Abstract: Objective To investigate the current status of insecticide resistance of Aedes albopictus in Wuhu city of Anhui province, China, and to provide a scientific basis for formulating standard and rational strategies for the use of public health insecticides and the prevention and control of dengue fever. Methods The larval dipping method in the sensitive baseline method was used. The insecticides to be tested were diluted by acetone into a series of concentrations, and 1 ml was taken from each concentration and was prepared into 200 ml aqueous solution. A total of 20 IV-instar larvae were placed in each solution, and the number of deaths were observed 24 hours later. SPSS 20.0 software was used to calculate median lethal concentration (LC₅₀), and the level of insecticide resistance was compared. Results A total of 2 760 Ae. albopictus larvae were tested, and the LC₅₀ values of permethrin, deltamethrin, beta-cypermethrin, propoxur, and malathion were 0.054 142, 0.007 077, 0.020 922, 0.305 849, and 0.139 400 mg/L, respectively. The resistance ratios of Ae. albopictus larvae to permethrin, deltamethrin, beta-cypermethrin, and propoxur were 27.14, 17.69, 23.27, and 0.85, respectively, suggesting moderate resistance to permethrin, deltamethrin, and beta-cypermethrin and sensitivity to propoxur. Conclusion Ae. albopictus larvae in Wuhu are moderately resistant or sensitive to four commonly used insecticides. It is recommended to reduce the use of pyrethroid insecticides and use sensitive insecticides alternatively to delay the occurrence and development of insecticide resistance.

Key words: Aedes albopictus, Insecticide resistance, Investigation

摘要： 目的 了解安徽省芜湖市白纹伊蚊的抗药性现状，为制定规范、合理的卫生杀虫剂使用及登革热防制策略提供科学依据。方法 采用敏感基线法中的幼虫浸渍法，将待测药剂用丙酮稀释成系列浓度，不同浓度分别取l ml配制成200 ml水溶液，置入20只4龄幼蚊，24 h后检查死亡数；利用SPSS 20.0软件计算半数致死浓度（LC₅₀），比较抗性水平。结果 2018年芜湖市2 760只白纹伊蚊幼蚊对氯菊酯、溴氰菊酯、高效氯氰菊酯、残杀威、马拉硫磷5种杀虫剂的LC₅₀值分别为0.054 142、0.007 077、0.020 922、0.305 849和0.139 400 mg/L，对氯菊酯、溴氰菊酯、高效氯氰菊酯、残杀威的抗性倍数分别为27.14、17.69、23.27和0.85倍，其中氯菊酯、溴氰菊酯、高效氯氰菊酯为中度抗性，残杀威为敏感。结论 芜湖市白纹伊蚊幼蚊对4种常用杀虫剂处于中抗或敏感水平，应减少拟除虫菊酯类杀虫剂的使用，轮替使用敏感型杀虫剂，以延缓抗药性的发生和发展。

关键词: 白纹伊蚊, 抗药性, 研究