Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

doi:10.11853/j.issn.1003.8280.2021.01.012

Chines Journal of Vector Biology and Control ›› 2021, Vol. 32 ›› Issue (1): 60-64.DOI: 10.11853/j.issn.1003.8280.2021.01.012

• Vector Surveillance • Previous Articles Next Articles

Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

MA Min¹, YANG Si-jia¹, LI Bao-jun², MA Xiao¹, GE Bao-xiang³, GU Feng⁴, ZHU Meng-yu⁵

1. Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China;
2. Haishu District Center for Disease Control and Prevention, Ningbo, Zhejiang 315012, China;
3. Ninghai County Center for Disease Control and Prevention, Ninghai, Zhejiang 315600, China;
4. Beilun District Center for Disease Control and Prevention, Ningbo, Zhejiang 315800, China;
5. Cixi Center for Disease Control and Prevention, Cixi, Zhejiang 315300, China

Received:2020-04-28 Online:2021-02-20 Published:2021-02-20
Supported by:
Supported by the Zhejiang Medicine Health Science and Technology Project (No. 2019KY634), Ningbo Medical Science Technology Project (No. 2017A29), Ningbo Health Branding Subject Fund Field Epidemiology (No. PPXK2018-10) and the Social Development Research Project of Haishu District Science and Technology Bureau (No. 201804F028)

浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查

马敏¹, 杨思嘉¹, 李保军², 马晓¹, 葛宝湘³, 顾锋⁴, 祝梦雨⁵

1. 宁波市疾病预防控制中心消毒与媒介生物防制所, 浙江宁波 315010;
2. 宁波市海曙区疾病预防控制中心, 浙江宁波 315012;
3. 宁海县疾病预防控制中心, 浙江宁海 315600;
4. 宁波市北仑区疾病预防控制中心, 浙江宁波 315800;
5. 慈溪市疾病预防控制中心, 浙江慈溪 315300

通讯作者: 马晓,E-mail:max@nbcdc.org.cn
作者简介:马敏,女,博士,助理研究员,主要从事媒介生物防制工作,E-mail:mamin2013@126.com
基金资助:
浙江省医药卫生科技计划项目（2019KY634）；宁波市医学科技计划项目（2017A29）；宁波市医疗卫生品牌学科现场流行病学项目（PPXK2018-10）；海曙区科技局社会发展类科研项目（201804F028）

Abstract

Abstract: Objective To investigate overwintering Aedes albopictus eggs and the dengue virus carried by the eggs in the former dengue epidemic focus in Ningbo, Zhejiang province, China, and to provide scientific evidence for dengue control. Methods During December 2018 to March 2019, standing water and sludge in containers were collected from the former dengue epidemic focus (including the core area, warning area, and monitoring area) of Haishu district and three non-epidemic areas (Beilun district, Cixi city, and Ninghai county) in Ningbo, for incubation of Ae. albopictus eggs under laboratory conditions. Real-time RT-PCR was used to detect the dengue virus carried by the eggs. The chi-square test and logistic regression analysis were used to determine the influencing factors of the positive rate of overwintering Ae. albopictus eggs. Results A total of 884 samples were collected, and the positive rate of overwintering Ae. albopictus eggs was 11.99%. There were significant differences in the positive rate of overwintering Ae. albopictus eggs between different dengue control areas (χ²=31.227, P<0.001) and between different water containers (χ²=38.044, P<0.001). According to multivariate analysis, the positive rates for the warning area, monitoring area, and non-epidemic area were 5.33 (95% confidence interval[CI]:1.16-24.49), 6.26 (95%CI:1.37-28.65), and 11.56 (95%CI:2.75-48.50) times that for the core area, respectively. The positive rates for waste tires and vegetative containers were 3.59 (95%CI:1.79-7.20) and 3.77 (95%CI:1.82-7.79) times that for stationary containers, respectively. All test results for the nucleic acid of dengue virus were negative. Conclusion The warning area and monitoring area of the former dengue epidemic focus, non-epidemic areas, waste tires, and vegetative containers are the main breeding sites for overwintering Ae. albopictus eggs. It is suggested that relevant departments should take timely measures focusing on the removal of standing water to control mosquitoes in early spring.

Key words: Aedes albopictus, Overwintering egg, Influencing factor, Dengue virus, Real-time RT-PCR

摘要： 目的了解宁波市登革热旧疫点白纹伊蚊越冬卵及其登革病毒携带情况，为登革热防控提供科学依据。方法 2018年12月至2019年3月在宁波市海曙区登革热旧疫点的核心区、警戒区和监控区，以及北仑区、慈溪市和宁海县等3个非疫点县（市、区）采集不同积水容器中的积水和积泥，在实验室条件下孵化白纹伊蚊卵，采用实时荧光定量反转录-聚合酶链式反应检测其登革病毒携带情况，并采用χ²检验和logistic回归模型分析白纹伊蚊越冬卵阳性率影响因素。结果共采集样本884份，白纹伊蚊越冬卵阳性率为11.99%。不同登革热防控区域、不同积水容器中白纹伊蚊越冬卵阳性率差异均有统计学意义（χ²=31.227，P<0.001；χ²=38.044，P<0.001）。多因素分析结果显示疫点警戒区、监控区和非疫点白纹伊蚊越冬卵阳性率分别是疫点核心区的5.33（95%CI：1.16～24.49）、6.26（95%CI：1.37～28.65）和11.56（95%CI：2.75～48.50）倍。废旧轮胎和植被性容器中白纹伊蚊越冬卵阳性率分别是固定性容器的3.59（95%CI：1.79～7.20）和3.77（95%CI：1.82～7.79）倍。登革病毒核酸检测结果均为阴性。结论登革热旧疫点警戒区、监控区和非疫点及废旧轮胎和植被性容器属当地白纹伊蚊越冬卵的主要孳生场所或孳生地，建议相关部门在早春及时采取以清除积水为主的防蚊灭蚊工作。

关键词: 白纹伊蚊, 越冬卵, 影响因素, 登革病毒, 实时荧光定量反转录-聚合酶链式反应

CLC Number:

R384.1

MA Min, YANG Si-jia, LI Bao-jun, MA Xiao, GE Bao-xiang, GU Feng, ZHU Meng-yu. Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 60-64.

马敏, 杨思嘉, 李保军, 马晓, 葛宝湘, 顾锋, 祝梦雨. 浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 60-64.

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Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查

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