Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China

doi:10.11853/j.issn.1003.8280.2021.02.020

Chines Journal of Vector Biology and Control ›› 2021, Vol. 32 ›› Issue (2): 221-223.DOI: 10.11853/j.issn.1003.8280.2021.02.020

• Taxonomic Identification • Previous Articles Next Articles

Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China

XUE Hong-mei, XIE Hui, MA Li, ZHANG Ai-ping, YU Shou-hong, LI Ji-quan, YANG Xu-xin, XU Li-qing

Department of Brucellosis Prevention and Control, Qinghai Provincial Institute of Endemic Disease Prevention and Control, Xining, Qinghai 810021, China

Received:2020-05-12 Online:2021-04-20 Published:2021-04-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81860588)

1株分离于青海省喜马拉雅旱獭的布鲁氏菌微量生化鉴定

薛红梅, 谢辉, 马丽, 张爱萍, 于守鸿, 李积权, 杨旭欣, 徐立青

青海省地方病预防控制所布鲁氏菌病预防控制科, 青海西宁 810021

通讯作者: 徐立青,E-mail:qhxlq2006@163.com
作者简介:薛红梅,女,硕士,主管医师,主要从事布病防治工作,E-mail:qhxhm2009@126.com
基金资助:
国家自然科学基金（81860588）

Abstract

Abstract: Objective To perform microbiochemical identification of a suspected Brucella strain isolated from Marmota himalayana in Qinghai province, China in 2017. Methods The isolated bacteria strains were detected by microbiochemical tube and routine pathogenic identification, and the results were compared with the results of three standard Brucella strains. Results Microbiochemical identification showed that the strain could oxidize urea and did not oxidize ornithine, lysine, D-galactose, sorbitol, xylose, and gluconate. Therefore, it was identified as Brucella melitensis. Conclusion Microbiochemical identification of bacteria is rapid and simple, and can provide an experimental basis for the detection of Brucella.

Key words: Marmota himalayana, Isolated bacteria strain, Microbiochemical identification, Qinghai province

摘要： 目的对2017年自青海省喜马拉雅旱獭组织中分离出的1株疑似布鲁氏菌菌株进行生化鉴定。方法采用细菌微量生化管和常规病原学鉴定，对分离出的菌株进行检测，同时与3种布鲁氏菌标准菌株的检测结果进行对比。结果微量生化鉴定显示，该菌株可氧化尿素，不氧化鸟氨酸、赖氨酸、D-半乳糖、山梨醇、木糖、葡萄糖酸盐，为羊种布鲁氏菌。结论细菌微量生化鉴定方法快速简单，可为布鲁氏菌检验提供一个快速的辅助实验依据。

关键词: 喜马拉雅旱獭, 分离菌株, 微量生化鉴定, 青海省

CLC Number:

R378.5

XUE Hong-mei, XIE Hui, MA Li, ZHANG Ai-ping, YU Shou-hong, LI Ji-quan, YANG Xu-xin, XU Li-qing. Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 221-223.

薛红梅, 谢辉, 马丽, 张爱萍, 于守鸿, 李积权, 杨旭欣, 徐立青. 1株分离于青海省喜马拉雅旱獭的布鲁氏菌微量生化鉴定[J]. 中国媒介生物学及控制杂志, 2021, 32(2): 221-223.

