Abstract: Objective To identify the adult flies and pupae captured in the entry goods by Qingdao Airport Customs using morphological and DNA barcoding techniques. Methods Morphological identification was performed for the flies captured, and one leg of the fly and the pupae were used to extract genomic DNA. The DNA barcoding technique was used for the amplification and sequencing of the genomic DNA of this unknown fly species, and the sequences obtained were compared with those in GenBank database of National Center for Biotechnology Information and Barcode of Life Data Systems (BOLD) to construct an evolutionary tree. Results Morphological identification showed that the flies were identified as Phoridae in Diptera. DNA barcoding identification and sequence alignment showed that the DNA barcoding sequences of the adult fly and pupae were >99.00% homologous with the sequence of Megaselia spiracularis in BOLD and GenBank, and with reference to morphological identification, the species was identified as M. spiracularis. Conclusion The adult fly and pupae captured are identified as M. spiracularis, which is found in the entry goods by Qingdao Airport Customs for the first time.

Key words: DNA barcoding, Megaselia spiracularis, Qingdao

摘要： 目的 对青岛机场海关截获的1批入境成蝇和蝇蛹进行形态和DNA条形码鉴定。方法 对截获的蝇类，先进行形态学鉴定，然后取蝇的1条足及1只蛹分别提取基因组DNA，利用DNA条形码技术，对该未知蝇类基因组DNA进行扩增和序列分析，与美国国立生物技术信息中心（NCBI）GeneBank数据库及生命条形码系统（BOLD）中的序列进行比对，构建进化树。结果 形态学鉴定为双翅目蚤蝇类，通过DNA条形码鉴定和序列比对，成蝇及蛹的DNA条形码序列与GenBank和BOLD中东亚异蚤蝇的序列同源性>99.00%，结合形态学鉴定结果，判断该蝇种成虫及蛹为东亚异蚤蝇。结论 所捕获的蝇类成虫与蛹为东亚异蚤蝇，为青岛机场海关首次在入境货物中发现。

关键词: DNA条形码, 东亚异蚤蝇, 青岛