A study of wild midges carrying Tibet orbivirus in Hainan province, China

doi:10.11853/j.issn.1003.8280.2021.04.006

Chines Journal of Vector Biology and Control ›› 2021, Vol. 32 ›› Issue (4): 415-421.DOI: 10.11853/j.issn.1003.8280.2021.04.006

Special Issue: 媒介生物病原学监测专题

• Vector Etiological Surveillance Special Topic • Previous Articles Next Articles

A study of wild midges carrying Tibet orbivirus in Hainan province, China

WANG Qin-yan^1,2, FU Shi-hong², SUN Ding-wei³, XU Xiu-yan^1,2, WU Qun³, ZENG Lin-hai³, LI Shan-gan³, HE Ying², LI Fan², NIE Kai², XU Song-tao², YIN Qi-kai², WANG Huan-yu², LU Xiao-qing¹, LIANG Guo-dong²

1. Public Health Institute, Qingdao University, Qingdao, Shandong 266071, China;
2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
3. Hainan Center for Disease Control and Prevention, Haikou, Hainan 570203, China

Received:2021-03-05 Online:2021-08-20 Published:2021-08-20
Supported by:
Supported by the National Natural Science Foundation of China (No.81290342), Project of State Key Laboratory of Infectious Disease Prevention and Control (No. 2014SKLID103) and the Key R&D Program of Hainan Province (No. ZDYF2017111)

海南省野生蠓虫西藏环状病毒的研究

王琴燕^1,2, 付士红², 孙定炜³, 许秀燕^1,2, 吴群³, 曾林海³, 李善干³, 何英², 李樊², 聂凯², 许松涛², 殷启凯², 王环宇², 鲁晓晴¹, 梁国栋²

1. 青岛大学公共卫生学院, 山东青岛 266071;
2. 中国疾病预防控制中心病毒病预防控制所, 传染病预防控制国家重点实验室, 北京 102206;
3. 海南省疾病预防控制中心, 海南海口 570203

通讯作者: 鲁晓晴,E-mail:Luxq532@126.com;梁国栋,E-mail:gdliang@hotmail.com
作者简介:王琴燕,女,在读硕士,主要从事虫媒病毒分离鉴定工作,E-mail:2738781242@qq.com;付士红,女,主管技师,主要从事病毒性病原生物相关研究,E-mail:shihongfu@hotmail.com;孙定炜,男,硕士,助理研究员,主要从事寄生虫病防治研究,E-mail:sdw_bmjc@163.com
基金资助:
国家自然科学基金（81290342）；传染病预防控制国家重点实验室项目（2014SKLID103）；海南省重点研发计划（ZDYF2017111）

Abstract

Abstract: Objective To investigate the species and prevalence of blood-sucking insects and their viruses in Qiongzhong county, Hainan province, China. Methods In August 2019, UV mosquito light-trapas (Gongfu Xiaoshuai) were used to collect specimens in the local environment (sheep pens and pig pens) where blood-sucking insects bred, and dry ice was used to transport the specimens to the laboratory. Virological and molecular biological methods were used to isolate viruses and identify the virus isolates. Results A total of 34 mosquitoes from 3 genera (including 26 from Culex, 1 from Anopheles, and 7 Armigeres subalbatus mosquitoes) and 1 450 midges (to be identified) were collected. A virus isolate (HNQZ1927) was isolated from the collected midge specimens, which could cause cytopathic effect in mosquito cells (C6/36), but did not cause cytopathic effect in BHK-21 cells. The gene amplification and nucleotide sequencing and analysis showed that HNQZ1927 was a 10-gene segment orbivirus. The nucleotides (amino acids) in the coding region of HNQZ1927 had 79.4%-98.7% (94.6%-100%) homology with those of Tibet orbivirus (TIBOV) from the genus Orbivirus, and had 1.2%-78.1% homology with those of other orbiviruses except TIBOV. The phylogenetic analysis of the viral genome showed that the HNQZ1927 virus was in the same evolutionary branch as TIBOV XZ0906, which was previously isolated from An. maculatus collected in Tibet, and the TIBOV isolated from Cx. tritaeniorhynchus, Cx. fatigans, and some species of midges in China. Conclusion The HNQZ1927 virus isolated from midges in Hainan province is TIBOV, which is the first time that TIBOV has been isolated from midge specimens collected from nature in Hainan province, China.

