Effect of Aeromonas hydrophila on the transcriptome and metabolome of Culex pipiens pallens

doi:10.11853/j.issn.1003.8280.2023.02.008

Abstract

Abstract: Objective To observe the effect of feeding Aeromonas hydrophila on the transcriptome and metabolome of adult Culex pipiens pallens, and to further study the function of A. hydrophila. Methods Transcriptome and metabolome sequencing were performed on adult Cx. pipiens pallens fed with A. hydrophila and its control group, and a conjoint analysis was performed on the results of transcriptome and metabolome. Results A total of 305 differentially expressed genes were identified in the transcriptome. Functional enrichment analysis showed that the genes were mainly glucose metabolism, lipid metabolism, and protein metabolism pathways. A total of 575 differential metabolites were obtained in the metabolome, and functional enrichment analysis showed that the main metabolites were citric acid cycle and amino acid metabolism pathway. Conjoint analysis of the transcriptome and metabolome revealed that five pathways were co-enriched, which were glycolysis/gluconeogenesis, glycerophospholipid metabolism, citric acid cycle, alanine, aspartic and glutamic acid metabolism, and pyruvate metabolism. The enrichment of citric acid cycle had the highest significance. Conclusions A. hydrophila can activate the glucose metabolism, lipid metabolism, and amino acid metabolism pathways of Cx. pipiens pallens, and provide essential energy, protein, and lipid for Cx. pipiens pallens.

Key words: Culex pipiens pallens, Aeromonas hydrophila, Transcriptome, Metabolome, Conjoint analysis

摘要： 目的观察喂饲嗜水气单胞菌对淡色库蚊成蚊转录组与代谢组的影响，进一步研究嗜水气单胞菌的功能。方法分别对喂饲嗜水气单胞菌的淡色库蚊成蚊及其对照组进行转录组与代谢组测序，并对转录组与代谢组结果进行联合分析。结果通过筛选，在转录组中获得了305个差异表达基因，功能富集分析显示主要为糖代谢、脂代谢和蛋白质代谢通路；在代谢组中共获得575个差异代谢物，功能富集分析显示主要为柠檬酸循环和氨基酸代谢通路；转录组与代谢组联合分析共同富集的通路有5个，分别为糖酵解/糖异生，甘油磷脂代谢，柠檬酸循环，丙氨酸、天冬氨酸和谷氨酸代谢，丙酮酸代谢。其中柠檬酸循环富集的显著性最高。结论嗜水气单胞菌可激活淡色库蚊的糖代谢、脂代谢、氨基酸代谢通路，为淡色库蚊提供必要的能量、蛋白质和脂质。

关键词: 淡色库蚊, 嗜水气单胞菌, 转录组, 代谢组, 联合分析

CLC Number:

R384.1

LYU Wen-xiang, CHENG Peng, LEI Jing-jing, GUO Xiu-xia, WANG Hai-fang, GONG Mao-qing, LIU Li-juan. Effect of Aeromonas hydrophila on the transcriptome and metabolome of Culex pipiens pallens[J]. Chinese Journal of Vector Biology and Control, 2023, 34(2): 189-195.

吕文祥, 程鹏, 类晶晶, 郭秀霞, 王海防, 公茂庆, 刘丽娟. 嗜水气单胞菌对淡色库蚊转录组和代谢组的影响[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 189-195.

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