Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China

doi:10.11853/j.issn.1003.8280.2020.04.009

Chines Journal of Vector Biology and Control ›› 2020, Vol. 31 ›› Issue (4): 426-429.DOI: 10.11853/j.issn.1003.8280.2020.04.009

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Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China

ZHANG Lin¹, WEN Shu², HOU Xue-xia¹, LI Sha³, YANG Xiao-na¹, CHEN Ting³, MIAO Guang-qing¹, FU Xiao-ying³, HAO Qin¹, ZHU Xiong³

1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 People's Hospital of Qiongzhong Li and Miao Autonomous County;
3 People's Hospital of Sanya

Received:2020-02-05 Online:2020-08-20 Published:2020-08-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404-006-003, 2018ZX10101002), Key R&D Project of Hainan Province (No. ZDYF2017163) and Scientific Research Project of Health and Family Planning of Hainan Province (No. 16A200051,19A200039)

海南省琼中地区寄生蜱携带伯氏疏螺旋体的检测及基因分型研究

张琳¹, 文书², 侯学霞¹, 李沙³, 杨小娜¹, 陈婷³, 苗广青¹, 符晓莹³, 郝琴¹, 朱雄³

1 中国疾病预防控制中心传染病预防控制所莱姆病室, 传染病预防控制国家重点实验室, 北京 102206;
2 海南省琼中黎族苗族自治县人民医院, 海南琼中 572900;
3 海南省三亚人民医院, 海南三亚 572000

通讯作者: 郝琴,Email:haoqin@icdc.cn;朱雄,Email:zhuxiong6@163.com
作者简介:张琳,女,博士,副研究员,从事莱姆病检测技术与空间生态学研究工作,Email:zhanglin@icdc.cn;文书,女,副主任检验技师,从事临床病原微生物研究工作,Email:jykwenshu@126.com
基金资助:
“十三五”国家科技重大专项（2017ZX10303404-006-003，2018ZX10101002）；海南省重点研发计划项目课题基金（ZDYF2017163）；海南省卫计行业科研项目（16A200051，19A200039）

Abstract

Abstract: Objective To investigate the infection status of tick vectors with Borrelia burgdorferi in Qiongzhong area of Hainan province, China, and to provide a scientific basis for the local prevention and control of Lyme disease. Methods A total of 120 parasitic ticks were collected from cattle in Qiongzhong area. Polymerase chain reaction (PCR) was performed to amplify the 16S rRNA gene of the ticks, and sequence analysis was performed to identify tick species. Nested-PCR was used to determine the infection rate of B. burgdorferi in ticks, and a homology analysis was performed based on the sequencing results to determine the genotypes of B. burgdorferi. Results All of the 120 ticks were identified as Rhipicephalus microplus, among which 44 tested positive for B. burgdorferi, with a positive rate of 36.67%. The homology analysis indicated that the R. microplus in Qiongzhong area could be infected with B. burgdorferi of various genotypes, including B. afzelii (56.82%), B. yangtze (25.00%), and B. garinii (18.18%). Conclusion The parasitic ticks in Qiongzhong area of Hainan province were infected with B. burgdorferi of various genotypes, with a high infection rate and risk of Lyme disease transmission. The local investigation and surveillance of Lyme disease in humans and hosts should be enhanced.

Key words: Tick, Borrelia burgdorferi, Bioinformatic analysis, Qiongzhong area

摘要： 目的了解海南省琼中地区媒介蜱伯氏疏螺旋体的感染状况，为当地莱姆病的防控提供科学依据。方法在海南省琼中地区收集牛寄生蜱120只，应用普通PCR法扩增16S rRNA基因并测序分析，鉴定蜱种。应用巢式PCR方法，检测蜱的伯氏疏螺旋体带菌率，并对测序结果进行同源性比较以确定基因型。结果本次海南省琼中地区牛寄生蜱经鉴定均为微小牛蜱。在120只蜱标本中，有44只蜱伯氏疏螺旋体检测阳性，阳性率为36.67%。同源性分析表明，琼中地区的微小牛蜱可感染多种基因型的伯氏疏螺旋体，包括Borrelia afzelii（56.82%）、B.yangze（25.00%）和B.garinii（18.18%）基因型。结论琼中地区微小牛蜱中携带多种基因型的伯氏疏螺旋体，并且带菌率较高，有传播莱姆病的风险，应进一步加强当地人群和宿主的莱姆病调查和评估。

关键词: 蜱, 伯氏疏螺旋体, 生物信息分析, 琼中地区

CLC Number:

R377

ZHANG Lin, WEN Shu, HOU Xue-xia, LI Sha, YANG Xiao-na, CHEN Ting, MIAO Guang-qing, FU Xiao-ying, HAO Qin, ZHU Xiong. Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China[J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 426-429.

张琳, 文书, 侯学霞, 李沙, 杨小娜, 陈婷, 苗广青, 符晓莹, 郝琴, 朱雄. 海南省琼中地区寄生蜱携带伯氏疏螺旋体的检测及基因分型研究[J]. 中国媒介生物学及控制杂志, 2020, 31(4): 426-429.

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Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China

海南省琼中地区寄生蜱携带伯氏疏螺旋体的检测及基因分型研究

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