Key words: Orbivirus, Tibet orbivirus, Midge, Hainan province

摘要： 目的调查海南省琼中县吸血昆虫及其携带病毒的种类以及流行情况。方法 2019年8月在当地吸血昆虫孳生环境（羊圈、猪圈）中，使用“功夫小帅”牌紫外诱蚊灯进行标本采集并使用干冰运输到实验室。采用病毒学和分子生物学方法分离病毒并对病毒分离物进行鉴定。结果共采集到蚊虫3属34只（其中库蚊26只、按蚊1只和骚扰阿蚊7只）和蠓虫（待鉴定）1 450只。从采集的蠓虫标本中分离到1株可以引起白纹伊蚊C6/36细胞病变的病毒分离株（HNQZ1927），该病毒不引起金黄地鼠肾细胞（BHK-21）病变。经病毒基因扩增和核苷酸序列测定与分析，结果显示HNQZ1927为10个基因节段的环状病毒属病毒，该病毒基因编码区核苷酸（氨基酸）与环状病毒属中的西藏环状病毒（TIBOV）同源性为79.4%～98.7%（94.6%～100%），而与TIBOV以外的其他环状病毒同源性在1.2%～78.1%。病毒基因组系统进化分析表明，HNQZ1927病毒与我国此前在西藏地区采集的圆斑按蚊中分离的TIBOV XZ0906及我国在三带喙库蚊、致倦库蚊和多种蠓虫中分离的TIBOV处于共同进化分支。结论海南省蠓虫分离的HNQZ1927病毒为TIBOV，这是首次从海南省自然界采集的蠓虫标本中分离到TIBOV 。

关键词: 环状病毒, 西藏环状病毒, 蠓虫, 海南省

CLC Number:

WANG Qin-yan, FU Shi-hong, SUN Ding-wei, XU Xiu-yan, WU Qun, ZENG Lin-hai, LI Shan-gan, HE Ying, LI Fan, NIE Kai, XU Song-tao, YIN Qi-kai, WANG Huan-yu, LU Xiao-qing, LIANG Guo-dong. A study of wild midges carrying Tibet orbivirus in Hainan province, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 415-421.

王琴燕, 付士红, 孙定炜, 许秀燕, 吴群, 曾林海, 李善干, 何英, 李樊, 聂凯, 许松涛, 殷启凯, 王环宇, 鲁晓晴, 梁国栋. 海南省野生蠓虫西藏环状病毒的研究[J]. 中国媒介生物学及控制杂志, 2021, 32(4): 415-421.

