An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen, China

doi:10.11853/j.issn.1003.8280.2021.01.014

Abstract

Abstract: Objective To investigate the resistance of Blattella germanica to commonly used insecticides in ten districts of Shenzhen, China and the dynamic development rules of such insecticide resistance, and to provide a scientific basis for the drug control of cockroaches and the formulation of effective prevention and control programs. Methods According to the residual film method in Cockroach insecticide resistance monitoring program (On trial) formulated by National Study Group of Cockroach Prevention and Control, 2.5 ml of 0.05% insecticide solution was placed into a 500 ml transparent wide-mouth bottle, and the wide-mouth bottle was rolled to form a uniform drug membrane. The male adults of B. germanica were put into the bottle, and the knockdown of the test insects was observed and recorded at a certain interval. SPSS 24.0 software was used to calculate median knockdown time. Results Beta-cypermethrin showed high resistance in Longgang district, with a resistance ratio of 11.34, medium resistance in Bao'an, Guangming, and Futian districts, with resistance ratios of 8.79, 8.77, and 8.07, respectively, and low resistance in the other districts. Permethrin showed high resistance in Futian, Longhua, Bao'an, Longgang, and Luohu districts, with resistance ratios of 22.26, 13.99, 14.28, 13.01, and 10.01, respectively, medium resistance in Pingshan district, with a resistance ratio of 5.51, and low resistance in the other districts. Deltamethrin showed medium resistance in Bao'an, Longgang, and Longhua districts, with resistance ratios of 7.00, 6.39, and 6.02, respectively, and low resistance in the other districts. Cypermethrin showed high resistance in Bao'an district, with a resistance ratio of 19.65, medium resistance in Futian district, with a resistance ratio of 9.42, and low resistance in the other districts. Chlorpyrifos, acephate, and propoxur showed low resistance in all districts. Conclusion Varying degrees of resistance to pyrethroid insecticides are observed in B. germanica in Shenzhen. It is suggested to stop or restrict the use of insecticides with medium and high resistance and to use chlorpyrifos, acephate, and propoxur with low resistance. At the same time, comprehensive control measures should be taken to control and delay the development and progression of insecticide resistance in B. germanica.

Key words: Blattella germanica, Insecticide, Insecticide resistance

摘要： 目的掌握深圳市10个行政区的德国小蠊对常用杀虫剂的抗药性情况，了解抗性动态和发展规律，为蜚蠊的药物防制及制定有效的防控方案提供科学依据。方法采用全国蟑螂防治学组制定的《蟑螂抗药性监测方案（试行）》规定的药膜法，取0.05%的药液2.5 ml置于500 ml透明广口瓶内，滚动广口瓶形成均匀药膜，放入德国小蠊雄性成虫，每隔一定时间观察和记录试虫的击倒情况，使用SPSS 24.0软件计算德国小蠊半数击倒时间（KT₅₀）。结果高效氯氰菊酯在龙岗区为高抗，抗性倍数为11.34倍，在宝安、光明和福田区为中抗，抗性倍数分别为8.79、8.77和8.07倍，其他地区均为低抗；氯菊酯在福田、龙华、宝安、龙岗和罗湖区为高抗，抗性倍数分别为22.26、13.99、14.28、13.01和10.01倍，在坪山区为中抗，抗性倍数为5.51倍，其他地区均为低抗；溴氰菊酯在宝安、龙岗和龙华区为中抗，抗性倍数分别为7.00、6.39和6.02倍，其他地区均为低抗；氯氰菊酯在宝安区为高抗，抗性倍数为19.65倍，在福田区为中抗，抗性倍数为9.42倍，其他地区均为低抗。而各行政区对毒死蜱、乙酰甲胺磷和残杀威均为低抗性。结论深圳市德国小蠊对拟除虫菊酯类杀虫剂产生了不同程度的抗性，建议对中、高抗性的药物进行停用或限制使用，换用低抗性的毒死蜱、乙酰甲胺磷、残杀威等药物；同时应采取综合防治措施，控制和延缓德国小蠊抗药性的产生和发展。

关键词: 德国小蠊, 杀虫剂, 抗药性

CLC Number:

HU Jing, LIU Yang, ZHANG Shao-hua, QIN Yan-min, LIANG Zhuo-nan. An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 70-73.

