Molecular and morphological identification of Cheyletus malaccensis and C. carnifex

doi:10.11853/j.issn.1003.8280.2021.02.022

Abstract

Abstract: Objective To investigate methods for the morphological and molecular identification of Cheyletus malaccensis and C. carnifex. Methods From May 2018 to July 2019, C. malaccensis and C. carnifex were obtained from the storage samples of some farmers' granaries in several areas of Anhui province. The morphological characteristics of C. malaccensis and C. carnifex were compared and described. MEGA X software was used to analyze mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene sequence for C. malaccensis and C. carnifex, and the maximum likelihood method was used to construct the phylogenetic tree. Results C. malaccensis and C. carnifex had similar morphological characteristics, while morphological identification could be performed under a light microscope based on the shape of peritremes, the morphology of guard seta of tarsus I, and the length-ratio between solenidia and pretarsus of tarsus I. Based on the COⅠ gene, the interspecific genetic distance was >0.02 and the intraspecific genetic distance was <0.02. The base composition analysis showed that the sequence had an obvious A+T bias, and the results of BLAST were consistent with those of morphological identification. Phylogenetic tree showed that C. malaccensis and C. carnifex were clustered into one single branch, respectively. Conclusion Identification of C. malaccensis and C. carnifex can be performed based on the external morphological characteristics and the mitochondrial COⅠ gene, and the combination of these two methods can improve the accuracy of the identification of closely related species.

Key words: Cheyletus malaccensis, Cheyletus carnifex, Morphological identification, Mitochondrial cytochrome c oxidase subunit Ⅰ gene, Molecular identification

摘要： 目的建立马六甲肉食螨与转开肉食螨的形态学和分子鉴定方法。方法 2018年5月至2019年7月，从安徽省部分地区农户粮仓的储藏物样本中发现并采集马六甲肉食螨和转开肉食螨，比较2种肉食螨的形态特征，使用MEGA X软件对2种肉食螨的线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列进行比对分析，最大似然法构建系统发育树。结果马六甲肉食螨与转开肉食螨形态特征相似，但在光镜下仍可通过气门板形状、足Ⅰ跗节管毛的形态及其与支持毛的相对比例等特征进行形态学鉴定。基于COⅠ基因种间遗传距离>0.02，种内遗传距离均<0.02，碱基组成分析发现此段序列具有明显的A+T偏向性，BLAST比对结果与形态学鉴定一致，系统发育树显示转开肉食螨与马六甲肉食螨均能各自聚为一支。结论马六甲肉食螨和转开肉食螨成螨可以依据外部形态特征进行形态学鉴定，也能基于线粒体COⅠ基因进行分子鉴定。2种鉴定方法相结合，可提高近缘种鉴别的准确性。

关键词: 马六甲肉食螨, 转开肉食螨, 形态学鉴定, 线粒体细胞色素C氧化酶亚基Ⅰ基因, 分子鉴定

CLC Number:

R384.4

LI Xin-mei, SUN Meng-tao, ZHAN Yu-juan, ZHANG Lan-xiang, LI Meng-zhu, TAO Dong-dong, SUN En-tao. Molecular and morphological identification of Cheyletus malaccensis and C. carnifex[J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 230-234.

李心玫, 孙梦涛, 詹雨娟, 张兰香, 李梦珠, 陶东东, 孙恩涛. 马六甲肉食螨与转开肉食螨的形态及分子鉴定[J]. 中国媒介生物学及控制杂志, 2021, 32(2): 230-234.

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