Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China

doi:10.11853/j.issn.1003.8280.2020.01.009

Abstract

Abstract: Objective To monitor and identify the ticks carried by wool imported from Kyrgyz Republic to China, and to prevent the transmission of harmful vectors through international trade. Methods Sixteen batches of wool imported from Kyrgyz Republic at Turgart port, Xinjiang Uygur Autonomous Region, from September 2017 to June 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene, 18S rRNA gene, and cytochrome C oxidase subunitⅠgene were amplified and sequenced to construct a phylogenetic tree using MEGA 7.0 software for molecular biological identification. Results Sixteen batches of wool were imported from Kyrgyz Republic, and ticks were detected in 11 batches of the imported wool. A total of 39 ticks were detected, including 1 live tick. They belonged to 5 species of 4 genera in 2 families as identified by morphological and molecular phylogenetic analysis:Ornithodoros lahorensis, Hyalomma asiaticum, Dermacentor marginatus, Haemaphysalis punctata, and H. sulcata. There were 30 ticks of H. sulcata, as the predominant species (76.9% of the total number) of intercepted ticks carried by the wool imported from Kyrgyz Republic. The live tick was a nymph of O. lahorensis. Conclusion The intercepted ticks are probably detected in wool imported from Kyrgyz Republic, which are mainly H. sulcata. The live nymph of O. lahorensis was intercepted for the first time. Therefore, the quarantine of ticks carried by wool imported from Kyrgyz Republic should be strengthened to prevent vector invasion and transmission of harmful diseases.

Key words: Kyrgyz Republic, Wool, Ornithodoros lahorensis, Haemaphysalis sulcata, Identification

摘要： 目的为防止有害媒介生物通过国际贸易传入我国，对进口羊毛进行蜱监测与鉴定。方法采集2017年9月至2018年6月新疆维吾尔自治区吐尔尕特口岸从吉尔吉斯共和国进口的16批次羊毛，对其进行蜱检疫。将检出的蜱进行形态学鉴定，通过提取其基因组DNA，扩增线粒体16S rRNA基因、18S rRNA基因以及细胞色素C氧化酶亚基Ⅰ（COⅠ）基因并测序，利用MEGA 7.0软件构建系统发育树对其进行分子生物学鉴定。结果从吉尔吉斯共和国进口的16批次羊毛中有11批次检出蜱。共检出蜱39只，其中活体1只。经形态学和分子生物学鉴定，隶属2科4属5种，分别为拉合钝缘蜱、亚洲璃眼蜱、边缘革蜱、刻点血蜱和具沟血蜱。其中具沟血蜱30只，占截获蜱总数的76.9%，为吉尔吉斯共和国进口羊毛携带蜱的优势种类；活体蜱为拉合钝缘蜱若蜱。结论吉尔吉斯共和国进口羊毛携带蜱的概率较高，以具沟血蜱为主，且首次从进口羊毛中检出拉合钝缘蜱若蜱活体。因此，从吉尔吉斯共和国进口羊毛要加强对蜱的检疫，防止病媒生物入侵，严防疫病传入。

关键词: 吉尔吉斯共和国, 羊毛, 拉合钝缘蜱, 具沟血蜱, 鉴定

CLC Number:

R384.4

WANG Ke-ke, HE Yu-ping, WANG Qiang, LIN Ying-zheng, CHEN Rui-hua, WANG Yan, SHI Qian, DENG Yao-hua, XU Xin-feng, CHEN Zhi-fei, LI Shu-qing. Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China[J]. Chines Journal of Vector Biology and Control, 2020, 31(1): 41-45.

王科珂, 何宇平, 王强, 林颖峥, 陈瑞花, 王艳, 史茜, 邓耀华, 徐新峰, 陈志飞, 李树清. 吉尔吉斯共和国进口羊毛携带蜱的监测与鉴定[J]. 中国媒介生物学及控制杂志, 2020, 31(1): 41-45.

