Research progress in MinION sequencing technology and its application

doi:10.11853/j.issn.1003.8280.2020.03.029

Abstract

Abstract: In 2014, the nanopore sequencer MinION was released by Oxford Nanopore Technologies (ONT), representing the birth of a novel sequencing technology. The device is extremely portable and can carry out target detection in the field through a laptop. It is characterized by single-molecule sequencing, real-time monitoring of sequencing data, ultra-long read lengths, and low cost. It has been applied to the identification of microbial populations, whole genome sequencing, discovery of new species, etc. Although the technology has made impressive achievements in recent years, there are also some problems and challenges. This review mainly summarizes the technical characteristics and development fields of the MinION sequencer, and discusses its development prospects.

Key words: Nanopore sequencer MinION, Single-molecule sequencing, Application

摘要： 牛津纳米孔技术公司（ONT）的纳米孔测序仪（MinION）于2014年发布，代表了1种新型测序技术的诞生。该设备简单便携，可通过1台笔记本电脑在现场完成目标检测，它的特点是单分子测序、测序数据实时监控、超长读长和低成本等，目前已被应用于微生物种群鉴定、全基因组测序、新物种发现等方面。尽管该技术近几年取得了令人可喜的成绩，但同时也出现了一些问题和挑战，该文综述了MinION的技术特征及开发应用，并探讨了其发展前景。

关键词: 纳米孔测序仪, 单分子测序, 应用

CLC Number:

YAN Dong-ming, ZHAO Ning, ZHAO Chun-chun, WANG Jun, SONG Xiu-ping, ZHU Cai-ying, LIU Qi-yong, ZHANG Qin-feng. Research progress in MinION sequencing technology and its application[J]. Chines Journal of Vector Biology and Control, 2020, 31(3): 380-384.

闫冬明, 赵宁, 赵春春, 王君, 宋秀平, 朱彩英, 刘起勇, 张钦凤. 纳米孔测序技术研究进展[J]. 中国媒介生物学及控制杂志, 2020, 31(3): 380-384.

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