References

[1] Belaganahalli MN, Maan S, Maan NS, et al. Genetic characterization of the tick-borne orbiviruses[J]. Viruses, 2015, 7(5):2185-2209. DOI:10.3390/v7052185.
[2] Attoui H, Becnel J, Belaganahalli S, et al. Part II:The Viruses-the double stranded RNA viruses-family Reoviridae[R]. San Diego:Elsevier Academic Press, 2012.
[3] Kapoor A, Tesh RB, Duraisamy R, et al. A novel mosquito-borne Orbivirus species found in South-east Asia[J]. J Gen Virol, 2013, 94(5):1051-1057. DOI:10.1099/vir.0.046748-0.
[4] Calvo-Pinilla E, Castillo-Olivares J, Jabbar T, et al. Recombinant vaccines against bluetongue virus[J]. Virus Res, 2014, 182:78-86. DOI:10.1016/j.virusres.2013.11.013.
[5] Zientara S, Lecollinet S, Weyer CT. African horse sickness[J]. Rev Sci Tech, 2015, 34(2):315-327. DOI:10.20506/rst.34.2.2359.
[6] Maclachlan NJ, Zientara S, Savini G, et al. Epizootic haemorrhagic disease[J]. Rev Sci Tech, 2015, 34(2):341-351. DOI:10.20506/rst.34.2.2361.
[7] Li MH, Zheng YY, Zhao GY, et al. Tibet orbivirus, a novel Orbivirus species isolated from Anopheles maculatus mosquitoes in Tibet, China[J]. PLoS One, 2014, 9(2):e88738. DOI:10.1371/journal.pone.0088738.
[8] Wu D, Tan QQ, Zhang H, et al. Genomic and biological features of a novel orbivirus isolated from mosquitoes, in China[J]. Virus Res, 2020, 285:197990. DOI:10.1016/j.virusres.2020.197990.
[9] Lei WW, Guo XF, Fu SH, et al. Isolation of Tibet orbivirus, TIBOV, from Culicoides collected in Yunnan, China[J]. PLoS One, 2015, 10(8):e0136257. DOI:10.1371/journal.pone.0136257.
[10] Wang JL, Li HC, He YW, et al. Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China[J]. Virol J, 2017, 14(1):105. DOI:10.1186/s12985-017-0774-9.
[11] 范娜, 曹玉玺, 付士红, 等. 三带喙库蚊中分离的西藏环状病毒全基因序列测定及分析[J]. 病毒学报, 2018, 34(5):625-637. DOI:10.13242/j.cnki.bingduxuebao.003452.Fan N, Cao YX, Fu SH, et al. Genome sequencing and analyses of the Tibet orbivirus isolated from Culex tritaeniorhynchus[J]. Chin J Virol, 2018, 4(5):625-637. DOI:10.13242/j.cnki.bingduxuebao.003452.
[12] Xing SZ, Guo XF, Zhang XLL, et al. A novel mosquito-borne reassortant orbivirus isolated from Xishuangbanna, China[J]. Virol Sin, 2017, 32(2):159-162. DOI:10.1007/s12250-016-3886-2.
[13] 游志勇, 王逸民, 赵子江, 等. 海南岛两个虫媒病毒分离物的初步鉴定[J]. 病毒学报, 1988, 4(1):11-17.You ZY, Wang YM, Zhao ZJ, et al. Preliminary identification of two arbovirus isolates from Hainan island[J]. Chin J Virol, 1988, 4(1):11-17.
[14] 周俊梅, 江丽芳, 高阳, 等. 登革2型病毒海南分离株全长E基因的测定与分析[J]. 中华微生物学和免疫学杂志, 2003, 23(4):304-306. DOI:10.3760/j:issn:0254-5101.2003.04.021.Zhou JM, Jiang LF, Gao Y, et al. Gene sequencing and analysis of the envelope protein of dengue 2 virus isolated in Hainan China[J]. Chin J Microbiol Immunol, 2003, 23(4):304-306. DOI:10.3760/j:issn:0254-5101.2003.04.021.
[15] 董必军, 陈文州, 李秀维, 等. 首次从海南岛蚊虫和蝙蝠中分离出两株基孔肯雅病毒[J]. 中国媒介生物学及控制杂志, 1993, 4(3):205-208.Dong BJ, Chen WZ, Li XW, et al. Two isolates of Chikungunya virus isolated from Culex fatigans and bats, for the first time in Hainan, China[J]. Chin J Vector Biol Control, 1993, 4(3):205-208.
[16] 游志勇, 王逸民, 赵治国, 等. 海南省两株环状病毒的分离与鉴定[J]. 病毒学报, 1990, 6(3):272-276.You ZY, Wang YM, Zhao ZG, et al. Isolation and identification of two strains of orbivirus in Hainan province[J]. Chin J Virol, 1990, 6(3):272-276.
[17] 郑雅匀, 王志玉, 梁国栋. 海南省虫媒病毒调查研究状况[J]. 中国媒介生物学及控制杂志, 2011, 22(6):607-610.Zheng YY, Wang ZY, Liang GD. Review on arbovirus studies in Hainan province, China[J]. Chin J Vector Biol Control, 2011, 22(6):607-610.
[18] Song S, Li YY, Fu SF, et al. Could Zika virus emerge in mainland China? Virus isolation from nature in Culex quinquefasciatus, 2016[J]. Emerg Microbes Infect, 2017, 6(11):e93. DOI:10.1038/emi.2017.80.
[19] Fu SH, Song S, Liu H, et al. Zika virus isolated from mosquitoes:a field and laboratory investigation in China, 2016[J]. Sci China Life Sci, 2017, 60(12):1364-1371. DOI:10.1007/s11427-017-9196-8.
[20] Feng Y, Li YY, Fu SH, et al. Isolation of Kaeng Khoi virus (KKV) from Eucampsipoda sundaica bat flies in China[J]. Virus Res, 2017, 238:94-100. DOI:10.1016/j.virusres.2017.06.007.
[21] Ren XJ, Fu SH, Dai PF, et al. Pigsties near dwellings as a potential risk factor for the prevalence of Japanese encephalitis virus in adult in Shanxi, China[J]. Infect Dis Poverty, 2017, 6(1):100. DOI:10.1186/s40249-017-0312-4.
[22] Hassan SS, Roy P. Expression and functional characterization of bluetongue virus VP2 protein:role in cell entry[J]. J Virol, 1999, 73(12):9832-9842. DOI:10.1128/JVI.73.12.9832-9842.1999.
[23] Liang GD, Li XL, Gao XY, et al. Arboviruses and their related infections in China:a comprehensive field and laboratory investigation over the last 3 decades[J]. Rev Med Virol, 2018, 28(1):e1959. DOI:10.1002/rmv.1959.

A study of wild midges carrying Tibet orbivirus in Hainan province, China

海南省野生蠓虫西藏环状病毒的研究

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