References

[1] Zhang YK,Zhang XY,Liu JZ. Ticks (Acari:Ixodoidea) in China:geographical distribution,host diversity,and specificity[J]. Arch Insect Biochem Physiol,2019,102(3):e21544. DOI:10.1002/arch.21544
[2] Nicholson WL,Sonenshine DE,Lane RS,et al. Ticks (ixodida)[M]//Mullen GR,Durden LA. Medical and Veterinary Entomology. Burlington:Academic Press,2009:493-542.
[3] 刘吉起,赵奇,许汴利. 蜱类研究进展[J]. 中国媒介生物学及控制杂志,2013,24(2):186-188. Liu JQ,Zhao Q,Xu BL. Progress in research on ticks[J]. Chin J Vector Biol Control,2013,24(2):186-188.
[4] Baneth G. Tick-borne infections of animals and humans:a common ground[J]. Int J Parasitol,2014,44(9):591-596. DOI:10.1016/j.ijpara.2014.03.011.
[5] 段炜. 中国蜱类系统分类学研究(蜱螨亚纲:蜱目)[D]. 北京:中国科学院大学,2013. Duan W. Taxonomic study of ticks in China (Acari:Ixodida)[D]. Beijing:University of Chinese Academy of Sciences,2013.
[6] 江莉,龚劲聪,胡龙飞,等. DNA条形码技术用于进口货物中肩突硬蜱的鉴定[J]. 中国国境卫生检疫杂志,2014,37(1):33-36. DOI:10.16408/j.1004-9770.2014.01.007. Jiang L,Gong JC,Hu LF,et al. Application of DNA barcode technology for detection of Ixodes scapularis from imported goods[J]. Chin J Front Health Quarant,2014,37(1):33-36. DOI:10.16408/j.1004-9770.2014.01.007.
[7] 侯海燕. COXⅠ基因DNA条形码技术在蜱类鉴别中的应用[J]. 国际检验医学杂志,2015,36(24):3570-3571. DOI:10.3969/J.ISSN.1673-4130.2015.24.026. Hou HY. Application of DNA barcoding technique of COXⅠgene in identification of ticks[J]. Int J Lab Med,2015,36(24):3570-3571. DOI:10.3969/J.ISSN.1673-4130.2015.24.026.
[8] 杨亚,刘进辉,伏健,等. 硬蜱常用分子分类技术研究进展[J]. 动物医学进展,2014,35(11):98-100. DOI:10.3969/j.issn. 1007-5038.2014.11.022. Yang Y,Liu JH,Fu J,et al. Advance in common molecular classification techniques of hard ticks[J]. Prog Vet Med,2014,35(11):98-100. DOI:10.3969/j.issn.1007-5038.2014.11.022.
[9] 赵丹云,孙毅,陈卫军,等. 天津口岸首次截获随人入境外来蜱的形态学及分子鉴定[J]. 中国国境卫生检疫杂志,2016,39(4):256-259. DOI:10.16408/j.1004-9770.2016.04.009. Zhao DY,Sun Y,Chen WJ,et al. Morphology and molecular identification of an imported tick with entry passenger intercepted at Tianjin port[J]. Chin J Front Health Quarant,2016,39(4):256-259. DOI:10.16408/j1004-9770.2016.04.009.
[10] 尹小平,叶志辉,骄娃,等. 阿拉山口口岸地区蜱类区系调查及宿主动物染带关系监测分析[J]. 中国媒介生物学及控制杂志,2010,21(4):375-377. Yin XP,Ye ZH,Jiao W,et al. Tick fauna and monitoring of tick host animals at Alataw pass[J]. Chin J Vector Biol Control,2010,21(4):375-377.
[11] 徐军,王安东,戴莉,等. 中哈边境阿拉山口口岸输入性亚洲璃眼蜱的种类鉴定与分析[J]. 中国兽医杂志,2016,52(11):17-19. Xu J,Wang AD,Dai L,et al. Species identification of an imported tick sample of Hyalomma asiaticum asiaticum by genetic analysis at Alashankou port,China-Kazakhstan border[J]. Chin J Vet Med,2016,52(11):17-19.
[12] 赵爽,胡文海,闫清丽,等. 南沙口岸首次从入境牛皮中截获盾陷璃眼蜱[J]. 中国媒介生物学及控制杂志,2014,25(5):452-454. DOI:10.11853/j.issn.1003.4692.2014.05.017. Zhao S,Hu WH,Yan QL,et al. Investigation of Hyalomma excavatum Koch first captured at Nansha port,Guangzhou,China[J]. Chin J Vector Biol Control,2014,25(5):452-454. DOI:10.11853/j.issn.1003.4692.2014.05.017.
