Expression level of olfactory receptor 10 gene in Aedes albopictus before and after blood sucking

doi:10.11853/j.issn.1003.8280.2020.03.017

Chines Journal of Vector Biology and Control ›› 2020, Vol. 31 ›› Issue (3): 331-334.DOI: 10.11853/j.issn.1003.8280.2020.03.017

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Expression level of olfactory receptor 10 gene in Aedes albopictus before and after blood sucking

HUANG Xiao-dan¹, YANG Lin-lin², LIU Lu-hong², CHENG Peng¹, LIU Hong-mei¹

1 Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, Shandong Prvoince, China;
2 Jining City Center for Disease Control and Prevention

Received:2019-12-21 Online:2020-06-20 Published:2020-06-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81702034), Shandong Province Key Research and Development Program of Shandong Province (No. 2018GSF118040, 2018GSF118092, 2019GSF111006), China Postdoctoral Science Foundation (No. 2017M622733), and the Innovation Project of Shandong Academy of Medical Sciences

白纹伊蚊吸血前后气味受体OR10基因表达水平的比较研究

黄晓丹¹, 杨琳琳², 刘鲁宏², 程鹏¹, 刘宏美¹

1 山东省寄生虫病防治研究所, 山东第一医科大学(山东省医学科学院), 山东济宁 272033;
2 济宁市疾病预防控制中心, 山东济宁 2720332

通讯作者: 刘宏美,Email:liuhmm163@163.com
作者简介:黄晓丹,女,助理研究员,主要从事寄生虫防治工作,Email:hd19840502@126.com;杨琳琳,女,主管医师,主要从事病媒生物防制工作,Email:280392403@qq.com
基金资助:
国家自然科学基金（81702034）；山东省重点研发计划（2018GSF118040，2018GSF118092，2019GSF111006）；中国博士后科学基金（2017M622733）；山东省医学科学院医药卫生科技创新工程

Abstract

Abstract: Objective The olfactory system plays an important role in mosquito behaviors such as blood sucking and oviposition site selection. This study compared the expression level of odorant receptor 10 gene (OR10) in Aedes albopictus before and after blood sucking. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify the OR10 gene, and quantitative real-time PCR was used to determine the expression levels of OR10 gene in male Ae. albopictus, and female Ae. albopictus before and after blood sucking. Results The open reading frame of the OR10 gene in Ae. albopictus obtained by amplification was 1 128 bp in length, encoding 376 amino acids. The amino acid sequence had an identity of 86.13% and 69.69%, respectively, with that in Ae. aegypti and Anopheles gambiae. The results of quantitative real-time PCR showed that male Ae. albopictus had the lowest expression level of OR10 gene of 1; female Ae. albopictus had a higher expression level (1.535±0.274) than male Ae. albopictus; female Ae. albopictus had the highest expression level of OR10 gene (2.284±0.203) after blood sucking; the expression level of OR10 gene in female Ae. albopictus changed significantly before and after blood sucking (F=32.067, P=0.001). Conclusion The expression level of OR10 in Ae. albopictus changes significantly before and after blood sucking, which further proves that OR10 may be involved in the mosquito behavior of oviposition site selection after blood sucking.

Key words: Aedes albopictus, Odorant receptor 10 gene, Oviposition sites

摘要： 目的嗅觉系统在蚊虫吸血和寻找产卵地等活动中发挥着十分重要的作用。该研究对白纹伊蚊吸血前后气味受体（odorant receptors，ORs）OR10基因的表达水平进行探讨。方法通过反转录-聚合酶链式反应（RT-PCR）方法扩增白纹伊蚊OR10，采用荧光定量PCR检测雄蚊、吸血雌蚊和未吸血雌蚊OR10基因的表达量。结果扩增获得OR10基因的开放阅读框全长为1 128 bp，编码376个氨基酸。与埃及伊蚊和冈比亚按蚊的OR10基因的氨基酸序列一致性分别为86.13%和69.69%。荧光定量PCR发现雄蚊OR10基因的表达量最低为1，雌蚊OR10基因的表达量为1.535±0.274，高于雄蚊，吸血后的雌蚊OR10基因的表达量最高，为2.284±0.203，且未吸血与吸血后的雌蚊OR10基因的表达量差异有统计学意义（F=32.067，P=0.001）。结论白纹伊蚊吸血前后气味受体OR10基因表达水平差异有统计学意义，进一步辅证了OR10蛋白可能参与蚊虫吸血后寻找产卵地的行为。

关键词: 白纹伊蚊, OR10基因, 产卵地

CLC Number:

R384.1

HUANG Xiao-dan, YANG Lin-lin, LIU Lu-hong, CHENG Peng, LIU Hong-mei. Expression level of olfactory receptor 10 gene in Aedes albopictus before and after blood sucking[J]. Chines Journal of Vector Biology and Control, 2020, 31(3): 331-334.

黄晓丹, 杨琳琳, 刘鲁宏, 程鹏, 刘宏美. 白纹伊蚊吸血前后气味受体OR10基因表达水平的比较研究[J]. 中国媒介生物学及控制杂志, 2020, 31(3): 331-334.

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Expression level of olfactory receptor 10 gene in Aedes albopictus before and after blood sucking

白纹伊蚊吸血前后气味受体OR10基因表达水平的比较研究

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