Establishment of beta-cypermethrin-resistant strains of Musca domestica in Hangzhou

doi:10.11853/j.issn.1003.8280.2022.04.002

Abstract

Abstract: Objective To screen and purify stable strains highly resistant to beta-cypermethrin using the field samples of Musca domestica in Hangzhou, China. Methods Houseflies were collected from different locations in Hangzhou, and adult houseflies were screened by direct spray with beta-cypermethrin. After screening for 5 generations, the houseflies were maintained for two years. Resistance was determined using topical application method for F2 against deltamethrin, beta-cypermethrin, dichlorvos, triclosan, and propoxur, for F7 against deltamethrin and beta-cypermethrin, and for houseflies maintained for 1 and 2 years against deltamethrin and beta-cypermethrin. The resistance data of sensitive strains were provided by the department of infectious diseases, Zhejiang provincial center for disease control and prevention. Results The F2 generation of M. domestica in Hangzhou developed resistance to the five commonly used insecticides. The LD₅₀of propoxur was >400.000 0 μg/♀, with the highest resistance and R/S >1 345.00. Resistance to other insecticide showed R/S between 6.79 and 77.33. The R/Sfor deltamethrin was 77.33 (LD₅₀=0.069 6μg/♀) and the R/S for beta-cypermethrin was 55.58 (LD₅₀=0.200 1 μg/♀). After being screened with beta-cypermethrin for 5 consecutive generations, compared with F2, F7 showed 29.89% and 51.27% increases in resistance to deltamethrin and beta-cypermethrin, respectively. After 2 years of normal rearing, the beta-cypermethrin-resistant strain showed decreased resistance as compared with the F7 generation; however, compared with sensitive strains, the R/S values for deltamethrin and beta-cypermethrin were 70.22 and 56.25, respectively, and the resistance remained at high levels. The LD₅₀ for propoxur remained above 400.000 0 μg/♀. Conclusion After being screened with beta-cypermethrin for 5 generations and maintaining for two years, relatively stable beta-cypermethrin-resistant strains of M. domestica in Hangzhou were obtained, which lays a foundation for further research on the resistance mechanism of M. domestica.

Key words: Musca domestica, Insecticide resistance, Insecticide

摘要： 目的对杭州市家蝇野外种群进行高效氯氰菊酯高抗稳定品系的筛选与纯化。方法采集杭州市不同方位的家蝇，用高效氯氰菊酯直接喷雾法筛选家蝇成蝇，选育5代后进行维持饲养2年。采用微量点滴法测定家蝇F2代对溴氰菊酯、高效氯氰菊酯、敌敌畏、三氯杀虫酯、残杀威的抗药性；测定家蝇F7代对溴氰菊酯、高效氯氰菊酯的抗药性；测定家蝇维持饲养1年与2年时对溴氰菊酯、高效氯氰菊酯的抗药性。敏感品系抗药性测试数据由浙江省疾病预防控制中心传染病预防控制所病媒生物防制科提供。结果杭州市家蝇野外种群F2代（雌蝇）对5种常用杀虫剂均已产生抗性，残杀威的LD₅₀ >400.000 0 μg/只，抗性倍数>1 345.00，抗性水平最高，对其他杀虫剂的抗性倍数在6.79～77.33，其中对溴氰菊酯的抗性为77.33倍（LD₅₀=0.069 6 μg/只），对高效氯氰菊酯抗性为55.58倍（LD₅₀=0.200 1 μg/只）。进一步用高效氯氰菊酯连续5代筛选后，F7代与F2代相比，对溴氰菊酯和高效氯氰菊酯的抗性分别升高29.89%和51.27%。经过2年正常饲养，杭州市家蝇高效氯氰菊酯抗性品系抗药性表型较F7代有一定下降，但与敏感品系相比，对溴氰菊酯和高效氯氰菊酯的抗性分别为70.22和56.25倍，仍处于高抗水平。整个筛选过程中残杀威始终LD₅₀>400.000 0 μg/只。结论经过高效氯氰菊酯连续5代的筛选和2年的维持饲养，获得较为稳定的杭州市家蝇高效氯氰菊酯抗性品系，为进一步研究家蝇的抗药性机制奠定了基础。

关键词: 家蝇, 抗药性, 杀虫剂

CLC Number:

WEI Ling-ya, KONG Qing-xin, CAO Yang, WANG Hui-min, SHEN Lin-hai, WANG Ying-hong. Establishment of beta-cypermethrin-resistant strains of Musca domestica in Hangzhou[J]. Chinese Journal of Vector Biology and Control, 2022, 33(4): 458-461.

韦凌娅, 孔庆鑫, 曹阳, 王慧敏, 沈林海, 王英红. 杭州市家蝇高效氯氰菊酯抗性品系的建立[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 458-461.

