Abstract: Objective To investigate the effects of different diets on the density of Wolbachia in the ovary, fat body, and the other tissues in female Aedes aegypti of the WB strain. Methods After eclosion, Ae. aegypti mosquitoes of the WB strain were divided into glucose group (fed with glucose), white sugar group (fed with white sugar), and blood group (fed with blood for 2 hours on the 3rd day). Mosquitoes in each group were collected on days 4, 4.5, 5, and 6 to harvest the ovary, fat body, and the other tissues. DNA was extracted to determine the density of Wolbachia in all tissues using quantitative real-time PCR. SPSS 20.0 software was used for data analysis. The Kruskal-Wallis H test was used to compare the density of Wolbachia under different diets and at different days of age.Results On day 4.5, Wolbachia densities in the ovary in the glucose, white sugar, and blood groups were 2.149, 2.773, and 0.761, respectively, with a statistically lower density in the blood group (H=40.754, P<0.001); Wolbachia densities in the fat body in the three groups were 0.859, 1.189, 1.298, respectively, and the differences were not statistically significant (H=1.631, P=0.442). Wolbachia densities in the other tissues were 0.505, 0.405, 1.012, respectively, and the differences were not statistically significant (H=6.306, P=0.043). Under different diets but at the same days of age, Wolbachia densities in the ovary were lowest in the blood group on days 4, 5, and 6, and highest in the white sugar group on days 4 and 6 (H=14.335, P=0.001; H=22.049, P<0.001; H=4.266, P=0.084); for the fat body on days 4 and 6 and the other tissues on days 4, 5, and 6, Wolbachia densities were highest in the blood group, followed by the white sugar group, and lowest in the glucose group (H=7.186, P=0.028; H=10.504, P=0.005; H=16.338, P<0.001; H=14.083, P=0.001; H=4.266, P=0.118). At different days of age but under the same diets, Wolbachia densities in the ovary, fat body, and the other tissues in the glucose group had no statistical differences between different days of age (H=4.683, P=0.096; H=2.451, P=0.294; H=0.293, P=0.864); under the white sugar diet, Wolbachia densities in the ovary and fat body were no statistically increased with days of age (H=4.731, P=0.094; H=0.390, P=0.823); under the blood diet, Wolbachia densities in the ovary and fat body were first decreased and then increased with days of age (H=20.572, P<0.001; H=9.675, P=0.008). Conclusion Feeding blood reduces the density of Wolbachia in the reproductive tissue but increases its density in the non-reproductive tissues of Ae. aegypti mosquitoes of the WB strain. Under laboratory conditions, the mosquitoes can be fed with white sugar to increase the density of Wolbachia in the body.

Key words: Aedes aegypti, Wolbachia, Diet, Density

摘要： 目的 了解不同饮食对WB株埃及伊蚊雌蚊卵巢、脂肪体和其他剩余组织沃尔巴克氏体（Wolbachia）密度的影响。方法 WB株埃及伊蚊羽化后，分为葡萄糖组、白糖组和血餐组。葡萄糖组和白糖组分别喂食葡萄糖和白糖，血餐组在第3天时喂食血餐2 h，分别收集各处理组4、4.5、5和6 d的蚊虫，解剖获取各蚊虫卵巢、脂肪体和其他剩余组织，分别提取DNA，实时荧光定量PCR法检测各组织沃尔巴克氏体密度。采用SPSS 20.0软件作数据分析，Kruskal-Wallis H检验分析不同饮食条件和不同日龄时蚊虫沃尔巴克氏体密度变化的差异。结果 4.5 d时，在葡萄糖、白糖和血餐条件下，埃及伊蚊雌蚊卵巢沃尔巴克氏体密度分别为2.149、2.773和0.761，血餐组与其他2组密度差异有统计学意义（H=40.754，P<0.001）；而蚊虫脂肪体和其他剩余组织沃尔巴克氏体密度在葡萄糖、白糖和血餐条件下分别为0.859、1.189、1.298和0.505、0.405、1.012，均以血餐组最高（H=1.631，P=0.442；H=6.306，P=0.043）。同一日龄3种饮食条件下，蚊虫卵巢沃尔巴克氏体密度在4、5、6 d时血餐组较低，且在4和6 d时白糖组最高（H=14.335，P=0.001；H=22.049，P<0.001；H=4.963，P=0.084）。蚊虫脂肪体4和6 d时的沃尔巴克氏体密度以及其他剩余组织4、5、6 d的沃尔巴克氏体密度，均为血餐组>白糖组>葡萄糖组（H=7.186，P=0.028；H=10.504，P=0.005；H=16.338，P<0.001；H=14.083，P=0.001；H=4.266，P=0.118）。不同日龄同一饮食条件下，葡萄糖组蚊虫卵巢、脂肪体和其他剩余组织沃尔巴克氏体密度随日龄变化差异无统计学意义（H=4.683，P=0.096；H=2.451，P=0.294；H=0.293，P=0.864）；白糖饮食条件下，蚊虫卵巢和脂肪体沃尔巴克氏体密度随日龄增加有增加趋势，但差异无统计学意义（H=4.731，P=0.094；H=0.390，P=0.823）；血餐条件下，蚊虫卵巢和脂肪体沃尔巴克氏体密度均随日龄先降低后上升（H=20.572，P<0.001；H=9.675，P=0.008）。结论 血餐降低WB株埃及伊蚊生殖组织沃尔巴克氏体密度，可增加非生殖组织内沃尔巴克氏体密度。在实验室条件下，可用白糖饲养蚊虫以增加沃尔巴克氏体密度。

关键词: 埃及伊蚊, 沃尔巴克氏体, 饮食, 密度