胡静, 刘阳, 张韶华, 秦彦珉, 梁焯南. 广东省深圳市德国小蠊对常用杀虫剂的抗药性分析[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 70-73.

References

[1] Haverinen-Shaughnessy U,Khan S,Boulafentis J,et al. Effects of educational efforts in tribal homes and schools to reduce asthma triggers,symptoms and missed school days[J]. Int J Hyg Environ Health,2020,228:113513. DOI:10.1016/j.ijheh.2020.113513.
[2] 林良强,张韶华,张燕娜,等. 深圳市病媒生物监测分析[J]. 中华卫生杀虫药械,2018,24(2):154-157. DOI:10.19821/j.1671-2781.2018.02.012.Lin LQ,Zhang SH,Zhang YN,et al. Survey on main medical vectors in Shenzhen city[J]. Chin J Hyg Insect Equip,2018,24(2):154-157. DOI:10.19821/j.1671-2781.2018.02.012.
[3] 孙俊,褚宏亮,杨维芳,等. 我国蟑螂抗药性现状与检测方法的研究[J]. 中华卫生杀虫药械,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011.01.005.Sun J,Chu HL,Yang WF,et al. Current situation and testing method of cockroach resistance in China[J]. Chin J Hyg Insect Equip,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011. 01.005.
[4] 李海龙,张桂林,党荣理,等. 应用SPSS软件进行杀虫剂的KT₅₀分析[J]. 中国媒介生物学及控制杂志,2011,22(2):155-157.Li HL,Zhang GL,Dang RL,et al. Calculation of KT₅₀ of pesticides with SPSS software[J]. Chin J Vector Biol Control,2011,22(2):155-157.
[5] 明明. 泰安市蟑螂密度季节消长及其抗药性分析[J]. 现代预防医学,2016,43(19):3476-3478.Ming M. Seasonal fluctuation of cockroach density and its resistance,Tai'an city[J]. Mod Prev Med,2016,43(19):3476-3478.
[6] 张守刚,熊丽林,孙燕群,等. 南京市蟑螂密度监测及抗药性分析[J]. 中华卫生杀虫药械,2018,24(1):20-23. DOI:10.19821/j.1671-2781.2018.01.007.Zhang SG,Xiong LL,Sun YQ,et al. Study on the density and insecticide resistance of cockroaches in Nanjing city[J]. Chin J Hyg Insect Equip,2018,24(1):20-23. DOI:10.19821/j.1671-2781.2018.01.007.
[7] 龙再浩,俞雪均,倪敏君. 宁波口岸德国小蠊对6种杀虫剂的抗药性调查[J]. 中华卫生杀虫药械,2017,23(1):88-89. DOI:10.19821/j.1671-2781.2017.01.027.Long ZH,Yu XJ,Ni MJ. Resistance of Blattella germanica to six kinds of insecticides in Ningbo port[J]. Chin J Hyg Insect Equip,2017,23(1):88-89. DOI:10.19821/j.1671-2781.2017.01.027.
[8] 徐正龙,葛伟,朱红艳. 2014年张家港市德国小蠊的抗药性调查[J]. 中华卫生杀虫药械,2015,21(5):477-478. DOI:10.19821/j.1671-2781.2015.05.012.Xu ZL,Ge W,Zhu HY. Investigation on the resistance of Blattella germanica to insecticides in Zhangjiagang city in 2014[J]. Chin J Hyg Insect Equip,2015,21(5):477-478. DOI:10.19821/j.1671-2781.2015.05.012.
[9] 苏庆盛,罗林峰,林琳,等. 深圳市德国小蠊和家蝇对常用杀虫剂的抗性研究[J]. 现代预防医学,2014,41(4):698-699,703.Su QS,Luo LF,Lin L,et al. Resistance of Blattella germanica and Musca domestica to commonly-used insecticides in Shenzhen city[J]. Mod Prev Med,2014,41(4):698-699,703.
[10] 钟积成,杨闳智,朱礼平,等. 深圳市大鹏新区德国小蠊抗药性及与抗药性相关的几种酶活性关系的研究[J]. 现代预防医学,2019,46(9):1680-1683.Zhong JC,Yang HZ,Zhu LP,et al. Chemical resistance and several correlated enzyme activities of the Blattella germanica in Dapeng new district,Shenzhen[J]. Mod Prev Med,2019,46(9):1680-1683.