References

[1] Zhu F, Lavine L, O'Neal S, et al. Insecticide resistance and management strategies in urban ecosystems[J]. Insects, 2016, 7(1):2. DOI:10.3390/insects7010002.
[2] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 26350-2010蝇类抗药性检测方法家蝇生物测定法[S]. 北京:中国标准出版社, 2011. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China. GB/T 26350-2010 Test methods of fly resistance to insecticides-The bioassay methods for Musca domestica[S]. Beijing:Standards Press of China, 2011. (in Chinese)
[3] 耿晓飞, 李超, 高志鹏, 等. 北京市怀柔区2014和2016年家蝇对常用化学杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2018, 29(4):388-390. DOI:10.11853/j.issn.1003.8280. 2018.04.017. Geng XF, Li C, Gao ZP, et al. Resistance of Musca domestica to commonly used insecticides in Huairou district in Beijing, during 2014 and 2016[J]. Chin J Vector Biol Control, 2018, 29(4):388-390. DOI:10.11853/j.issn.1003.8280.2018.04.017.(in Chinese)
[4] 涂涛田, 何亚明, 冯绍全, 等. 贵州省盘县家蝇抗药性调查及防制策略分析[J]. 中国热带医学, 2019, 19(5):492-493. DOI:10.13604/j.cnki.46-1064/r.2019.05.22. Tu TT, He YM, Feng SQ, et al. Resistance of Musca domestica to commonly used insecticides and its countermeasures in Pan county of Guizhou[J]. China Trop Med, 2019, 19(5):492-493. DOI:10.13604/j.cnki.46-1064/r.2019.05.22.(in Chinese)
[5] 施红喜, 李培珍, 何健. 金华市家蝇抗药性水平趋势分析[J]. 实用预防医学, 2020, 27(1):115-116. DOI:10.3969/j.issn. 1006-3110.2020.01.034. Shi HX, Li PZ, He J. Trend analysis of insecticide resistance of Musca domestica in Jinhua city[J]. Pract Prev Med, 2020, 27(1):115-116. DOI:10.3969/j.issn.1006-3110.2020.01.034.(in Chinese)
[6] 戈斌, 李星成, 刘清, 等. 上海市奉贤区家蝇对5种常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2020, 26(3):292-293. DOI:10.19821/j.1671-2781.2020.03.029. Ge B, Li XC, Liu Q, et al. Resistance of Musca domestica to five commonly used insecticides in Fengxian district of Shanghai[J]. Chin J Hyg Insect Equip, 2020, 26(3):292-293. DOI:10.19821/j.1671-2781.2020.03.029.(in Chinese)
[7] 王唐, 宋灿磊, 李淑华, 等. 上海市金山区家蝇对5种杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2021, 27(4):382-383. DOI:10.19821/j.1671-2781.2021.04.024. Wang T, Song CL, Li SH, et al. Resistance of Musca domestica to five insecticides in Jinshan district of Shanghai[J]. Chin J Hyg Insect Equip, 2021, 27(4):382-383. DOI:10.19821/j.1671-2781.2021.04.024.(in Chinese)
[8] 张咏梅, 王源, 王姝, 等. 天津市家蝇对4种常用杀虫剂的抗性调查[J]. 职业与健康, 2021, 37(13):1811-1814. DOI:10.13329/j.cnki.zyyjk.2021.0431. Zhang YM, Wang Y, Wang S, et al. An investigation of resistance of Musca domestica to four commonly used insecticides in Tianjin[J]. Occup Health, 2021, 37(13):1811-1814. DOI:10.13329/j.cnki.zyyjk.2021.0431.(in Chinese)
[9] 陈晓敏, 刘芹, 周良才, 等. 武汉市2009-2015年家蝇对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2021, 32(4):468-471. DOI:10.11853/j.issn.1003.8280.2021.04.017. Chen XM, Liu Q, Zhou LC, et al. An investigation of resistance of Musca domestica to commonly used insecticides in Wuhan, China, 2009-2015[J]. Chin J Vector Biol Control, 2021, 32(4):468-471. DOI:10.11853/j.issn.1003.8280.2021.04.017.(in Chinese)
[10] 阎利敏, 师佳佳, 张月泉, 等. 郑州市区淡色库蚊和家蝇对常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2020, 26(3):220-222. DOI:10.19821/j.1671-2781.2020.03.009. Yan LM, Shi JJ, Zhang YQ, et al. Resistance of Culex pipiens pallens and Musca domestica in Zhengzhou to commonly used insecticides[J]. Chin J Hyg Insect Equip, 2020, 26(3):220-222. DOI:10.19821/j.1671-2781.2020.03.009.(in Chinese)
[11] 曹辉, 霍新北, 王学军. 家蝇对高效氯氰菊酯抗药性的选育研究[J]. 中国媒介生物学及控制杂志, 2010, 21(1):56-58. Cao H, Huo XB, Wang XJ. Study of the resistance of Musca domestica against beta-cypermethrin through selective breeding[J]. Chin J Vector Biol Control, 2010, 21(1):56-58. (in Chinese)
[12] 张莹, 唐勇, 孙炳欣. 家蝇对高效氯氰菊酯抗药性的选育研究[J]. 中华卫生杀虫药械, 2018, 24(1):27-28. DOI:10.19821/j.1671-2781.2018.01.009. Zhang Y, Tang Y, Sun BX. Selection of resistant strain of Musca domestica against beta-cypermethrin[J]. Chin J Hyg Insect Equip, 2018, 24(1):27-28. DOI:10.19821/j.1671-2781.2018. 01.009.(in Chinese)
[13] 王志钢, 海秀平, 许永红, 等. 家蝇对两种拟除虫菊酯类杀虫剂抗性的稳定性[J]. 中国媒介生物学及控制杂志, 2006, 17(4):286-287. DOI:10.3969/j.issn.1003-4692.2006.04.009. Wang ZG, Hai XP, Xu YH, et al. Stability of two kinds of pyrethroids resistance in housefly (Diptera:Muscidae)[J]. Chin J Vector Biol Control, 2006, 17(4):286-287. DOI:10.3969/j.issn.1003-4692.2006.04.009.(in Chinese)
[14] 王学军, 霍新北, 宫学诗, 等. 家蝇对化学杀虫剂抗药性及抗性自然衰减趋势[J]. 中国公共卫生, 2008, 24(11):1330-1331. DOI:10.3321/j.issn:1001-0580.2008.11.033. Wang XJ, Huo XB, Gong XS, et al. The resistance of housefly to chemical insecticides and their natural attenuation trend[J]. Chin J Public Health, 2008, 24(11):1330-1331. DOI:10.3321/j.issn:1001-0580.2008.11.033.(in Chinese)

