Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021

doi:10.11853/j.issn.1003.8280.2022.01.019

Abstract

Abstract: Objective To investigate the resistance of larval and adult Culex pipiens pallens to commonly used insecticides in the Dongping Lake area of Shandong province, China, and to provide evidence for rational use of insecticides for mosquito control. Methods The field populations of Cx. pipiens pallens were collected from the Dongping Lake area on July 7, 2021, and were reared to F1 generation in the laboratory. According to the bioassay methods of World Health Organization, the impregnation method was used for larval tests, and the median lethal dose and regression equation were calculated; the cylinder bioassay was used to determine the resistance of adult mosquitoes. The results were recorded with Excel 2019 software for statistical analysis. Results The resistance ratio of Cx. pipiens pallens larvae in the Dongping Lake area was 8.55 to DDVP (low resistance), 6.52 to propoxur (low resistance), 180.00 to deltamethrin (high resistance), 100.50 to cypermethrin (high resistance), and 1.50 to Bacillus thuringiensis subsp. israelensis (susceptible). For adult Cx. pipiens pallens, the mortality rate within 24 h after 1-h insecticide exposure was 16.00% for 0.025% deltamethrin (high resistance), 20.00% for 0.025% beta-cypermethrin (high resistance), 100% for 5% malathion (susceptible), and 88.00% for 0.01% propoxur (possible resistance). Conclusion Cx. pipiens pallens mosquitoes in the Dongping Lake area of Shandong province have developed varying degrees of resistance to the commonly used insecticides. It is necessary to strengthen the monitoring of mosquito resistance to insecticides in this region and take rational mosquito control measures to mitigate the development of mosquito resistance to insecticides and improve the effect of prevention and control.

Key words: Culex pipiens pallens, Insecticide resistance, Control strategy

摘要： 目的检测山东省东平湖地区淡色库蚊幼虫与成蚊对常用杀虫剂的抗性情况，为合理使用杀虫剂防控蚊虫提供依据。方法 2021年7月7日从东平湖地区采集淡色库蚊野外幼虫带回实验室饲养至F1代，按照世界卫生组织生物测定法，幼虫测试采用浸渍法，计算半数致死浓度和回归方程；成蚊测试采用接触筒法测定抗性水平，并将结果录入Excel 2019软件进行统计分析。结果 2021年，东平湖地区淡色库蚊幼虫对敌敌畏、残杀威、溴氰菊酯、氯氰菊酯、苏云金杆菌以色列亚种（Bti）的抗性倍数分别为8.55、6.52、180.00、100.50和1.50倍，对敌敌畏和残杀威表现为低度抗性，对溴氰菊酯和氯氰菊酯表现为高度抗性，对Bti表现为敏感；将淡色库蚊成蚊暴露于0.025%溴氰菊酯、0.025%高效氯氰菊酯、5%马拉硫磷和0.01%残杀威各1 h，24 h死亡率分别为16.00%、20.00%、100%和88.00%，成蚊对溴氰菊酯和高效氯氰菊酯呈高度抗性，对马拉硫磷和残杀威呈敏感和可疑抗性。结论山东省东平湖地区淡色库蚊对常用杀虫剂均产生了不同程度的抗药性，应当加强该地区蚊虫抗药性监测，采取合理的治理手段，延缓蚊虫抗药性发展，提高防控效果。

关键词: 淡色库蚊, 抗药性, 防治方法

CLC Number:

LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021[J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 104-107.

吕文祥, 程鹏, 彭荟, 王海洋, 王海防, 郭秀霞, 张崇星, 刘宏美, 公茂庆, 刘丽娟. 山东省东平湖地区2021年淡色库蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 104-107.

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