A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides

doi:10.11853/j.issn.1003.8280.2022.02.002

Chinese Journal of Vector Biology and Control ›› 2022, Vol. 33 ›› Issue (2): 171-176.DOI: 10.11853/j.issn.1003.8280.2022.02.002

• Experimental Study • Previous Articles Next Articles

A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides

LI Wen-yu¹, ZHOU Xin-xin¹, LUN Xin-chang¹, MA De-long¹, LIN Ling-hong^1,2, SONG Xiu-ping¹, LU Liang¹, LIU Qi-yong¹, WANG Jun¹, WANG Chun-lan¹, MENG Feng-xia¹

1. State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Fuzhou Center for Disease Control and Prevention, Fuzhou, Fujian 350004, China

Received:2021-11-22 Online:2022-04-20 Published:2022-05-09
Supported by:
National Science and Technology Major Project of China (No. 2018ZX10101002-002); Emergency Response Mechanism Operation Program, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (No. 131031102000180007)

淡色库蚊成蚊对拟除虫菊酯类杀虫剂敏感性的影响因素研究

李文玉¹, 周欣欣¹, 伦辛畅¹, 马德龙¹, 林凌鸿^1,2, 宋秀平¹, 鲁亮¹, 刘起勇¹, 王君¹, 王春兰¹, 孟凤霞¹

1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, WHO媒介生物监测与管理合作中心, 北京 102206;
2. 福州市疾病预防控制中心, 福建福州 350004

通讯作者: 孟凤霞,E-mail:mengfengxia@icdc.cn
作者简介:李文玉,女,在读硕士,主要从事媒介生物抗药性研究,E-mail:lwy2071@163.com
基金资助:
国家科技重大专项（2018ZX10101002-002）；中国疾病预防控制中心传染病预防控制所公共卫生突发应急反应机制运行项目（131031102000180007）

Abstract

Abstract: Objective To study the factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides, and to provide evidence for the insecticide resistance surveillance and the control of adult Cx. pipiens pallens. Methods The WHO tube test was performed on laboratory Cx. pipiens pallens strains (the Beijing[BJ] strain, Beijing sensitive[BJS] strain, and Tianjin[TJ] strain) to evaluate the insecticide susceptibility of adult test mosquitoes to permethrin and deltamethrin under different conditions. Results In female adult mosquitoes, the sensitivity to permethrin was the BJ strain ≈ the BJS strain > the TJ strain (≈ meaning no significant difference in sensitivity between the two strains), and the median lethal concentrations (LC₅₀) were 0.258% (0.222%, 0.304%), 0.283% (0.239%, 0.334%), and 0.457% (0.413%, 0.508%), respectively. In male adult mosquitoes, the sensitivity to permethrin was the BJ strain > the BJS strain ≈ the TJ strain, and the LC₅₀ values were 0.174% (0.157%, 0.195%), 0.249% (0.213%, 0.290%), and 0.251% (0.205%, 0.294%), respectively. The sensitivity of female adult mosquitoes to deltamethrin was the BJ strain > the BJS strain ≈ the TJ strain, and the LC₅₀ values were 0.024% (0.021%, 0.028%), 0.080% (0.066%, 0.099%), and 0.098% (0.079%, 0.127%), respectively. The sensitivity of male adult mosquitoes to deltamethrin was the BJ strain ≈ the BJS strain, and the LC₅₀ values were 0.038% (0.032%, 0.449%) and 0.018% (0.011%, 0.034%), respectively. The knockdown resistance mutation rate was 15.96% in the BJ strain, while no mutation of the gene was detected in the BJS strain and TJ strain. Under laboratory conditions, the natural mortality of male adult Cx. pipiens pallens was higher than that of female mosquitoes at any age. Under the same conditions, male adult mosquitoes were more sensitive to the insecticides than female adult mosquitoes. The toxicity of deltamethrin was 3.5-10.8 times that of permethrin. Conclusion Age and sex can affect the insecticide susceptibility of adult mosquito populations. Therefore, when carrying out insecticide resistance monitoring or efficacy evaluation, test mosquitoes should be homogeneous.

