Responses of arginine kinase genes CpAK1 and CpAK2 in Culex pipiens pallens to adversity stresses

doi:10.11853/j.issn.1003.8280.2022.06.003

Abstract

Abstract: Objective To investigate the response characteristics of arginine kinase genes CpAK1 and CpAK2 of Culex pipiens pallens to temperature and humidity stresses. Methods Real-time quantitative PCR and Western blot were used to determine the mRNA and protein levels in adult Cx. pipiens pallens under 38 ℃/4 ℃ and 100%/20% relative humidity. Results CpAK1 mRNA level was up-regulated 3.46 and 3.53 folds at 38 ℃ and 4 ℃, respectively, and peaked at 6 h at both temperatures. CpAK2 mRNA level was statistically down-regulated at 38 ℃ and 4 ℃ (P<0.05). The protein levels of CpAK1 and CpAK2 showed similar trends to their mRNA levels at 38 ℃ and 4 ℃. At 20% relative humidity, CpAK1 mRNA expression was statistically up-regulated 4.30 folds at 6 h (P<0.05), while CpAK2 mRNA level was down-regulated to 45.45% at 12 h. At 100% relative humidity, CpAK1 mRNA expression showed no significant change, while CpAK2 mRNA level was statistically down-regulated (P<0.01). The protein levels of CpAK1 and CpAK2 showed similar trends to their mRNA expression under different temperature and humidity stresses. Conclusion The mRNA and protein levels of CpAK1 and CpAK2 vary significantly under different temperature and humidity stresses. In general, CpAK1 is up-regulated, while CpAK2 is down-regulated. It is speculated that CpAK1 and CpAK2 have different functions in the stress response of Cx. pipiens pallens.

Key words: Culex pipiens pallens, Arginine kinase, Adversity stress, mRNA level, Protein level

摘要： 目的了解淡色库蚊精氨酸激酶基因CpAK1和CpAK2对温度、湿度逆境胁迫的响应特性。方法采用实时荧光定量PCR（RT-qPCR）法和蛋白免疫印迹法，分别检测淡色库蚊成蚊在38、4 ℃，相对湿度100%、20%胁迫下CpAK1和CpAK2的mRNA及蛋白水平。结果在38和4 ℃胁迫下，CpAK1的mRNA水平整体升高，均在6 h达到峰值，分别上调3.46和3.53倍。而CpAK2的mRNA水平显著下调，差异有统计学意义（P<0.05）。38和4 ℃胁迫下CpAK1和CpAK2蛋白水平与mRNA水平变化基本一致。在相对湿度20%胁迫下，CpAK1的mRNA水平在6 h显著上调了4.30倍，差异有统计学意义（P<0.05），CpAK2的mRNA水平在12 h下调至45.45%。在相对湿度100%环境下，CpAK1的mRNA水平无明显变化，而CpAK2的mRNA水平显著下调，差异有统计学意义（P<0.01）。不同温度和湿度胁迫下CpAK1和CpAK2的蛋白水平与mRNA水平变化基本一致。结论不同温度、湿度胁迫下，CpAK1和CpAK2的mRNA水平和蛋白水平发生明显变化，总体趋势是CpAK1上调而CpAK2下调，推测CpAK1和CpAK2在淡色库蚊应对逆境胁迫时发挥着不同功能。

关键词: 淡色库蚊, 精氨酸激酶, 逆境胁迫, mRNA水平, 蛋白水平

CLC Number:

R384.1

GUAN Qing-qing, DAI Yu-qi, QIN Sheng-feng, QIAN Kun. Responses of arginine kinase genes CpAK1 and CpAK2 in Culex pipiens pallens to adversity stresses[J]. Chinese Journal of Vector Biology and Control, 2022, 33(6): 781-786.

关晴晴, 戴雨琪, 覃盛锋, 钱坤. 淡色库蚊精氨酸激酶基因CpAK1和CpAK2对逆境胁迫的响应[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 781-786.

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