An investigation of a dengue outbreak in a rural area in Hangzhou, Zhejiang province, China, 2018

doi:10.11853/j.issn.1003.8280.2020.04.018

Abstract

Abstract: Objective To investigate a dengue outbreak in a rural area in Hangzhou, Zhejiang province, China, and to provide a scientific basis for dengue control and prevention in rural areas. Methods The descriptive epidemiological method was used to analyze the epidemiological characteristics of the dengue outbreak in rural Hangzhou in September 2018. The serum samples from the dengue patients were collected to detect viral nucleic acids and antibodies, and the viral nucleic acids were extracted for E gene amplification and sequencing to construct a phylogenetic tree. The density of mosquitoes was monitored by the Breteau index. Results There were 13 local cases of dengue reported, and the attack rate was 0.13%. The patients were aged 30 to 79 years, mainly over 50 years. Most of them were farmers. Five virus isolates were identified as dengue virus type I by sequence analysis, highly homologous to the type 1 dengue viruses from Southeast Asian countries including Thailand and Myanmar. Conclusion Type 1 dengue virus is responsible for this local outbreak of dengue in rural Hangzhou, and possibly comes from Southeast Asian countries. Relevant authorities should further strengthen monitoring of imported dengue cases.

Key words: Dengue fever, Outbreak, Epidemiological investigation

摘要： 目的对杭州市一起农村登革热暴发疫情开展调查与分析，为制订农村登革热防控措施提供科学依据。方法采用描述性流行病学方法分析2018年9月杭州市农村一起登革热的流行病学特征；采集患者血清进行登革病毒核酸和抗体检测，提取病毒核酸后扩增E基因并测序，构建进化树；采用布雷图指数（BI）法进行蚊媒密度监测。结果该起疫情共报告登革热本地病例13例，罹患率为0.13%；其中，发病年龄最小30岁，最大79岁，以50岁以上人群为主；职业分布以农民居多。5株登革病毒株经序列分析，均属登革病毒1型，与泰国和缅甸等东南亚国家登革病毒1型同源性高。结论该起农村登革热本地暴发疫情由登革病毒1型引起，传染源可能来自东南亚国家，相关部门应进一步加强登革热输入病例监测。

关键词: 登革热, 暴发疫情, 流行病学调查

CLC Number:

R373.3⁺3

SUN Zhou, CHEN Jun-fang, ZHU Jun-wei, KAO Qing-jun, HUANG Ren-jie, WANG Hao-qiu. An investigation of a dengue outbreak in a rural area in Hangzhou, Zhejiang province, China, 2018[J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 465-468.

孙昼, 陈珺芳, 竹军伟, 考庆君, 黄仁杰, 汪皓秋. 杭州市2018年一起农村登革热暴发疫情调查研究[J]. 中国媒介生物学及控制杂志, 2020, 31(4): 465-468.

