Resistance testing of Musca domestica to five insecticides in Baiyin city of Gansu province, China

doi:10.11853/j.issn.1003.8280.2022.02.013

Abstract

Abstract: Objective To investigate the resistance of Musca domestica to commonly used insecticides in Baiyin city, Gansu province, China, and to provide evidence for rational use of insecticides, and scientific and sustainable vector control. Methods The WHO micro-drop method was used to determine the resistance to commonly used insecticides of female M. domestica 3-5 days after emergence. Results M. domestica in both Baiyin district and Pingchuan district of Baiyin developed extremely high resistance to DDVP (202.33 and 268.33 times, respectively) and high resistance to pyrethroid insecticides. The median lethal dose (LD₅₀) values of beta-cypermethrin, permethrin, and deltamethrin for M. domestica were 0.621, 0.296, and 0.187 μg/fly in Baiyin district, respectively, and 0.827, 0.497, 0.198 μg/fly in Pingchuan district, respectively. The LD₅₀ of propoxur in Baiyin district and Pingchuan district were 1.467 and 2.183 μg/fly, respectively, which belong to a low resistance level. The resistance levels to the five insecticides were significantly higher in Baiyin district and Pingchuan district (succeeded in creating the national healthy cities) than in Jingyuan county (not applying for the national healthy city; all P<0.05). Conclusion M. domestica in Baiyin city has developed resistance to organophosphate and pyrethroid insecticides. It is recommend to conduct long-term and continuous vector resistance surveillance to guide scientific and rational use of insecticides, and adopt comprehensive sustainable control strategies focusing on environmental governance and physical control.

Key words: Musca domestica, Insecticide resistance, Analysis

摘要： 目的了解甘肃省白银市家蝇对常用杀虫剂的抗药性现状，为合理使用杀虫剂，科学、可持续控制病媒生物提供依据。方法采用世界卫生组织微量点滴法，测定羽化后3~5 d的雌性家蝇对常用杀虫剂的抗性水平。结果白银市白银区和平川区家蝇对敌敌畏已产生极高抗性，分别为202.33和268.33倍；对拟除虫菊酯类杀虫剂均为高抗水平，白银区家蝇对高效氯氰菊酯、氯菊酯和溴氰菊酯的LD₅₀分别为0.621、0.296、0.187 μg/只，平川区家蝇对高效氯氰菊酯、氯菊酯和溴氰菊酯的LD₅₀分别为0.827、0.497、0.198 μg/只；白银区和平川区对残杀威的LD₅₀分别为1.467和2.183 μg/只，属于低抗水平。创卫成功的白银区、平川区家蝇对5种杀虫剂的抗性水平均高于靖远县（非创卫）的水平（均P<0.05）。结论白银市家蝇对有机磷类和拟除虫菊酯类的多种杀虫剂产生抗性，且卫生创建区抗性水平更高，建议长期、连续开展病媒生物抗药性监测，科学合理使用杀虫剂，采取以环境治理和物理防治为主的可持续综合控制策略，控制苍蝇孳生和危害。

关键词: 家蝇, 抗药性, 分析

CLC Number:

R384.2

WANG You-jun, ZHANG Xue-tai, WU Jia-hui. Resistance testing of Musca domestica to five insecticides in Baiyin city of Gansu province, China[J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 234-238.

王友军, 张学太, 吴嘉徽. 甘肃省白银市家蝇对5种杀虫剂的抗药性检测[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 234-238.

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