[1]	LI Tian-qi, WU Yu-yan, LIU Qin-mei, LUO Ming-yu, WANG Jin-na, GONG Zhen-yu. Surveillance results of the insecticide resistance of Blattella germanica in Zhejiang province of China, 2018 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(4): 462-465.
[2]	MA Zhe, ZHENG Yu-ting, YANG Rui, XU Xiang, WEI Chun, YU Xiao-jun, YANG Chun-mei, JIANG Jin-yong. A study of population density, breeding habitat, and insecticide resistance of Aedes albopictus in Simao district, Pu'er, Yunnan province, China, 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(3): 360-365.
[3]	LIN Zu-rui, LIN Ying-kun, GUO Xiang-rui, YANG Jie, LI Shi-gang, DONG Chao-liang, CHEN Li-fei, ZHOU Xian-hua, GUAN Gui-qin, SUN Xiao-dong. Resistance of Anopheles sinensis to five insecticides in China-Myanmar border areas in Yingjiang county, Yunnan province, China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(3): 430-433.
[4]	GAO Yang-ye, WU Ying, DU Guo-jun, HAN Hui-qiang. An investigation of resistance of Musca domestica to commonly used public health insecticides in Chengde, Hebei province, China, 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(3): 434-437.
[5]	LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MA De-long, LIN Ling-hong, SONG Xiu-ping, LU Liang, LIU Qi-yong, WANG Jun, WANG Chun-lan, MENG Feng-xia. A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides [J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 171-176.
[6]	WU Si-han, ZHOU Xin-xin, KE Xue-mei, LI Yu-wei, CHEN Bin, HUANG Jing-wen, CHEN Hua-fang, LIN Min-zhen, LEI Lei, GUO Zhi-nan, MENG Feng-xia. Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 177-182.
[7]	WANG You-jun, ZHANG Xue-tai, WU Jia-hui. Resistance testing of Musca domestica to five insecticides in Baiyin city of Gansu province, China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 234-238.
[8]	ZHAO Chun-chun, ZHOU Xin-xin, LI Wen-yu, LUN Xin-chang, LIU Xiao-bo, WU Hai-xia, WANG Jun, LIU Qi-yong, MENG Feng-xia. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 30-37.
[9]	ZHENG Yu-ting, YANG Chun-mei, YANG Ming-dong, JIANG Jin-yong. Ecological and insecticide resistance surveillance of dengue vector Aedes in Yunnan border region of China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 38-43.
[10]	TU Tao-tian, XIAO Han-sen, MENG Feng-xia, LIU Qi-yong, LIU Xiao-bo, HE Ya-ming, JI Heng-qing. An investigation of the insecticide resistance of Aedes vector after emergency control of a dengue fever outbreak in Chongqing， China， 2019 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 44-47.
[11]	LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 104-107.
[12]	ZHAO Qiong-yao, QIU Xing-hui, YANG Yan, LUO Xing, SONG Xiao-ming, JIA Yong-chao. Polymorphism and resistance mutation frequency of the target genes of insecticides in Anopheles sinensis in Guangyuan of Sichuan province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(5): 541-545.
[13]	HU Yuan-feng, TAN Liang-fei. Surveillance results of cockroach density and insecticide resistance in Ezhou of Hubei province, China, 2016-2020 [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 599-603.
[14]	CHEN Xiao-min, LIU Qin, ZHOU Liang-cai, WU Li-qun, BAO Ji-yong, WU Tai-ping. An investigation of resistance of Musca domestica to commonly used insecticides in Wuhan, China, 2009-2015 [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 468-471.
[15]	LI Feng, CUI Shi-lei, YAN Jing, DING Xin-yang. An investigation of the resistance of Blattella germanica to commonly used insecticides in Nanyang, Henan province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 472-474.