Key words: Culex pipiens pallens, Adult mosquitoe, Laboratory strain, Pyrethroid insecticide, Resistance, Influencing factor

摘要： 目的研究淡色库蚊成蚊对拟除虫菊酯类杀虫剂敏感性的影响因素，为淡色库蚊成蚊抗性监测及防控提供依据。方法以实验室淡色库蚊品系为研究对象，使用世界卫生组织推荐的成蚊抗性监测接触法，评价在不同条件下不同试虫的杀虫剂敏感性。结果 3个实验室品系中，对氯菊酯的敏感性：雌成蚊北京品系（BJ）≈北京敏感品系（BJS）>天津品系（TJ）（≈表示2个品系对杀虫剂的敏感性差异无统计学意义），半数致死浓度（LC₅₀）分别为0.258%（0.222%~0.304%）、0.283%（0.239%~0.334%）和0.457%（0.413%~0.508%），雄成蚊为BJ>BJS≈TJ，LC₅₀分别为0.174%（0.157%~0.195%）、0.249%（0.213%~0.290%）和0.251%（0.205%~0.294%）。对溴氰菊酯的敏感性：雌成蚊为BJ>BJS≈TJ，LC₅₀分别为0.024%（0.021%~0.028%）、0.080%（0.066%~0.099%）和0.098%（0.079%~0.127%），雄成蚊BJ≈BJS，LC₅₀分别为0.038%（0.032%~0.449%）和0.018%（0.011%~0.034%）。BJ击倒抗性基因（kdr）突变频率为15.96%，BJS和TJ未检测到突变。在实验室控制条件下，不同日龄淡色库蚊雄成蚊的自然死亡率高于雌蚊；在相同条件下，对杀虫剂雄成蚊比雌成蚊敏感；溴氰菊酯对淡色库蚊成蚊的毒力强于氯菊酯3.5~10.8倍。结论成蚊种群日龄和性别的差异均会对杀虫剂敏感性产生影响，故在开展杀虫剂抗药性监测或药效评价时，应注意试虫种群的标准化。

关键词: 淡色库蚊, 成蚊, 实验室品系, 拟除虫菊酯类杀虫剂, 抗药性, 影响因素

CLC Number:

LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MA De-long, LIN Ling-hong, SONG Xiu-ping, LU Liang, LIU Qi-yong, WANG Jun, WANG Chun-lan, MENG Feng-xia. A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides[J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 171-176.

李文玉, 周欣欣, 伦辛畅, 马德龙, 林凌鸿, 宋秀平, 鲁亮, 刘起勇, 王君, 王春兰, 孟凤霞. 淡色库蚊成蚊对拟除虫菊酯类杀虫剂敏感性的影响因素研究[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 171-176.