References

[1] Shepard DS,Undurraga EA,Halasa YA,et al. The global economic burden of dengue:a systematic analysis[J]. Lancet Infect Dis,2016,16(8):935-941. DOI:10.1016/S1473-3099(16)00146-8.
[2] Sun JM,Lu L,Wu HX,et al. Epidemiological trends of dengue in mainland China,2005-2015[J]. Int J Infect Dis,2017,57:86-91. DOI:10.1016/j.ijid.2017.02.007.
[3] Guzman MG,Harris E. Dengue[J]. Lancet,2015,385(9966):453-465. DOI:10.1016/S0140-6736(14)60572-9.
[4] Sun JF,Zhang H,Tan QQ,et al. The epidemiological characteristics and molecular phylogeny of the dengue virus in Guangdong,China,2015[J]. Sci Rep,2018,8:9976. DOI:10.1038/s41598-018-28349-2.
[5] 林春萍,孔超,贺嫦悠,等. 浙江东南沿海一起登革热暴发疫情调查处置效果分析[J]. 浙江医学,2018,40(9):993-995,1003. DOI:10.12056/j.issn.1006-2785.2017.40.9.2018-869. Lin CP,Kong C,He CY,et al. Analysis on the effect of investigation and treatment of an outbreak of dengue fever in the southeast coast of Zhejiang province[J]. Zhejiang Med J,2018,40(9):993-995,1003. DOI:10.12056/j.issn.1006-2785.2017.40.9.2018-869.
[6] Yu H,Kong QX,Wang J,et al. Multiple lineages of dengue virus serotype 2 cosmopolitan genotype caused a local dengue outbreak in Hangzhou,Zhejiang province,China,in 2017[J]. Sci Rep,2019,9:7345. DOI:10.1038/s41598-019-43560-5.
[7] Waggoner JJ,Abeynayake J,Balassiano I,et al. Multiplex nucleic acid amplification test for diagnosis of dengue fever,malaria,and leptospirosis[J]. J Clin Microbiol,2014,52(6):2011-2018. DOI:10.1128/JCM.00341-14.
[8] 牟笛,李昱,殷文武,等. 中国登革热病例流行病学特征及传播媒介监测数据分析[J]. 国际病毒学杂志,2016,23(3):177-180,196. DOI:10.3760/cma.j.issn.1673-4092.2016.03.009. Mu D,Li Y,Yin WW,et al. Analysis on the epidemiological characteristics of dengue fever in China[J]. Int J Virol,2016,23(3):177-180,196. DOI:10.3760/cma.j.issn.1673-4092.2016.03.009.
[9] 牟笛,何泱霓,陈秋兰,等. 我国2016年登革热输入和本地病例流行病学特征比较[J]. 疾病监测,2017,32(3):184-189. DOI:10.3784/j.issn.1003-9961.2017.03.004. Mu D,He YN,Chen QL,et al. Comparison of epidemiological features between imported and indigenous dengue fever cases in China[J]. Dis Surveil,2017,32(3):184-189. DOI:10.3784/j.issn.1003-9961.2017.03.004.
[10] 孙昼,黄仁杰,任晓宾,等. 杭州市2004-2017年登革热输入病例的流行特征[J]. 国际流行病学传染病学杂志,2019,46(1):74-77. DOI:10.3760/cma.j.issn.1673-4149.2019.01.017. Sun Z,Huang RJ,Ren XB,et al. Epidemiological analysis on dengue fever imported cases in Hangzhou,2004-2017[J]. Int J Epidemiol Infect Dis,2019,46(1):74-77. DOI:10.3760/cma.j.issn.1673-4149.2019.01.017.
[11] 随海田,马小军,杨益昌,等. 福建省福州市鼓楼区一起登革热暴发疫情现场调查报告[J]. 中国人兽共患病学报,2016,32(12):1131-1134. DOI:10.3969/j.issn.1002-2694.2016.012.017. Sui HT,Ma XJ,Yang YC,et al. Dengue fever outbreak in a district of Fuzhou,Fujian,China,2015[J]. Chin J Zoonoses,2016,32(12):1131-1134. DOI:10.3969/j.issn.1002-2694.2016.012.017.
