Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China

doi:10.11853/j.issn.1003.8280.2019.05.010

Abstract

Abstract: Objective To analyze genetic mutations, predict the level of resistance to pyrethroid insecticides in the bedbug Cimex lectularius and provide a guide for bed bug control in this region. Methods Partial sequences of vgsc gene that encodes the target of pyrethroid insecticides (voltage gated sodium channel) were obtained by DNA sequencing, and the knockdown resistance-related mutations were examined. Results The six bed bugs collected on site were found to have genetic mutations at codons 419 and 925, leading to double amino acid substitutions (V419L and L925I) with a frequency of 100%. Conclusion All the investigated bed bugs carry kdr mutations at positions 419 and 925 of the voltage gated sodium channel, predicting that this bed bug population has a higher level of resistance to pyrethroid insecticides.

Key words: Cimex lectularius, Voltage-gated sodium channel, Genetic mutation, Knockdown resistance, Pyrethroid insecticides

摘要： 目的对温带臭虫电压门控钠离子通道（vgsc）基因进行测序分析，检测抗药性相关的基因突变，为该地区的臭虫治理提供依据。方法利用DNA测序技术，获得vgsc基因片段序列，分析击倒抗性相关位点的基因型。结果与参照品系相应的序列比对，现场采集的6只温带臭虫的vgsc基因序列均存在有义突变，导致编码的电压门控钠离子通道（VGSC）的氨基酸替换，即V419L和L925I突变，突变频率为100%。结论调查的样品均携带可导致击倒抗性的VGSC在419和925两个位点的突变，表明该温带臭虫种群对拟除虫菊酯类杀虫剂具有较高水平抗性。

关键词: 温带臭虫, 电压门控钠离子通道, 基因突变, 击倒抗性, 拟除虫菊酯类杀虫剂

CLC Number:

LIU Nian, ZHOU Xiao-jie, QI Guang-hao, LI Mei, QIU Xing-hui. Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(5): 524-527.

刘念, 周小洁, 戚广浩, 李梅, 邱星辉. 北京市温带臭虫击倒抗性基因突变检测[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 524-527.

References

[1] Reinhardt K,Siva-Jothy MT. Biology of the bed bugs (Cimicidae)[J]. Ann Rev Entomol,2007,52:351-374. DOI:10.1146/annurev. ento.52.040306.133913.
[2] Wang CL,Singh N,Cooper RA,et al. Evaluation of an insecticide dust band treatment method for controlling bed bugs[J]. J Econ Entomol,2013,106(1):347-352. DOI:10.1603/EC12259.
[3] Susser SR,Perron S,Fournier M,et al. Mental health effects from urban bed bug infestation (Cimex lectularius L.):a cross-sectional study[J]. BMJ Open,2012,2(5):e000838. DOI:10.1136/bmjopen-2012-000838.
[4] Dong K,Du YZ,Rinkevich F,et al. Molecular biology of insect sodium channels and pyrethroid resistance[J]. Insect Biochem Mol Biol,2014,50:1-17. DOI:10.1016/j.ibmb.2014.03.012.
[5] Sayono S,Hidayati APN,Fahri S,et al. Distribution of voltage-gated sodium channel (Nav) alleles among the Aedes aegypti populations in central Java province and its association with resistance to pyrethroid insecticides[J]. PLoS One,2016,11(3):e0150577. DOI:10.1371/journal.pone.0150577.
[6] Dang K,Doggett SL,Singham GV,et al. Insecticide resistance and resistance mechanisms in bed bugs,Cimex spp. (Hemiptera:Cimicidae)[J]. Parasit Vectors,2017,10(1):318. DOI:10.1186/s13071-017-2232-3.
[7] Palenchar DJ,Gellatly KJ,Yoon KS,et al. Quantitative sequencing for the determination of kdr-type resistance allele (V419L,L925I,I936F) frequencies in common bed bug (Hemiptera:Cimicidae) populations collected from Israel[J]. J Med Entomol,2015,52(5):1018-1027. DOI:10.1093/jme/tjv103.
[8] Zhu F,Wigginton J,Romero A,et al. Widespread distribution of knockdown resistance mutations in the bed bug,Cimex lectularius (Hemiptera:Cimicidae),populations in the United States[J]. Arch Insect Biochem Physiol,2010,73(4):245-257. DOI:10.1002/arch.20355.
[9] Campbell BE,Koehler PG,Buss LJ,et al. Recent documentation of the tropical bed bug (Hemiptera:Cimicidae) in Florida since the common bed bug resurgence[J]. Fla Entomol,2016,99(3):549-551. DOI:10.1653/024.099.0333.
[10] 王陇德. 病媒生物防制实用指南[M]. 北京:人民卫生出版社,2010:94-96.
[11] Rinkevich FD,Zhang L,Hamm RL,et al. Frequencies of the pyrethroid resistance alleles of Vssc1 and CYP6D1 in house flies from the eastern United States[J]. Insect Mol Biol,2006,15(2):157-167. DOI:10.1111/j.1365-2583.2006.00620.x.
[12] Potter MF. The history of bed bug management-with lessons from the past[J]. Am Entomol,2011,57(1):14-25. DOI:10.1093/ae/57.1.14.
[13] Doggett SL,Russell RC. The resurgence of bed bugs,Cimex spp. (Hemiptera:Cimicidae) in Australia:experiences from down Under[C]//Proceedings of the 6th International Conference on Urban Pests. Pápai,Hungary:OOK Press,2008:407-425.
[14] Romero A,Potter MF,Potter DA,et al. Insecticide resistance in the bed bug:a factor in the pest's sudden resurgence?[J]. J Med Entomol,2007,44(2):175-178. DOI:10.1093/jmedent/44.2.175.
[15] Yoon KS,Kwon DH,Strycharz JP,et al. Biochemical and molecular analysis of deltamethrin resistance in the common bed bug (Hemiptera:Cimicidae)[J]. J Med Entomol,2008,45(6):1092-1101. DOI:10.1093/jmedent/45.6.1092.
[16] Dang K,Toi CS,Lilly DG,et al. Detection of knockdown resistance mutations in the common bed bug,Cimex lectularius (Hemiptera:Cimicidae),in Australia[J]. Pest Manag Sci,2015,71(1):914-922. DOI:10.1002/ps.3861.