References

[1] 杨培培. 蚊媒拟除虫菊酯类杀虫剂抗性检测及乙脑病毒携带情况研究[D]. 济南:济南大学, 2016. Yang PP. Study on the detection of pyrethroid insecticide resistance of the vector mosquito and JE virus infection of mosquito[D]. Ji'nan:University of Jinan, 2016. (in Chinese)
[2] 孟凤霞, 靳建超, 陈云, 等. 我国淡色库蚊/致倦库蚊对常用化学杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志, 2011, 22(6):517-520, 528. Meng FX, Jin JC, Chen Y, et al. Resistance of Culex pipiens pallens/Cx. pipiens quinquefasciatus to commonly used insecticides in China[J]. Chin J Vector Biol Control, 2011, 22(6):517-520, 528. (in Chinese)
[3] 宋晓, 程鹏, 王海防, 等. 鲁西南地区淡色库蚊抗药性评价[J]. 中国血吸虫病防治杂志, 2020, 32(1):69-72. DOI:10.16250/j.32.1374.2018261. Song X, Cheng P, Wang HF, et al. Study on insecticide resistance of Culex pipiens pallens in southwest region of Shandong province[J]. Chin J Schisto Control, 2020, 32(1):69-72. DOI:10.16250/j.32.1374.2018261.(in Chinese)
[4] 李菊林, 唐建霞, 吴竞瑶. 等. 2018-2019年江苏省库蚊监测结果分析[J]. 中国血吸虫病防治杂志, 2021, 33(5):510-512, 522. DOI:10.16250/j.32.1374.2020308. Li JL, Tang JX, Wu JY, et al. Surveillance of Culex mosquitoes in Jiangsu province from 2018 to 2019[J]. Chin J Schisto Control, 2021, 33(5):510-512, 522. DOI:10.16250/j.32.1374.2020308.(in Chinese)
[5] 高丹, 吴旭, 周博. 沈阳市淡色库蚊幼虫对5种常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2021, 27(4):384-385. DOI:10.19821/j.1671-2781.2021.04.025. Gao D, Wu X, Zhou B. Investigation on the resistance of Culex pipiens pallens larvae to five commonly used insecticides in Shenyang[J]. Chin J Hyg Insect Equip, 2021, 27(4):384-385. DOI:10.19821/j.1671-2781.2021.04.025.(in Chinese)
[6] 刘维, 赵岩, 郭建华, 等. 2020年吉林省淡色库蚊幼虫对常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2021, 27(6):496-498. DOI:10.19821/j.1671-2781.2021.06.004. Liu W, Zhao Y, Guo JH, et al. Survey on the resistance of Culex pipiens pallens larvae to commonly used insecticides in Jilin province in 2020[J]. Chin J Hyg Insect Equip, 2021, 27(6):496-498. DOI:10.19821/j.1671-2781.2021.06.004.(in Chinese)
[7] 姜志宽, 张桂林. 拟除虫菊酯在卫生害虫防治中的应用[J]. 中华卫生杀虫药械, 2007, 13(5):307-311. DOI:10.3969/j.issn.1671-2781.2007.05.001. Jiang ZK, Zhang GL. Application of pyrethrin for controlling insects[J]. Chin J Hyg Insect Equip, 2007, 13(5):307-311. DOI:10.3969/j.issn.1671-2781.2007.05.001.(in Chinese)
[8] World Health Organization (WHO). Test procedures for insecticide resistance monitoring in malaria vector mosquitoes[M]. 2nd ed. Geneva:WHO, 2018:17-20.
[9] 刘维德. 蚊类抗药性及其测定[M]. 北京:科学出版社, 1979:53-63. Liu WD. Insecticide resistance of mosquitoes and its determination[M]. Beijing:Science Press, 1979:53-63. (in Chinese)
[10] 宋锋林. 淡色库蚊对拟除虫菊酯击倒抗性(kdr)的分子生态学特征的研究[D]. 北京:中国人民解放军军事医学科学院, 2004. Song FL. The study on molecular ecology characteristics of knockdown resistance (kdr) to pyrethroid of Culex pipiens pallens[D]. Beijing:Academy of Military Medical Sciences, 2004. (in Chinese)
[11] 朱昌亮, 李玉兰, 孙立新, 等. 淡色库蚊对溴氰菊酯抗性的遗传分析[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(1):21-24. Zhu CL, Li YL, Sun LX, et al. Genetic analysis of deltamethrin resistance in Culex pipiens pallens[J]. Chin J Parasitol Parasit Dis, 1998, 16(1):21-24. (in Chinese)
[12] 李菊林, 高琪, 周华云, 等. 嗜人按蚊对溴氰菊酯抗性的遗传分析[J]. 中国血吸虫病防治杂志, 2003, 15(5):367-369. DOI:10.3969/j.issn.1005-6661.2003.05.014. Li JL, Gao Q, Zhou HY, et al. Genetic analysis of deltamethrin resistance in Anopheles anthropophagus[J]. Chin J Schisto Control, 2003, 15(5):367-369. DOI:10.3969/j.issn.1005-6661.2003.05.014.(in Chinese)
[13] 张小磊, 袁志明, 胡晓敏. 致倦库蚊对杀虫剂的抗性现状及治理[J]. 中国媒介生物学及控制杂志, 2019, 30(6):719-724. DOI:10.11853/j.issn.1003.8280.2019.06.031. Zhang XL, Yuan ZM, Hu XM. Current status of insecticide resistance in Culex pipiens quinquefasciatus and management of resistance[J]. Chin J Vector Biol Control, 2019, 30(6):719-724. DOI:10.11853/j.issn.1003.8280.2019.06.031.(in Chinese)
[14] Farnham AW. Genetics of resistance of houseflies (Musca domestica L.) to pyrethroids. I. Knock-down resistance[J]. Pestic Sci, 1977, 8(6):631-636. DOI:10.1002/ps.2780080607.
[15] 张晓越. 我国淡色库蚊/致倦库蚊对常用杀虫剂抗性时空分布的研究[D]. 北京:中国疾病预防控制中心, 2014. Zhang XY. Study on space-time distribution of Culex pipens pallens/quinquefasciatus resistance to common-used insecticides in China[D]. Beijing:Chinese Center for Disease Control and Prevention, 2014. (in Chinese)
[16] Liu H, Lu YJ, Liu QY, et al. Comparison of pyrethroid resistance in adults and larvae of Culex pipiens pallens (Diptera:Culicidae) from four field populations in China[J]. J Econ Entomol, 2013, 106(1):360-365. DOI:10.1603/EC11439.