[1]	ZHAO Chun-chun, ZHOU Xin-xin, LI Wen-yu, LUN Xin-chang, LIU Xiao-bo, WU Hai-xia, WANG Jun, LIU Qi-yong, MENG Feng-xia. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 30-37.
[2]	ZHENG Yu-ting, YANG Chun-mei, YANG Ming-dong, JIANG Jin-yong. Ecological and insecticide resistance surveillance of dengue vector Aedes in Yunnan border region of China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 38-43.
[3]	TU Tao-tian, XIAO Han-sen, MENG Feng-xia, LIU Qi-yong, LIU Xiao-bo, HE Ya-ming, JI Heng-qing. An investigation of the insecticide resistance of Aedes vector after emergency control of a dengue fever outbreak in Chongqing， China， 2019 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 44-47.
[4]	LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 104-107.
[5]	ZHAO Qiong-yao, QIU Xing-hui, YANG Yan, LUO Xing, SONG Xiao-ming, JIA Yong-chao. Polymorphism and resistance mutation frequency of the target genes of insecticides in Anopheles sinensis in Guangyuan of Sichuan province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(5): 541-545.
[6]	LI Jing, TONG Ying, ZENG Xiao-peng, ZHOU Xiao-jie. A comparative study of changes in water loss rate and palatability of cockroach-killing gel baits [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 560-563.
[7]	HU Yuan-feng, TAN Liang-fei. Surveillance results of cockroach density and insecticide resistance in Ezhou of Hubei province, China, 2016-2020 [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 599-603.
[8]	HE Ya-ming, JI Heng-qing, PENG Guo-xiong, XIAO Han-sen, TU Tao-tian, ZHANG Ying, YU Zhan, LIU Zong-ling, WANG Xiu-juan, LI Ming. Efficacy of Metarhizium anisopliae CQMa421 bait against cockroaches: A laboratory and field study [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 633-636.
[9]	CHEN Xiao-min, LIU Qin, ZHOU Liang-cai, WU Li-qun, BAO Ji-yong, WU Tai-ping. An investigation of resistance of Musca domestica to commonly used insecticides in Wuhan, China, 2009-2015 [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 468-471.
[10]	LI Feng, CUI Shi-lei, YAN Jing, DING Xin-yang. An investigation of the resistance of Blattella germanica to commonly used insecticides in Nanyang, Henan province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 472-474.
[11]	WANG Dan, SHI Peng, ZHAO Wen-ping, YAN Ying, ZHOU Jing-zhu, TIAN Zhen-zao, LIANG Wen-qin. Monitoring and analysis of insecticide resistance of Aedes albopictus in Xingyi and Chishui cities of Guizhou province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(3): 302-306.
[12]	LI Chun-yan, TIAN Feng-yuan, LING Yuan-nong, XIAO Chong-kun, ZHANG Fu-yan. Monitoring of the density of the dengue vector Aedes albopictus and its resistance to commonly used insecticides in Zigong of Sichuan province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(3): 307-311.
[13]	ZOU Ya-ming, LAN Ce-jie, ZHU Ding, YOU Ying-qi, LIU Yun-hua, CHEN En-pin. An analysis of resistance of Blattella germanica to commonly used insecticides in Wuxi, Jiangsu province, China, 2010-2018 [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 193-196.
[14]	LIU Yu-chao, LI Ya-lei, ZHANG Jia-wei, WANG Zong-qing. Determination of toxicity of tetramethrin and dinotefuran mixtures against Blattella germanica [J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 26-29.
[15]	ZHANG Can-ming, LI Yu-wei, CHEN Bin, HUANG Jing-wen, HUANG Zhi-yu. An analysis of the surveillance data on density and insecticide resistance of Aedes albopictus in three national surveillance sites in Fujian province, China, 2019 [J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 65-69.