[13] 吕洁毅,何振毅,刘谢,等. 佛山口岸首次截获绚丽花蜱[J]. 寄生虫与医学昆虫学报,2016,23(4):237-241. DOI:10.3969/j.issn.1005-0507.2016.04.008. Lyu JY,He ZY,Liu X,et al. An exotic species of Amblyomma intercepted at Foshan frontier port[J]. Acta Parasitol Med Entomol Sin,2016,23(4):237-241. DOI:10.3969/j.issn.1005-0507.2016.04.008.
[14] 余招锋,徐慧,陈鹏程,等. 一批泰国进境板材中截获的囊形扇头蜱的鉴定和检疫[J]. 中国动物检疫,2017,34(2):58-60. DOI:10.3969/j.issn.1005-944X.2017.02.016. Yu ZF,Xu H,Chen PC,et al. Identification and quarantine towards Rhipicephalus bursa in intercepted timbers imported from Thailand[J]. China Anim Health Inspect,2017,34(2):58-60. DOI:10.3969/j.issn.1005-944X.2017.02.016.
[15] 李树清,王强,林颍峥,等. 南非进口羊毛携带蜱的监测与伊文斯扇头蜱鉴定[J]. 中国媒介生物学及控制杂志,2019,30(4):414-417. DOI:10.11853/j.issn.1003.8280.2019.04.013. Li SQ,Wang Q,Lin YZ,et al. Surveillance of intercepted ticks carried by wool imported from South Africa and identification of Rhipicephalus evertsi[J]. Chin J Vector Biol Control,2019,30(4):414-417. DOI:10.11853/j.issn.1003.8280.2019.04.013.
[16] 邓国藩,姜在阶. 中国经济昆虫志. 第39册. 蜱螨亚纲:硬蜱科[M]. 北京:科学出版社,1991:1-43. Deng GF,Jiang ZJ. Economic insect fauna of China. Fac. 39 Acari:Ixodidae[M]. Beijing:Science Press,1991:1-43.
[17] 吕继洲,赵丽,赵昱林,等. 拉合尔钝缘蜱的形态学及分子生物学鉴定[J]. 中国畜牧兽医,2017,44(4):1149-1158. DOI:10.16431/j.cnki.1671-7236.2017.04.030. Lyu JZ,Zhao L,Zhao YL,et al. Morphological and molecular biology identification of Ornithodoros lahorensis[J]. China Anim Husband Vet Med,2017,44(4):1149-1158. DOI:10.16431/j.cnki.1671-7236.2017.04.030.
[18] 吕继洲,王振宝,袁向芬,等. 草原革蜱和边缘革蜱的分子生物学鉴定[J]. 中国畜牧兽医,2013,40(9):8-15. DOI:10.3969/j.issn.1671-7236.2013.09.002. Lyu JZ,Wang ZB,Yuan XF,et al. Molecular biological identification of Dermacentor nuttalli and D. marginatus[J]. China Anim Husband Vet Med,2013,40(9):8-15. DOI:10. 3969/j.issn.1671-7236.2013.09.002.
[19] Folmer O,Black M,Hoeh W,et al. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol,1994,3(5):294-299.
[20] Lyu JZ,Wu SQ,Zhang YN,et al. Development of a DNA barcoding system for the Ixodida (Acari:Ixodida)[J]. Mitochondrial DNA,2014,25(2):142-149. DOI:10.3109/19401736.2013.792052.
[21] 艾力西热·买买提,巴音查汗,吴辉,等. 拉合尔钝缘蜱的鉴定及其感染绵羊的调查[J]. 中国兽医杂志,2017,53(5):6-8. Mehmet A,BAYin-chahan,Wu H,et al. Identification of Ornithodoros lahorensis and its infectious investigation on sheep[J]. Chin J Vet Med,2017,53(5):6-8.
[22] 张际文. 中国国境口岸医学媒介生物鉴定图谱[M]. 天津:天津科学技术出版社,2015:443. Zhang JW. The identification atlas of medical vectors in Chinese points of entry[M]. Tianjin:Tianjin Science and Technology Press,2015:443.
[23] 陆继爽,格日勒图. 非洲猪瘟流行病学研究进展[J]. 中国畜牧兽医,2015,42(12):3377-3382. DOI:10.16431/j.cnki.1671-7236.2015.12.039. Lu JS,Geriletu. Research progress on epidemiology of African swine fever[J]. China Anim Husband Vet Med,2015,42(12):3377-3382. DOI:10.16431/j.cnki.1671-7236.2015.12.039.
[24] Walker AR,Bouattour A,Camicas JL,et al. Ticks of domestic animals in Africa:a guide to identification of species[M]. Edinburgh Scotland,UK:Bioscience Reports,2014:83-85.