[12] 叶双岚,曹伟强,罗雷,等. 2006-2017年广州市白云区登革热暴发疫情流行特征分析[J]. 华南预防医学,2018,44(6):549-551. DOI:10.13217/j.scjpm.2018.0549. Ye SL,Cao WQ,Luo L,et al. Epidemiological characteristics of dengue fever outbreak in Baiyun district,Guangzhou,2006-2017[J]. South China J Prev Med,2018,44(6):549-551. DOI:10.13217/j.scjpm.2018.0549.
[13] 范建华,冯云,朱进,等. 2017年云南省西双版纳州登革1型病毒暴发疫情的调查研究[J]. 疾病监测,2019,34(5):427-434. DOI:10.3784/j.issn.1003-9961.2019.05.013. Fan JH,Feng Y,Zhu J,et al. An outbreak of dengue serotype 1 virus in Xishuangbanna prefecture of Yunnan province,China,2017[J]. Dis Surveil,2019,34(5):427-434. DOI:10.3784/j.issn.1003-9961.2019.05.013.
[14] 刘杰,吴俊秋,李剑森,等. 广东省一起工地登革热暴发疫情调查处置[J]. 中国媒介生物学及控制杂志,2016,27(1):80-83. DOI:10.11853/j.issn.1003.4692.2016.01.026. Liu J,Wu JQ,Li JS,et al. Investigation and response of the dengue outbreak on a construction site in Guangdong province[J]. Chin J Vector Biol Control,2016,27(1):80-83. DOI:10.11853/j.issn.1003.4692.2016.01.026.
[15] 周瑞青,朱海城,高淑萍. 河源市某建筑工地一起登革热暴发疫情分析[J]. 热带病与寄生虫学,2019,17(1):33-35. DOI:10.3969/j.issn.1672-2302.2019.01.009. Zhou RQ,Zhu HC,Gao SP. Analysis of an outbreak of dengue fever in a construction site in Heyuan[J]. J Trop Dis Parasitol,2019,17(1):33-35. DOI:10.3969/j.issn.1672-2302.2019.01.009.
[16] 羊晶晶,陈敏红,王瀚炜,等. 福州市2016年登革热暴发疫情的流行特征分析[J]. 中国热带医学,2017,17(8):795-797,805. DOI:10.13604/j.cnki.46-1064/r.2017.08.13. Yang JJ,Chen MH,Wang HW,et al. Analysis of epidemiological characteristics of dengue fever outbreaks in Fuzhou,2016[J]. China Trop Med,2017,17(8):795-797,805. DOI:10.13604/j.cnki.46-1064/r.2017.08.13.
[17] 陈恩富,郭颂,黄文忠,等. 农村蚊虫防制和"无蚊村"建设评价指标体系[J]. 预防医学,2019,31(3):217-220. DOI:10.19485/j.cnki.issn2096-5087.2019.03.001. Chen EF,Guo S,Huang WZ,et al. The mosquito control and evaluation system of "mosquito-free village" construction in rural areas[J]. J Prev Med,2019,31(3):217-220. DOI:10.19485/j.cnki.issn2096-5087.2019.03.001.
[18] 吴红照,刘营,黄文忠,等. 浙江省浦江县"无蚊村"建设的效果评价[J]. 中国媒介生物学及控制杂志,2018,29(3):283-286. DOI:10.11853/j.issn.1003.8280.2018.03.016. Wu HZ,Liu Y,Huang WZ,et al. Evaluation on construction of "mosquito-free village" in Pujiang county,Zhejiang,China[J]. Chin J Vector Biol Control,2018,29(3):283-286. DOI:10.11853/j.issn.1003.8280.2018.03.016.
[19] 刘永华,尹小雄,杨召兰,等. 云南省瑞丽市2013年登革热暴发的流行病学分析[J]. 中国媒介生物学及控制杂志,2014,25(6):524-526. DOI:10.11853/j.issn.1003.4692.2014.06.010. Liu YH,Yin XX,Yang ZL,et al. Epidemiological study on an outbreak of dengue fever in Ruili,Dehong prefecture of Yunnan province,China[J]. Chin J Vector Biol Control,2014,25(6):524-526. DOI:10.11853/j.issn.1003.4692.2014.06.010.
[20] 张栋梁,王蓉,易波,等. 一起登革热暴发疫情调查[J]. 预防医学,2019,31(8):844-847. DOI:10.19485/j.cnki.issn2096-5087.2019.08.024. Zhang DL,Wang R,Yi B,et al. Investigation on an outbreak of dengue fever[J]. J Prev Med,2019,31(8):844-847. DOI:10.19485/j.cnki.issn2096-5087.2019.08.024.