Determination of toxicity of tetramethrin and dinotefuran mixtures against Blattella germanica

doi:10.11853/j.issn.1003.8280.2021.01.004

Abstract

Abstract: Objective To determine the killing effects of tetramethrin and dinotefuran mixtures on Blattella germanica, and to screen for the formula with a synergistic effect. Methods SPSS 22.0 software was applied to determine the regression equation of toxicity and median lethal concentration (LC₅₀). The co-toxicity factor and co-toxicity coefficient were employed to evaluate the killing effects of tetramethrin and dinotefuran mixtures on B. germanica, and identify the optimal ratio of tetramethrin to dinotefuran. Results At 72 h after treatment, the LC₅₀ values of tetramethrin and dinotefuran against the male adults of B. germanica were 1 376.86 mg/L and 3.97 mg/L, respectively. When the mass ratio of tetramethrin to dinotefuran was between 231:1 and 3 123:1, the mixture had a significant synergistic effect. The optimal mass ratio was 360:1, with a co-toxicity coefficient of 190.09 and an LC₅₀ of 366.70 mg/L at 72 h after treatment. Conclusion The mixture of tetramethrin and dinotefuran shows a significant synergistic killing effect on B. germanica.

Key words: Tetramethrin, Dinotefuran, Blattella germanica, Co-toxicity coefficient, Mixture

摘要： 目的研究2种药剂复配对德国小蠊的杀蟑活性，筛选对德国小蠊防治具有增效作用的复配药剂配方。方法通过SPSS 22.0软件进行数据处理，求得毒力回归方程和半数致死浓度（LC₅₀），以共毒因子法和共毒系数法评价胺菊酯和呋虫胺对德国小蠊的联合杀蟑活性及筛选最佳配比。结果胺菊酯与呋虫胺对德国小蠊雄成虫处理后72 h的LC₅₀值分别为1 376.86和3.97 mg/L，胺菊酯与呋虫胺以质量比为231：1～3 123：1混合具有明显增效作用，最佳质量配比为360：1，对德国小蠊处理后72 h的共毒系数为190.09，LC₅₀值为366.70 mg/L。结论胺菊酯和呋虫胺复配对德国小蠊的防治具有明显的增效作用。

关键词: 胺菊酯, 呋虫胺, 德国小蠊, 共毒系数, 复配

CLC Number:

LIU Yu-chao, LI Ya-lei, ZHANG Jia-wei, WANG Zong-qing. Determination of toxicity of tetramethrin and dinotefuran mixtures against Blattella germanica[J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 26-29.

刘育超, 李娅蕾, 张佳伟, 王宗庆. 胺菊酯与呋虫胺对德国小蠊的联合毒力测定[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 26-29.