References

[1] 吴海莲,吴海生,何建,等. 青海省果洛藏族自治州鼠疫耶尔森菌病原学分析及耐药相关基因检测[J]. 中国媒介生物学及控制杂志,2019,30(2):167-171. DOI:10.11853/j.issn.1003. 8280.2019.02.012.Wu HL,Wu HS,He J,et al. Etiological analysis and determination of antibiotic resistance-related genes of Yersinia pestis in Guoluo Tibetan autonomous prefecture,Qinghai province,China[J]. Chin J Vector Biol Control,2019,30(2):167-171. DOI:10.11853/j.issn.1003.8280.2019.02.012.
[2] 崔丽瑾,王兴龙,王英超,等. 野生动物布鲁氏菌病[J].中国人兽共患病学报,2010,26(3):283-288.Cui LJ,Wang XL,Wang YC,et al. Brucellosis of wild animals[J]. Chin J Zoonoses,2010,26(3):283-288.
[3] 朴范泽. 家畜传染病学[M]. 北京:中国科学文化出版社,2004:110.Piao FZ. Livestock epidemiology[M]. Beijing:China Science and Culture Press,2004:110.
[4] Ferroglio E,Rossi L,Gennero S. Lung-tissue extract as an alternative to serum for surveillance for brucellosis in chamois[J]. Prev Vet Med,2000,43(2):117-122. DOI:10.1016/S0167-5877(99)00090-2.
[5] Rhyan JC,Quinn WJ,Stackhouse LS,et al. Abortion caused by Brucella abortus biovar 1 in a free-ranging bison (Bison bison) from Yellowstone National Park[J]. J Wildl Dis,1994,30(3):445-446. DOI:10.7589/0090-3558-30.3.445.
[6] Rhyan JC,Gidlewski T,Roffe TJ,et al. Pathology of brucellosis in bison from Yellowstone National Park[J]. J Wildl Dis,2001,37(1):101-109. DOI:10.7589/0090-3558-37.1.101.
[7] 蒋和柱,李富忠,薛梅,等. 旱獭和家兔布鲁氏菌病血清学调查报告[J],现代预防医学,2001,28(1):128.Jiang HZ,Li FZ,Xue M,et al. Serologic investigation of brucellosis in marmots and rabbits[J]. Mod Prev Med,2001,28(1):128.
[8] 金根源,史丕裕,李全,等. 新疆野生动物布鲁氏菌感染情况调查[J],新疆畜牧业,1991(2):43-44. DOI:10.16795/j.cnki.xjxmy.1991.02.021.Jin GY,Shi PY,Li Q,et al. Investigation of brucellosis infection in wild animals in Xinjiang[J]. Xinjiang Anim Husbandry,1991(2):43-44. DOI:10.16795/j.cnki.xjxmy.1991.02.021.
[9] 赵青,贾鸿滨. 天峻野生旱獭布氏杆菌病血清学调查[J].青海畜牧兽医杂志,1994(5):29.Zhao Q,Jia HB. Serological investigation of brucellosis in wild marmots from Tianjun[J]. Qinghai J Anim Husbandry Vet Med,1994(5):29.
[10] 柳秀兰,王晓润. 野生啮齿动物和鸟类布鲁氏菌病血清学检测[J].畜牧兽医,2015,47(6):162.Liu XL,Wang XR. Serological detection of brucellosis in wild rodents and birds[J]. Anim Husbandry Vet Med,2015,47(6):162.
[11] 姜顺求.布鲁氏菌病防治手册[M].北京:人民卫生出版社,1986:41-77.Jiang SQ. Brucellosis prevention manual[M]. Beijing:People's Medical Publishing House,1986:41-77.
[12] 马翔,马纪平.影响赖氨酸、鸟氨酸、精氨酸脱羧酶试验结果的主要因素探讨及培基有效期观察[J].中华医学检验杂志,1991,14(5):302-304.Ma X,Ma JP. The main factors affecting the decarboxylase test results of lysine,ornithine and arginine were discussed and the expiry date of culture medium was observed[J].Chin J Med Lab Med,1991,14(5):302-304.
[13] 叶应妩,王毓三. 全国临床检验操作规程[M].2版.南京:东南大学出版社,1997:573.Ye YW,Wang YS. National operating rules for clinical examination[M]. 2nd ed. Nanjing:Southeast University Press,1997:573.
[14] 袁宏,马翔. 国产生化微量管对肠杆菌科细菌的应用评价[J].医学新知杂志,2004,14(3):205-206.Yuan H,Ma X. Application evaluation of domestic biochemical microtube to enterobacteriaceae bacteri[J]. J Med Sci,2004,14(3):205-206.

Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China

1株分离于青海省喜马拉雅旱獭的布鲁氏菌微量生化鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	ZHANG Ai-ping, XU Li-qing, MA Li, LI Ji-quan, XUE Hong-mei, WANG Jian-ling, YANG Xu-xin, XIE Hui, ZHAO Yuan-bo, YU Shou-hong. Identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 217-220.
[2]	WANG Rong, LIU Qi-yong, LIU Gui-xiang, LI Shou-jiang, MA Yong-cheng, SHI Yan, MA Bin-zhong, MA Zhong-wen, JIANG Ming-xia, GUO Yu-hong. First discovery of Culex (Culex) vagans Wiedemann in Ledu county, Qinghai province, China [J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 209-211.
[3]	TANG Xin-yuan, WANG Mei, CHEN Hong-jian, LI Hong-ying, CAI Jia-zuo, HE Duo-long, CUI Bai-zhong. Epidemiological characteristics of plague in Qinghai province based on geographic information system [J]. Chines Journal of Vector Biology and Control, 2018, 29(6): 604-608.
[4]	LI Hai-long, MA Ying, WEI You-wen, LI Chao, ZHENG Yi, WANG Xue, YANG Ning. Investigation of geographical distribution pattern of rodents in Qinghai province, China [J]. Chines Journal of Vector Biology and Control, 2013, 24(5): 418-421.
[5]	WEI You-wen, MA Ying, LI Hai-long, ZHENG Yi, LI Chao, WANG Xue, LI Xiang, YANG Ning, LUO Jun. Study on spatial niches of rodents in Geermu area of Qinghai province, China [J]. Chines Journal of Vector Biology and Control, 2013, 24(4): 292-294.
[6]	ZHENG Yi, MA Ying, LUO Jun, ZHAO Xiao-long, LI Hai-long, WEI You-wen, WANG Xue, YANG Xiao-yan, YANG Ning, JIN Xing. A new record of flea species in Qinghai province, China [J]. Chines Journal of Vector Biology and Control, 2013, 24(3): 239-240.
[7]	Chen Huaxin;et al. The First Detection of Anti-Xinjiang Hemorrhagic Fever Virus IgG Antibody from Sheep Sera in Qinghai Province [J]. Chines Journal of Vector Biology and Control, 1991, 2(4): 267-269.
[8]	Zhang Guangdeng;Ma Liming. Additional Studies on Some Ecological Habits of Himalayan Marmota [J]. Chines Journal of Vector Biology and Control, 1991, 2(1): 40-43.