调表达的有213个，下调表达的170个。选择京都基因与基因组百科全书（KEGG）通路富集分析中显著性P<0.05的功能通路中与药物代谢相关的8个差异表达蛋白进行验证，鉴定到丙氨酸氨基转移酶、尿苷酸-胞苷酸（UMP-CMP）激酶、谷胱甘肽S-转移酶（GST）在抗性个体体内上调表达。结论白纹伊蚊抗性个体与敏感个体之间存在差异表达蛋白，其中丙氨酸氨基转移酶、UMP-CMP激酶、GST在抗性群体中上调表达，可能与高效氯氰菊酯代谢抗性相关。

关键词: 白纹伊蚊, 杀虫剂抗性, 比较蛋白质组学

CLC Number:

HUA Dong-dong, WANG Lei, XIAO Di, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MU Qun-zheng, LIU Qi-yong, MA Wei, MENG Feng-xia. A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(2): 196-203.

华栋栋, 王磊, 肖迪, 李文玉, 周欣欣, 伦辛畅, 母群征, 刘起勇, 马伟, 孟凤霞. 白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 196-203.

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A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China

白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究

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