References

[1] 刘小闪. 铁路旅客列车蟑螂种群及生活习性的调查研究[J]. 铁路节能环保与安全卫生,2011,1(5):264-266. DOI:10.16374/j.cnki.issn2095-1671.2011.05.010.Liu XS. The study of cockroach population and distribution in passwenger train[J]. Railway Occup Saf Health Environ Prot,2011,1(5):264-266. DOI:10.16374/j.cnki.issn2095-1671. 2011.05.010.
[2] 周良才,刘小泉,包继永,等. 武汉市德国小蠊对5种杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2016,27(1):75-76. DOI:10.11853/j.issn.1003.4692.2016.01.024.Zhou LC,Liu XQ,Bao JY,et al. Investigation on resistance of Blattella germanica against five insecticides in Wuhan[J]. Chin J Vector Biol Control,2016,27(1):75-76. DOI:10.11853/j.issn. 1003.4692.2016.01.024.
[3] 李树双,项冬,张丽萍,等. 唐山市德国小蠊对常用杀虫剂的抗药性调查[J]. 医学动物防制,2016,32(12):1390-1391. DOI:10.7629/yxdwfz201612029.Li SS,Xiang D,Zhang LP,et al. Resistance of Blattella germanica to common insecticides in Tangshan[J]. J Med Pest Control,2016,32(12):1390-1391. DOI:10.7629/yxdwfz201612029.
[4] 涂涛田,季恒青,陶晓颖,等. 重庆市德国小蠊对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2017,28(3):226-229. DOI:10.11853/j.issn.1003.8280.2017.03.007.Tu TT,Ji HQ,Tao XY,et al. Resistance of Blattella germanica to commonly used insecticides in Chongqing[J]. Chin J Vector Biol Control,2017,28(3):226-229. DOI:10.11853/j.issn.1003. 8280.2017.03.007.
[5] 邹羽芯. 胺菊酯的水生毒性及微生物降解[D]. 杭州:浙江工业大学,2012.Zou YX. The aquatic toxicity and microbial degra dation of tetramethrin[D]. Hangzhou:Zhejiang University of Technology,2012.
[6] Bloomquist JR. Ion channels as targets for insecticides[J]. Ann Rev Entomol,1996,41:163-190. DOI:10.1146/annurev.en.41. 010196.001115.
[7] 刘安昌,张良,谭珍友,等. 新型烟碱类杀虫剂呋虫胺的合成研究[J]. 世界农药,2009,31(2):22-23,30. DOI:10.3969/j.issn.1009-6485.2009.02.006.Liu AC,Zhang L,Tan ZY,et al. Study of synthetic process of novel neonicotinoid dinotefuran[J]. World Pestic,2009,31(2):22-23,30. DOI:10.3969/j.issn.1009-6485.2009.02.006.
[8] 赵聪,杨文革,胡永红,等. 呋虫胺及其中间体的合成方法[J]. 现代农药,2009,8(2):13-15. DOI:10.3969/j.issn.1671-5284.2009.02.003.Zhao C,Yang WG,Hu YH,et al. Review on synthetic methods of dinotefuran and related intermediates[J]. Mod Agrochemicals,2009,8(2):13-15. DOI:10.3969/j.issn.1671-5284.2009. 02.003.
[9] 曾雪珍,张冬梅,谢素雯. 漫话呋虫胺[J]. 江西化工,2014(4):6-11. DOI:10.3969/j.issn.1008-3103.2014.04.002.Zeng XZ,Zhang DM,Xie SW. Rambling dinotefuran[J]. Jiangxi Chem Ind,2014(4):6-11. DOI:10.3969/j.issn.1008-3103. 2014.04.002.
[10] 杨寿海,徐盈,陈志忠,等. 一种含呋虫胺和噻虫嗪的增效杀虫组合物及其应用:中国,CN201110320884.5[P]. 2013-04-24.Yang SH,Xu Y,Chen ZZ,et al. A synergistic insecticidal composition containing dinotefuran and thiamethoxam,and application thereof:CN,CN201110320884.5[P]. 2013-04-24.
[11] Mansour NA,Eldefrawi ME,Toppozada A,et al. Toxicological studies on the Egyptian cotton leaf worm,Prodenia litura. Ⅵ. Potentiation and antagonism of organophosphorus and carbamate insecticides[J]. J Econ Entomol,1966,59(2):307-311. DOI:10.1093/jee/59.2.307.
[12] Sun YP,Johnson ER. Analysis of joint action of insecticides against house flies[J]. J Econ Entomol,1960,53(5):887-892. DOI:10.1093/jee/53.5.887.
[13] 陈立,徐汉虹,李云宇,等. 农药复配最佳增效配方筛选方法的探讨[J]. 植物保护学报,2000,27(4):349-354. DOI:10.3321/j.issn:0577-7518.2000.04.012.Chen L,Xu HH,Li YY,et al. Study on the screening method for optimal synergetic ratio of combined pesticides[J]. Acta Phytophyl Sin,2000,27(4):349-354. DOI:10.3321/j.issn:0577-7518.2000.04.012.

