A transcriptome study of Blattella germanica in response to low-temperature stress

doi:10.11853/j.issn.1003.8280.2021.01.006

Abstract

Abstract:

Objective To investigate the transcriptome of Blattella germanica in response to low-temperature stress, the functional enrichment and metabolic pathways of low-temperature response genes, and the molecular mechanism of low-temperature response in B. germanica, and to improve the understanding of the molecular mechanism of temperature adaptation in B. germanica. Methods B. germanica adults were fed in this laboratory; B. germanica adults in the 25℃ were selected as controls, and transcriptome sequencing was performed for those placed in the 4℃ for 2 hours of low-temperature treatment to identify differentially expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to analyze the functional enrichment and metabolic pathways of genes. Results The sequencing data obtained 44 589 020 non-redundant transcripts, and calculation showed that 332 genes were upregulated and 371 genes were downregulated in response to low temperature. The low-temperature response genes were enriched into 27 GO terms and 35 KEGG metabolic pathways, including carboxylic acid metabolism, stress response, carbohydrate metabolism, neurotransmitter secretion, carbonate dehydratase activity, oxidoreductase activity, lyase activity, pyruvate dehydrogenase activity, pyruvate dehydrogenase complex, water transport, abiotic stress response, channel activity, multicellular tissue process, muscle membrane, plasma membrane, steroid hormone response, organic matter response, exopeptidase, protein metabolism, "P-P hydrolysis" kinesin transmembrane transporter activity, ER-targeted protein, hydrolase activity, nutrition storage activity, transferase activity, metal carboxypeptidase activity, lipid transporter activity, and cell division. Conclusion B. germanica may respond to low-temperature stress from 27 aspects including carboxylic acid metabolism and stress response, which provides a basis for clarifying the molecular mechanisms of response and tolerance to low temperature in B. germanica.

Key words: Blattella germanica, Low temperature, Gene expression

摘要：

目的研究德国小蠊在低温胁迫下的转录组及低温应答基因的功能富集类别和代谢通路，推测其低温应答分子机制，完善德国小蠊温度适应分子机制。方法试虫为本实验室饲养，以25℃恒温培养箱环境为对照，对4℃低温处理2 h的德国小蠊成虫进行转录组测序，识别差异表达基因，利用Gene Ontology Database基因功能富集数据库和Kyoto Encyclopedia of Genes and Genomes（KEGG）基因及基因组京都信息数据库对基因功能富集和代谢途径进行分析。结果共得到44 589 020个“读序”，计算后表明低温应答上调表达基因332个、下调表达基因371个。低温应答基因功能富集为27个类别和35个KEGG代谢途径，基因分类包括羧酸代谢、胁迫应答反应、碳水化合物代谢、神经递质分泌、碳酸盐脱水酶活性、氧化还原酶活性、裂解酶活性、丙酮酸脱氢酶活性、丙酮酸脱氢酶复合物、水运输、非生物胁迫反应、通道活性、多细胞组织过程、肌膜、质膜、类固醇激素反应、有机物质应答反应、外肽酶、蛋白质代谢、“P-P水解”驱动蛋白跨膜转运蛋白活性、ER靶向蛋白质、水解酶活性、营养储存活性、转移酶活性、金属羧肽酶活性、脂质转运蛋白活性和细胞分裂等方面。结论德国小蠊可能从羧酸代谢、胁迫应答等27个方面应对低温胁迫，为完善德国小蠊温度应答和耐受分子机制提供了依据。

关键词: 德国小蠊, 低温, 基因表达

CLC Number:

R384.9

ZHANG Ya-ming, CHEN Hong-yu, ZHANG Wen-jia, WANG Yue, YU Yang, CHEN Qu. A transcriptome study of Blattella germanica in response to low-temperature stress[J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 34-37.

张雅明, 陈宏宇, 张文佳, 王玥, 于洋, 陈曲. 德国小蠊在低温胁迫下的转录组研究[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 34-37.

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