A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides

淡色库蚊成蚊对拟除虫菊酯类杀虫剂敏感性的影响因素研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Si-han, ZHOU Xin-xin, KE Xue-mei, LI Yu-wei, CHEN Bin, HUANG Jing-wen, CHEN Hua-fang, LIN Min-zhen, LEI Lei, GUO Zhi-nan, MENG Feng-xia. Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 177-182.
[2]	WANG You-jun, ZHANG Xue-tai, WU Jia-hui. Resistance testing of Musca domestica to five insecticides in Baiyin city of Gansu province, China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 234-238.
[3]	ZHAO Chun-chun, ZHOU Xin-xin, LI Wen-yu, LUN Xin-chang, LIU Xiao-bo, WU Hai-xia, WANG Jun, LIU Qi-yong, MENG Feng-xia. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 30-37.
[4]	ZHENG Yu-ting, YANG Chun-mei, YANG Ming-dong, JIANG Jin-yong. Ecological and insecticide resistance surveillance of dengue vector Aedes in Yunnan border region of China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 38-43.
[5]	TU Tao-tian, XIAO Han-sen, MENG Feng-xia, LIU Qi-yong, LIU Xiao-bo, HE Ya-ming, JI Heng-qing. An investigation of the insecticide resistance of Aedes vector after emergency control of a dengue fever outbreak in Chongqing， China， 2019 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 44-47.
[6]	WU Zhi-ming, CHU Hong-liang. An investigation of mosquito overwintering in urban environment of Jiangsu province， China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 83-88.
[7]	LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 104-107.
[8]	ZHOU Yi-bin, YAO Jun-yi, ZHU Yi-yi, ZHU Jiang, LENG Pei-en, WU Huan-yu. A study of time-frequency model for monitoring Culex pipiens pallens by CO₂ mosquito lamps [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 137-142.
[9]	ZHOU Chang-yin, GUAN Qing-qing, DAI Yu-qi, LIU Hong-xia, QIAN Kun. Expression characterization and functional analysis of juvenile hormone receptor gene CpMet in Culex pipiens pallens [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 680-685.
[10]	XU Xue-lian, HAN A-xiang, YE Shi-qing, GUAN Wan-chun, LOU Yong-liang. Metagenomic analysis of microbial community structure, antibiotic resistance genes, and virulence factors of ticks captured at Wenzhou port, Zhejiang province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 763-771.
[11]	WU Li, YIN Jia-xiang. An overview of the relationship between fleas and environmental factors [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 779-782.
[12]	ZHAO Qiong-yao, QIU Xing-hui, YANG Yan, LUO Xing, SONG Xiao-ming, JIA Yong-chao. Polymorphism and resistance mutation frequency of the target genes of insecticides in Anopheles sinensis in Guangyuan of Sichuan province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(5): 541-545.
[13]	HU Yuan-feng, TAN Liang-fei. Surveillance results of cockroach density and insecticide resistance in Ezhou of Hubei province, China, 2016-2020 [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 599-603.
[14]	WANG Yang, LIU Hong-mei, GUO Xiu-xia, SONG Xiao, WANG Hai-yang, CHENG Peng, WANG Hai-fang, WANG Huai-wei, GONG Mao-qing. Molecular cloning and expression analysis of cuticular protein gene CpCPR117 in Culex pipiens pallens [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 436-440.
[15]	CHEN Xiao-min, LIU Qin, ZHOU Liang-cai, WU Li-qun, BAO Ji-yong, WU Tai-ping. An investigation of resistance of Musca domestica to commonly used insecticides in Wuhan, China, 2009-2015 [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 468-471.