[1]	LI Jing, TONG Ying, ZENG Xiao-peng, ZHOU Xiao-jie. A comparative study of changes in water loss rate and palatability of cockroach-killing gel baits [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 560-563.
[2]	HU Yuan-feng, TAN Liang-fei. Surveillance results of cockroach density and insecticide resistance in Ezhou of Hubei province, China, 2016-2020 [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 599-603.
[3]	HE Ya-ming, JI Heng-qing, PENG Guo-xiong, XIAO Han-sen, TU Tao-tian, ZHANG Ying, YU Zhan, LIU Zong-ling, WANG Xiu-juan, LI Ming. Efficacy of Metarhizium anisopliae CQMa421 bait against cockroaches: A laboratory and field study [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 633-636.
[4]	LI Feng, CUI Shi-lei, YAN Jing, DING Xin-yang. An investigation of the resistance of Blattella germanica to commonly used insecticides in Nanyang, Henan province, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 472-474.
[5]	ZOU Ya-ming, LAN Ce-jie, ZHU Ding, YOU Ying-qi, LIU Yun-hua, CHEN En-pin. An analysis of resistance of Blattella germanica to commonly used insecticides in Wuxi, Jiangsu province, China, 2010-2018 [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 193-196.
[6]	HU Jing, LIU Yang, ZHANG Shao-hua, QIN Yan-min, LIANG Zhuo-nan. An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 70-73.
[7]	LIU Yao, LIU Hong-xia, LENG Pei-en, XU Jin-qiu, ZHU Jiang, FAN Ming-qiu, ZHOU Yi-bin. Laboratory efficacy against Blattella germanica of four cockroach-killing gel baits containing different effective constituents [J]. Chines Journal of Vector Biology and Control, 2020, 31(5): 559-564.
[8]	LIU Yu-chao, LIU Jin-lin, LI Ya-lei, WANG Zong-qing. Study on indoor rearing technique of Blattella germanica [J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 474-476.
[9]	WANG Fei, JIANG Lu, HUANG Hui-chang, LIU Hong-xia, CHEN Dao-yong. Resistance of main vectors to commonly used insecticides in Hongkou district, Shanghai, China, 2015-2019 [J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 143-147.
[10]	CHEN Hong-yu, ZHANG Ya-ming, ZHANG Wen-jia, YU Yang, GAO Xiao-li, WANG Yue. Effect of high temperature on gene expression of Blattella germanica [J]. Chines Journal of Vector Biology and Control, 2020, 31(1): 62-65.
[11]	HUNAG Zhen-dong, WAN Qing, XUE Zhi-jing, ZHANG Rui-ling, ZHANG Zhong. Isolation and identification of culturable aerobic bacteria from the intestines of Blattella germanica and the activity of digestive enzymes produced by these bacteria [J]. Chines Journal of Vector Biology and Control, 2019, 30(4): 409-413.
[12]	SONG Xiao, CHENG Peng, LYU Ye-yuan, SHI Qi-qi, LIU Hong-mei, WANG Hai-fang, LIU Li-juan, GUO Xiu-xia, ZHANG Chong-xing, ZHAO Yu-qiang, WANG Huai-wei, GONG Mao-qing. Efficacy of chemical insecticide mixture and biological insecticides against Culex pipiens pallens in Jining, China [J]. Chines Journal of Vector Biology and Control, 2019, 30(3): 296-299.
[13]	WANG Yue, SHI hua, ZHANG Ya-ming, ZHANG Wen-jia, CHEN Hong-yu, ZHANG Dan, GAO Xiao-li, YANG Zhen-zhou. The effect evaluation of the high-temperature steam equipment in killing indoor Blattella germanica [J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 100-102.
[14]	TAN Liang-fei, HU Meng-jia, XIONG Jin-feng, YAO Xuan, ZHOU Liang-cai, GAO Yan, GAO Xiong, NI Jian-wei, ZHU Bin-bin, JIA Qian, DU Zhen-bao, HAO Hai-bo, HU Yuan-feng, LI Guo-hui. Resistance of Blattella germanica to commonly used insecticides in Hubei province [J]. Chines Journal of Vector Biology and Control, 2018, 29(2): 165-167.
[15]	WEI Xu-qiang, ZHOU Xiao-jie. Study on insecticide resistance dynamics to beta-cypermethrin in Blattella germanica collected from different districts, Beijing [J]. Chines Journal of Vector Biology and Control, 2018, 29(1): 27-29.